Magnetic immunoassay coupled with inductively coupled plasma mass spectrometry for simultaneous quantification of alpha-fetoprotein and carcinoembryonic antigen in human serum

Applications of Gold Nanoparticles in Non-Optical Biosensors

“…The technology offers extremely low detection limit and an extremely wide dynamic linear range with a working range more than 9 orders of magnitude, and owns the advantages of simple spectral lines, low interference, high analytical precision, rapid analysis and high specificity, which is widely used in environmental protection, biology, medicine, metallurgy, nuclear material analysis and other fields [137,138,139,140,141,142,143]. In the past few years, the strategies combining ICP-MS technology with metal nanoparticle labels were developed to achieve ultra-high sensitivity analysis in biomolecular analysis [144,145,146,147,148,149,150,151,152].…”

Immunoassays based on inductively coupled plasma mass spectrometry detection: So far so good, so what?

“…A large variety of NPs have been proposed (AgNPs [52][53][54][55], PtNPs [53,56], PbSNPs [57], metal/dye-doped silica NPs [58], Cs-doped magnetic NPs [58,59], UCNPs [30,31], EuPO 4 /LuPO 4 NPs [32], Quantum Dots (QDs) [60][61][62][63][64][65], etc.) but AuNPs [34,[53][54][55]63,[66][67][68][69][70][71][72][73][74][75][76] are usually preferred due to their lower cost and greater commercial availability. When compared to REE-based chelates, the use of inorganic NPs affords some attractive and unique features.…”

“…In general, NPs have been mainly employed for single analyte determinations [31,54,56,57,59,60,62,[64][65][66][67][69][70][71][74][75][76]. Unlike REEbased chelates, and despite the vast number of described NPs proposed in the literature for immunoassays, the use of NPs for multiplexed applications has been scarcely exploited [32,53,55,58,63]. For instance, Lores-Padín et al [53] have employed AuNCs, AgNCs and PtNCs for the simultaneous determination of metallothionein 1/2 (MT1/2), complement factor H and amyloid precursor protein in retina, ciliary body, retinal pigment epithelium, choroid and sclera from human cadaveric eye sections.…”

“…2A) can be directly performed using either isothiocyanate [29,34,35,46,47,79] or succinimide ester chemistry [17,30,31,52,53,[56][57][58][59][62][63][64][65]72,73]. Additionally, several authors have employed amide chemistry to directly tag antibodies with citrate-stabilized metallic NPs [55,63,68,70,78]. Nevertheless, it has been observed that, depending on experimental conditions (e.g.…”

A sensitive atomic absorption spectrometric metalloimmunoassay with copper nanoparticles labeling

“…Zhang et al [12] and Tanner et al [13] reported the pioneer work about various kinds of ICPMS-based metalloimmunoassays by Au nanoparticles [14][15][16][17][18][19][20][21][22] labeling. CdSe quantum dots [23], PbS quantum dots [24] and silver nanoparticles [25] were employed by Hu et al for disease biomarker quantification by ICPMS. HgS nanoparticles [26] were synthesized by Lv et al for high sensitivity in AFS.…”