Isolation of Eyach virus (Reoviridae, Colorado tick fever group) fromIxodes ricinus andI. ventalloi ticks in France

Chastel, C; Main, Andrew J.; Couatarmanach, Antoine; Lay, G. Le; Knudson, D. L.; Quillien, M. C.; Beaucournu, Jean-Claude

doi:10.1007/bf01311160

Cited by 74 publications

(41 citation statements)

References 20 publications

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“…Strain $6-14-03, from a hare, Lepus calijbrnicus, collected in northwestern California, is distinct from CTF (Florio) virus and more closely related to Eyach virus isolated in West Germany (Rehse-Kupper et al, 1976) and France (Chastel et al, 1984). Another virus isolate from California (T5-2092; western grey squirrel, Sciurus griseus) is identical by PRNT to strain $6-14-03 (data not shown).…”

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confidence: 75%

Antigenic Variants of Colorado Tick Fever Virus

Karabatsos

Poland

Emmons

et al. 1987

Journal of General Virology

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SUMMARYTwenty strains of Colorado tick fever (CTF) virus, isolated from ticks, mammals and humans, and two antigenic relatives of CTF virus were compared in crossneutralization tests. Viruses were tested using single-inoculation sera prepared in hamsters. Antigenic variation, as measured by differences detected in the neutralization test, was noted among the virus isolates identified as strains of CTF virus. The virus strains isolated from humans appeared to vary the most in serological reactions. The two antigenic relatives of CTF virus are clearly distinct from strains of CTF and are different from each other. Antigenic relationships between these two viruses were established using two sets of single-inoculation antisera and both complement fixation and neutralization tests. Six distinct antigenic variants of CTF virus isolated from humans and the virus strain from ticks (75V1906) that showed the least antigenic variation, were tested against 49 coded serum pairs from clinically diagnosed cases of CTF. Significant differences were noted in the number of convalescent-phase sera that reacted with each virus strain and in the number of seroconversions observed with each test virus strain. Convalescent phase sera that reacted with multiple virus strains often varied significantly in antibody titre from one virus strain to another. This indicates that, in some instances, antibody was probably produced in response to infection by different antigenic variants of CTF virus.

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confidence: 75%

Antigenic Variants of Colorado Tick Fever Virus

Karabatsos

Poland

Emmons

et al. 1987

Journal of General Virology

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“…Among viruses exhibiting a dsRNA 12 segment genome, those that infect plants are grouped within the genus Phytoreovirus and those isolated from vertebrates, ticks and mosquitoes are classified in the genus Coltivirus. The antigenic relationship between CTF (American) and Eyach (European) virus isolates has been analysed (Chastel et al, 1984 ;Karabatsos et al, 1987 ;RehseKu$ pper et al, 1976). Neutralization of both American and European isolates by antibodies to CTF virus (Karabatsos et al, 1987) suggests that these viruses are antigenically related.…”

Section: Discussionmentioning

confidence: 99%

“…Other members of the genus are Eyach virus, isolated in Germany (Rehse- Ku$ pper et al, 1976), and two Eyach virus antigenic variants (AR577 and AR578), isolated in France (Chastel et al, 1984). In previous studies, an antigenic relationship between these American and European isolates was established (Chastel et al, 1984 ;Karabatsos et al, 1987) and full-length nucleotide sequences of genomic segments 10, 11 and 12 of CTF virus were determined (Attoui et al, 1997). Other viruses are also considered as tentative species of the genus, e.g.…”

Section: Introductionmentioning

confidence: 99%

Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus.

Attoui¹,

Charrel²,

Billoir³

et al. 1998

Journal of General Virology

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In this study, the basis for the classification of virus isolates grouped within the genus Coltivirus, family Reoviridae, is discussed. Sequences of dsRNA segments from American (segments 9-12), European (segment 12) and Asian (segments 7-12) isolates were characterized and polythetic criteria were defined for their taxonomic classification. These criteria (including sequence analysis) permitted the different species to be distinguished and classified into two groups. In both groups, subgroups were defined according to the degree of homology between the genomic sequences. American and European isolates are classified within

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“…Such a difference in the specificity of the used primers could allow a differential diagnosis of the European and the American strains of Coltiviruses. The Ar/T578 and Eyach viruses were serologically investigated by Chastel and others 11 and Karabatsos and others 16 , respectively, and the investigators concluded that these viruses were antigenically distinct from CTF virus but related to each other. The results of immunofluorescence studies we conducted on C6/ 36 cells infected with the Ar/T578 virus using anti-CTF virus immune ascitic fluid showed an appreciable crossreactivity with the CTF virus.…”

Section: Discussionmentioning

confidence: 99%

“…In 1990, the genus Coltivirus was created within the family Reoviridae, encompassing those viruses isolated from vertebrates and invertebrates exhibiting a 12-segment double-stranded (ds) RNA genome. At present, this genus includes the CTF virus, the Eyach virus isolated in Germany in 1976, 10 the two Eyach antigenic variants Ar/T577 and Ar/T578 that were isolated in France in 1981 11 , and other members isolated in Indonesia 12 and China. 13 The Eyach virus has been suspected to be involved in some neurologic manifestations since antibodies to this virus were detected in sera of patients with polyradiculonevritis.…”

mentioning

confidence: 99%

Serologic and molecular diagnosis of Colorado tick fever viral infections.

Attoui¹,

Billoir²,

Bruey³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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Abstract. Molecular and serologic methods usable for the biological diagnosis of Coltivirus infection are reported. We designed a multiplex reverse transcription-polymerase chain reaction system that allowed the simultaneous and specific amplification of three genomic segments from as little as 0.01 plaque-forming units. Another system in the S2 viral segment permitted the differential diagnosis of American and European viral isolates. We also discuss some improvements of previous ELISAs, and the results obtained with paired sera from Colorado tick fever (CTF) virusinfected individuals. Western blot analysis was developed that allowed the detection of antibodies to a 38-kD viral protein in all tested sera. It also enabled the detection of anti-CTF virus antibodies in ELISA-negative sera. Specific IgM antibodies against a synthetic viral peptide could be detected in sera at the acute stage of the infection. Together, these results should permit the diagnosis of Coltivirus infection at any stage of the pathology.Colorado tick fever (CTF) is a severe tick-borne disease that is usually self-limited. This disease occurs in 11 western states in the United States and has also been reported in Canada. 1 Yearly, 200-400 cases are reported throughout the endemic states in the United States. 1,2 The clinical manifestations appear following the bite of the infected adult Rocky Mountain wood tick Dermacentor andersoni 3 and were first reported in the Rocky Mountain region. 1 It has an abrupt onset with flu-like manifestations and some complications of the disease have been reported, particularly in children. [4][5][6][7] Since the first reports in 1875 to reports of Keiffer and Becker in 1906 and 1926, respectively, 8 on a tick bite-generated biphasic fever having clinical symptoms that does not conform to those of Rocky Mountain spotted fever (RMSF), CTF was recognized as being a separate clinical entity from RMSF. Furthermore, the isolation and characterization of the causative agent, CTF virus, by Florio and others 9 in the 1940s established the viral etiology of the disease. In 1990, the genus Coltivirus was created within the family Reoviridae, encompassing those viruses isolated from vertebrates and invertebrates exhibiting a 12-segment double-stranded (ds) RNA genome. At present, this genus includes the CTF virus, the Eyach virus isolated in Germany in 1976, 10 the two Eyach antigenic variants Ar/T577 and Ar/T578 that were isolated in France in 1981 11 , and other members isolated in Indonesia 12 and China. 13The Eyach virus has been suspected to be involved in some neurologic manifestations since antibodies to this virus were detected in sera of patients with polyradiculonevritis. 14 The diagnosis of CTF virus infection is made using various procedures. Isolation and characterization by animal inoculation and neutralization were reported to be the most reliable techniques. 8,15 However, serologic detection by ELI-SA has also been developed. 15 Moreover, many strains were reported 16, 17 that could be detected by RN...

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Isolation of Eyach virus (Reoviridae, Colorado tick fever group) fromIxodes ricinus andI. ventalloi ticks in France

Cited by 74 publications

References 20 publications

Antigenic Variants of Colorado Tick Fever Virus

Antigenic Variants of Colorado Tick Fever Virus

Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus.

Serologic and molecular diagnosis of Colorado tick fever viral infections.

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