Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-κB inhibitors and β2 adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris

Characterization of chemical constituents and rats metabolites of an alkaloidal extract of Alstonia scholaris leaves by liquid chromatography coupled with mass spectrometry

“…These picrinine-type alkaloids displayed maximum UV absorptions at 284 and 234 nm. Most of these alkaloids would produce abundant [M − C 2 H 5 N+H] + and [M − C 2 H 4 O 2 +H] + ions in the MS/MS spectra, which are different from other types of AAS alkaloids [14]. Take Pic (27) as example, the MS 2 spectra showed m/z 321 as the base peak, resulting from the loss of H 2 O unit (Fig.…”

“…The m/z 311 ion could further eliminate CH 3 N to produce m/z 282 as the base peak in the MS 3 spectra (Fig. 4A) [14,15].…”

Antibacterial monoterpenoid indole alkaloids from Alstonia scholaris cultivated in temperate zone

“…In our previous phytochemical studies, a series of monoterpenoid indole alkaloids were isolated from different parts of plant [7][8][9]. Besides, alkaloids from leaf of A. scholaris cultivated in Pu'er, indicated anti-tussive, anti-asthmatic, anti-inflammatory, analgesic and expectorant activities both in vitro and in vivo [10][11][12][13]. The remarkable difference of the alkaloidal patterns in A. scholaris in different ecological environment has been reported in 1990 [14].…”

“…Then, we investigated HPLC fingerprints of alkaloids of A. scholaris from Pu'er and Kunming, and the result exhibited visible difference, which encouraged us to search for structurally unique and biologically active indole alkaloids of A. scholaris from different climate zones. As a result, three new monoterpenoid indole alkaloids, normavacurine-21-one (1), 5-hydroxy-19, 20-E-alschomine (2), and 5-hydroxy-19, 20-Z-alschomine (3), together with thirteen known monoterpenoid indole alkaloids (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16), were…”

Identification of Alkaloid’s Profile in Ficus benjamina L. Extracts with Higher Antioxidant Power

“…Its use is common in agriculture field as insecticide, but its utility is limited because of its toxicity in humans. Recent study [40] proved that 8 concentrations in urine can be used to validate abstinence or measure the extent of tobacco use in persons in nicotine replacement therapy because 8 is decomposed to a much greater extent in burning tobacco then nicotine; compound 7 indole class (3.04%), from Alstonia scholaris is used in the treatment of chronic respiratory disease, like asthma and obstructive pulmonary disease, by activating  2 receptors and inhibiting NF κB [41], known to be one of the promoter of inflammation in both disease [42]. The alkaloid 4 (6.42%) is able to reduce the heart rate and blood pressure in normotensive rats [43].…”