<p>Polymer-Assisted In Situ Synthesis of Silver Nanoparticles with Epigallocatechin Gallate (EGCG) Impregnated Wound Patch Potentiate Controlled Inflammatory Responses for Brisk Wound Healing</p>

Abstract

IntroductionAn ideal wound dressing material needs to be predisposed with desirable attributes like anti-infective effect, skin hydration balance, adequate porosity and elasticity, high mechanical strength, low wound surface adherence, and enhanced tissue regeneration capability. In this work, we have synthesized hydrogel-based wound patches having antibacterial silver nanoparticles and antioxidant epigallocatechin gallate (EGCG) and showed fast wound closure through their synergistic interaction without any inherent toxicity.Methods and resultsWound patches were synthesized from modified guar gum polymer and assessed to determine accelerated wound healing. The modified polymer beget chemical-free in-situ synthesis of monodispersed silver NPs (~12 nm), an antimicrobial agent, besides lending ionic surface charges. EGCG impregnated during ionotropic gelation process amplified the efficacy of wound patches that possess apt tensile strength, porosity, and swellability for absorbing wound exudates. Further, in vitro studies endorsed them as non-cytotoxic and the post agent effect following exposure to the patch showed an unbiased response to E coli K12 and B. subtilis. In vivo study using sub-cutaneous wounds in Wistar rats validated its accelerated healing properties when compared to a commercially available wound dressing material (skin graft; Neuskin-F®) through better wound contraction, promoted collagen deposition and enhanced vascularization of wound region by modulating growth factors and inflammatory cytokines.ConclusionSynthesized wound patches showed all the desired attributes of a clinically effective dressing material and the results were validated in various in vitro and in vivo assays.

Application of Polymer Drugs with Cerium Dioxide Nanomolecules and Mesenchymal Stem Cells for the Treatment of Skin Wounds in Aged Rats

“…Thus, the effectiveness of the use of SPN and SPN + SC for the treatment of wounds has been determined. The results obtained are consistent with the opinion of earlier studies on the effective use of polymers [ 10 , 11 , 12 , 13 , 14 , 24 ] and molecules of cerium dioxide [ 20 , 21 , 22 , 23 , 24 , 25 ] for the treatment of wounds. In addition, our study confirms the results of other studies that the introduction of nanoparticles of certain substances radically changes the effectiveness of drugs, and this represents a quantum leap in providing safe and effective targeted drug delivery to the affected tissues [ 31 , 32 ].…”

“…The search began on organic or inorganic substances with an unknown effect on the cells of micro- or macro-organisms. In recent years, research data have begun to accumulate on the positive role of various polymers on wound healing [ 10 , 11 , 12 , 13 , 14 , 15 ]. In addition, cerium dioxide was found to be capable of exhibiting antioxidant properties in a cell culture or wound due to the ability to change its valence and stabilize the pH of the intercellular and wound environment [ 16 , 17 , 18 , 19 ].…”

Hydrogel Preparation Methods and Biomaterials for Wound Dressing

“…At an early stage of wound healing, IL-6 and IL-10 are responsible for the recruitment of fibroblast as well as the removal of extracellular matrix debris, production of pro-inflammatory molecules should subside after the inflammatory phase. The synergistic effect of each component of HG-Ag-EGCG hydrogels dressing not only accelerated the synthesis of various cytokines at the wound site, but also inhibited the survival of bacteria and excessive ROS and RNS molecules, so that the wound can transition smoothly to the proliferation and remodeling phase, thereby enhancing the repair process [ 80 ]. SA hydrogels are used as the main ingredient, embedded AgNPs, anti-inflammatory drugs, etc.…”

“…Based on HLC and CS, HCD hydrogels were synthesized by cross-linking dialdehyde starch. Various in vitro and in vivo evaluations show that HCD hydrogels have good biocompatibility and biodegradability, indicating that HCD may be a useful cosmetic dermal filler [ 80 ]. The antibacterial hydrogel dressing was prepared from marine fish scales made of aminated collagen with low immunogenicity and high biocompatibility, OSA, and antimicrobial peptides.…”

Biomedical Applications of Silver Nanoparticles (Review)

“…Анализ на антибактериальную активность выявил наибольшую эффективность гидрогелей, содержащих AgNP без добавления EGCG, в отношении грамположительных (B. subtilis, S. aureus) и грамотрицательных (E. coli, P. аeruginosa) бактерий, добавление EGCG незначительно снижало антибактериальный эффект. Однако добавление EGCG способствовало снижению количества провоспалительного фактора IL-6 и поддержанию базального уровня противовоспалительного цитокина IL-10, что важно для запуска процессов восстановления ткани [44]. Исследователями были получены гидрогели, обладающие антибактериальными свойствами в течение продолжительного периода времени, также способствующие быстрому заживлению раны за счет проявления противовоспалительных эффектов.…”