Isolation of Fisetin from Elaeagnus indica Serv. Bull. (Elaeagnaceae) with antioxidant and antiproliferative activity

Ramalingam, Srinivasan; Shivakumar, M.; Nagamurugan, N.

doi:10.5530/fra.2016.2.3

Cited by 14 publications

(5 citation statements)

References 34 publications

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“…The chemical structures of the identified compounds were verified by 1 H NMR, 13 C NMR, and 2D NMR with heteronuclear single quantum correlation (HSQC) and heteronuclear multiple bond correlation (HMBC) using an NMR 700 MHz instrument (Bruker Corporation, Billerica, MA, USA). 26 The measured samples were dissolved in DMSO‐d 6 . Active components were quantified using EXTREMA (Japan Spectroscopy Co., Ltd., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Fisetin, a major component derived from mulberry (Morus australis Poir.) leaves, prevents vascular abnormal contraction

2021

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Mulberry (Morus australis Poir.) leaves have long been consumed in the form of tea or tincture especially in Asia, owing to their high antioxidant and blood pressure-regulating properties. Although it is thought that vascular abnormal contraction may be involved in the blood pressure-suppressing effect, the effect of mulberry on vascular abnormal contraction is still unknown. Therefore, we investigated mulberry leaves as a potential source of bioactive compounds that prevent vascular abnormal contraction. Mulberry leaves were divided into fresh leaves and tea leaves and further classified according to the age of the tree: more or less than 20 years old, into roasted and unroasted. Mulberry fruits were also evaluated.We assessed the preventive effect of mulberry extracts on vascular abnormal contraction. Extracts from mulberry leaves of trees more than 20 years old showed a strong preventive effect on vascular abnormal contraction of human coronary artery smooth muscle cells. Therefore, to identify the active components in mulberry leaves, we fractionated the active fractions by gel filtration chromatography and reversed-phase high-performance liquid chromatography. The active fraction was further analyzed by mass spectrometry and nuclear magnetic resonance; an active component of the mulberry leaf extract was fisetin. In addition, our results indicated that the hydroxyl group at the C-3 position of fisetin is crucial for its activity. These results prove that fisetin is effective in preventing vascular abnormal contraction. Overall, mulberry leaves and fisetin are expected to be used in a wide range of fields such as functional foods, nutraceuticals, and drug targets.

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Section: Methodsmentioning

confidence: 99%

Fisetin, a major component derived from mulberry (Morus australis Poir.) leaves, prevents vascular abnormal contraction

2021

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“…Fisetin ( Figure 4W ) is a 7-hydroxyflavonol that can be obtained from various pigmented fruits and vegetables, like Elaeagnus indica Servett. ( Srinivasan et al, 2016 ), Hymenaea courbaril L. (jatoba) ( da Costa et al, 2014 ), and Toxicodendron vernicifluum (Stokes) F.A.Barkley ( Lee JH. et al, 2015 ).…”

Section: Natural Products Against Sars-cov-2: Computational To Preclinical Studiesmentioning

confidence: 99%

Natural Products for the Prevention and Control of the COVID-19 Pandemic: Sustainable Bioresources

Singla

Chopra

et al. 2021

Front. Pharmacol.

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Background: The world has been unprecedentedly hit by a global pandemic which broke the record of deadly pandemics that faced humanity ever since its existence. Even kids are well-versed in the terminologies and basics of the SARS-CoV-2 virus and COVID-19 now. The vaccination program has been successfully launched in various countries, given that the huge global population of concern is still far behind to be vaccinated. Furthermore, the scarcity of any potential drug against the COVID-19-causing virus forces scientists and clinicians to search for alternative and complementary medicines on a war-footing basis.Aims and Objectives: The present review aims to cover and analyze the etiology and epidemiology of COVID-19, the role of intestinal microbiota and pro-inflammatory markers, and most importantly, the natural products to combat this deadly SARS-CoV-2 virus.Methods: A primary literature search was conducted through PubMed and Google Scholar using relevant keywords. Natural products were searched from January 2020 to November 2020. No timeline limit has been imposed on the search for the biological sources of those phytochemicals. Interactive mapping has been done to analyze the multi-modal and multi-target sources.Results and Discussion: The intestinal microbiota and the pro-inflammatory markers that can serve the prognosis, diagnosis, and treatment of COVID-19 were discussed. The literature search resulted in yielding 70 phytochemicals and ten polyherbal formulations which were scientifically analyzed against the SARS-CoV-2 virus and its targets and found significant. Retrospective analyses led to provide information about 165 biological sources that can also be screened if not done earlier.Conclusion: The interactive analysis mapping of biological sources with phytochemicals and targets as well as that of phytochemical class with phytochemicals and COVID-19 targets yielded insights into the multitarget and multimodal evidence-based complementary medicines.

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“…A total of two fractions (GPSC-55SF and GPSC-73SF) exhibited considerable antibacterial activity; these fractions again were subjected to preparative TLC purification (20). The preparative TLC plate was performed based on the noted R f values.…”

Section: Separation Of Active Compounds By Chromatographymentioning

confidence: 99%

Glycosmis pentaphylla (Rutaceae): A Natural Candidate for the Isolation of Potential Bioactive Arborine and Skimmianine Compounds for Controlling Multidrug-Resistant Staphylococcus aureus

Murugan

Ramalingam

Athiappan

et al. 2020

Front. Public Health

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Several multidrug-resistant organisms have emerged, which increases the threat to public health around the world and a limited number of therapeutics were available to counteract these issues. Thus, researchers are trying to find out novel antimicrobials to overcome multidrug-resistant issues. The present study aimed to isolate antibacterial principles against the clinical isolates of multidrug-resistant (MDR) Staphylococcus aureus from the ethyl acetate extract of Gycosmis pentaphylla. The isolation and structural characterization of bioactive compounds were carried out using various chromatographic techniques (TLC, column, HPLC, and LC-MS) and spectral studies such as FT-IR, CHNS analysis, 1 H-NMR, and 13 C-NMR. The antimicrobial potential of isolated compounds was assessed according to the standard methods. The isolated compounds were identified as arborine and skimmianine, which exhibited a significant antibacterial effect with the lowest MIC and MBC values against MDR S. aureus and in vitro kinetic and protein leakage assays supported the antimicrobial activity. Significant morphological changes such as uneven cell surfaces and morphology, cell shrinkage, and cell membrane damages were observed in the MDR S. aureus upon the treatment of arborine and skimmianine. The present investigation concludes that the isolated arborine and skimmianine compounds from G. pentaphylla harbor a strong antibacterial activity against MDR S. aureus and may be used as alternative natural drugs in the treatment of MDR S. aureus.

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Isolation of Fisetin from Elaeagnus indica Serv. Bull. (Elaeagnaceae) with antioxidant and antiproliferative activity

Cited by 14 publications

References 34 publications

Fisetin, a major component derived from mulberry (Morus australis Poir.) leaves, prevents vascular abnormal contraction

Fisetin, a major component derived from mulberry (Morus australis Poir.) leaves, prevents vascular abnormal contraction

Natural Products for the Prevention and Control of the COVID-19 Pandemic: Sustainable Bioresources

Glycosmis pentaphylla (Rutaceae): A Natural Candidate for the Isolation of Potential Bioactive Arborine and Skimmianine Compounds for Controlling Multidrug-Resistant Staphylococcus aureus

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