Konsentrasi Hambat Minimum (KHM) Kadar Sampel Alang-Alang (Imperata cylindrica) dalam Etanol Melalui Metode Difusi Cakram

Mulyadi, Moh.; Wuryanti, Wuryanti; Sarjono, Purbowatiningrum Ria

doi:10.14710/jksa.20.3.130-135

Cited by 27 publications

(26 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Where as in Gram negative bacteria, the compounds in the extract cannot directly bind with peptidoglycan but must damage the outer membrane first. Its cause of Gram-negative bacteria is more difficult to inhibit than gram-positive bacteria (Mulyadi, et al 2017). Table 4.…”

Section: Antibacterial Test Results For Paper Disc Methodsmentioning

confidence: 99%

Bioactivity of Khail-khail (Elaeagnus latifolia) Leaf Extract to Bacteria Staphylococcus saprophyticus and Salmonella enterica

Roza¹,

Sutiani²,

Meilano³

2019

Proceedings of the Proceedings of the 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018

View full text Add to dashboard Cite

Khail-khail (Elaeagnus latifolia) is one of the plants endemic to Samosir Island in North Sumatra. Nowadays, further research was needed to explore bioactive compounds produced by these plants. This study aimed to assess the antibacterial activity of plant extract against Staphylococcus saprophyticus and Salmonella enterica. The methods of antibacterial activity test were Paper Discs and Microdilution. The Antibacterial activity results used paper disc method against Staphylococcus saprophyticus showed a weak inhibition and Resistant (R). Moreover, the MIC and KBM values with microdilution method were 1250 μg/mL.

show abstract

Section: Antibacterial Test Results For Paper Disc Methodsmentioning

confidence: 99%

Bioactivity of Khail-khail (Elaeagnus latifolia) Leaf Extract to Bacteria Staphylococcus saprophyticus and Salmonella enterica

Roza¹,

Sutiani²,

Meilano³

2019

Proceedings of the Proceedings of the 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018

View full text Add to dashboard Cite

show abstract

“…The inhibitory activity results from lignin photodegradation of 700 ppm is a relatively strong inhibitory, based on Mulyadi et al that the mushroom or microbial growth resistance response is less effective when the clear zone diameter is <10 mm, weak if it ranges from 10-15 mm, medium between 16-20 mm, and strong above equal to 20 mm 23 .…”

Section: Antimicrobial Activity Of Lignin Degradation Compoundsmentioning

confidence: 99%

Natural Biopesticide Preparation as Antimicrobial Material Based on Lignin Photodegradation Using Mineral Ilmenite (FEO.TIO2)

Natsir¹,

Maulidiyah²,

Ansharullah³

et al. 2018

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Natural biopesticide has been prepared by lignin photodegradation for antimicrobial activity on Fusarium oxysporum and Xanthomonas sp. The photodegradation process in UV reactor was performed by FeO.TiO2 mass and lignin concentration variations. The results showed that lignin concentration on 500 ppm and 700 ppm with 0.2 g FeO.TiO2 as an optimum mass to degrade lignin were 20.63% and 27.17%. Based on antimicrobial activity test against Fusarium oxysporum showed that the lignin degradation compound in 300 ppm, 500 ppm, and 700 ppm were 11.67 mm, 13 mm, and 21 mm diameter zone. Meanwhile, the results of lignin degradation compound have a good activity test of Xanthomonas sp. for 300 ppm, 500 ppm, and 700 ppm with inhibitory zone were 17.6 mm, 18.33 mm, and 19.66 mm, respectively. These results indicated that the lignin photodegradation for 700 ppm using FeO.TiO2 has produced activity with a strong inhibitory zone against Fusarium oxysporum and Xanthomonas sp.

show abstract

“…Hasil Analysis of Variance (ANOVA), menunjukkan nilai F = 7387.32 dan p-value (nilai probabilitas) = 0.0001 signifikan pada tingkat signifikasi α = 0.05. (Mulyadi et al, 2013). Hasil penelitian menunjukkan bahwa ekstrak daun pepaya dalam berbagai level memberikan pengaruh sangat nyata (nilai probabilitas < 0.001) terhadap diameter hambat bakteri Staphylococcus aureus yang dapat dilihat pada Gambar 2 yang menunjukkan respon Staphylococcus aureus terhadap dosis ekstrak daun pepaya.…”

Section: Analysis Of Variance (Anova) Staphylococcus Aureusunclassified

“…Metode cakram kertas yaitu meletakkan cakram kertas yang telah direndam larutan uji di atas media padat yang telah diinokulasi dengan bakteri. Setelah diinkubasi, pertumbuhan bakteri diamati untuk melihat ada tidaknya daerah hambatan disekeliling cakram (Mulyadi et al, 2013). Hasil penelitian menunjukkan bahwa ekstrak daun pepaya dalam berbagai level memberikan pengaruh sangat nyata (nilai p < 0.001) terhadap diameter hambat bakteri Escherichia coli yang dapat dilihat pada Gambar 3.…”

Section: Respon Bakteri Escherichia Coli Terhadap Dosis Ekstrak Daun unclassified

Optimasi Durasi Dan Rasio Bahan Per Pelarut Ekstrak Daun Pepaya Untuk Uji Aktivitas Antibakteri

Sabathani

Widjanarko

Yuwono

2018

JTP

View full text Add to dashboard Cite

ABSTRAKTujuan penelitian ini adalah mengetahui kombinasi waktu sonikasi dan bahan per pelarut (b/v) ekstrak daun pepaya segar, yang menghasilkan respon total fenol, aktivitas antioksidan (IC 50 ), dan rendemen yang optimum; juga untuk mengetahui efek dari dosis ekstrak daun pepaya pada aktivitas antibakteri Escherichia coli dan Staphylococcus aureus. Ekstrak daun pepaya dihasilkan menggunakan metode ultrasonik dengan pelarut aquades. Rancangan penelitian menggunakan Response Surface Method, sedangkan desain eksperimen menggunakan General Factor Design, dengan satu faktor yang terdiri dari 6 tingkat dosis ekstrak daun pepaya, kontrol positif dan negatif, diulang 3 kali. Kontrol positif adalah kertas cakram mengandung 10 μg ampisilin untuk bakteri Staphylococcus aureus, kertas-cakram mengandung 30 μg kloramfenikol untuk Escherichia coli, sedangkan kontrol negatif adalah aquades steril. Hasil penelitian menunjukkan kombinasi b/v 16.92 g/100 ml dengan lama waktu ekstraksi daun pepaya 14.36 menit dan menghasilkan tiga respon optimum rendemen 2.89%, total fenol 50.03 mgGAE/g, dan aktivitas antioksidan (IC 50 ) 211.924 ppm. Ekstrak daun pepaya pada konsentrasi 3.125 mg/ml, 6.25 mg/ml, dan 12.5 mg/ml tidak berpengaruh terhadap perkembangan bakteri Staphylococcus aureus dan Escherichia coli, dan berpengaruh mulai 25 mg/ml, 50 mg/ml, 100 mg/ml. Hal ini menunjukkan bahwa ekstrak daun pepaya berpotensi menahan perkembangan bakteri Staphylococcus aureus dan Escherichia coli Kata kunci: Optimasi; Daun Pepaya; Ekstrak; Uji Aktivitas Antibakteri ABSTRACTThe aim of this research is to determine the combination of time duration of sonication and the ratio of the weight of material per solvent of fresh papaya leaf, which can produce optimum response of total phenol, antioxidant activity (IC 50 ), and rendemen; and to figure out the effect of dose of papaya leaf extract on antibacterial activity, both Escherichia coli and Staphylococcus aureus. The papaya leaf extract is resulted by using ultrasonic method with water solvent. The experiment is designed by using response surface method and general factor design, with one factor consist of 6 levels of papaya leaf extract dose, negative, and positive control and is repeated 3 times. The positive control is disc-paper containing 10 μg ampicillin for Staphylococcus aureus, disc-paper containing 30 μg chloramphenicol for Escherichia coli. The negative control is sterile aquadest. The results of the study shows a combination of the ratio the weight material per solvent 16.92 g/ 100 ml with the duration of papaya leaf extraction 14.36 minutes and yields three optimum responses: rendemen of 2.89%, total phenol of 50.03 mgGAE/g, and antioxidant activity (IC 50 ) 211.924 ppm, furthermore papaya leaf extract at concentrations of 3.125 mg/ml, 6.25 mg/ml, and 12.5 mg/ml does not have any effect on the development of Staphylococcus aureus and Escherichia coli bacteria, and only has an effect of 25 mg/ml, 50 mg/ml, 100 mg/ml. This suggests that papaya leaf extract has the potential to withstand the...

show abstract

Konsentrasi Hambat Minimum (KHM) Kadar Sampel Alang-Alang (Imperata cylindrica) dalam Etanol Melalui Metode Difusi Cakram

Abstract: A r t i c l e I n f oA b s t r a c t

Cited by 27 publications

References 1 publication

Bioactivity of Khail-khail (Elaeagnus latifolia) Leaf Extract to Bacteria Staphylococcus saprophyticus and Salmonella enterica

Bioactivity of Khail-khail (Elaeagnus latifolia) Leaf Extract to Bacteria Staphylococcus saprophyticus and Salmonella enterica

Natural Biopesticide Preparation as Antimicrobial Material Based on Lignin Photodegradation Using Mineral Ilmenite (FEO.TIO2)

Optimasi Durasi Dan Rasio Bahan Per Pelarut Ekstrak Daun Pepaya Untuk Uji Aktivitas Antibakteri

Contact Info

Product

Resources

About