MicroRNA-383 suppresses cell proliferation and invasion in colorectal cancer by directly targeting paired box 6

Abstract

Colorectal cancer (CRC) is the third-most prevalent cancer and the fourth‑most common cause of cancer-associated fatality worldwide. The expression and biological roles of microRNAs (miRNAs/miRs) in tumourigenesis, and their regulatory function in a number of biological processes correlated with cancer have been investigated. miR‑383 has been reported to be deregulated in several human cancer types. However, the involvement and effects of miR‑383 on CRC progression and its underlying mechanism remain unknown. Therefore, the present study aimed to examine miR‑383 expression, investigate the biological functions of miR‑383 and identify its mechanism of action in CRC cells. In the present study, miR‑383 was significantly downregulated in CRC tissues and cell lines. Low miR‑383 expression was negatively associated with tumour size, lymph node metastasis and TNM stage. Function experiments demonstrated that miR‑383 upregulation inhibited the proliferation and invasion of CRC cells. Paired box 6 (PAX6) was confirmed as a direct target of miR‑383. PAX6 was upregulated in CRC tissues and was negatively correlated with miR‑383 expression. Induced PAX6 overexpression effectively rescued the tumour‑suppressing roles of miR‑383 on CRC cell proliferation and invasion. These findings suggested that miR‑383 may act as a tumour suppressor in CRC by directly targeting PAX6 and may serve as a promising therapeutic target for CRC treatment.

Retracted Article: Circular RNA hsa_circ_0000467 modulates SGK1 to facilitate cell migration, metastasis, and EMT while repressing apoptosis in colorectal cancer by sponging miR-383-5p

“…The above results of miR-383 were in line with previous studies. 15,16 These results unraveled that hsa_circ_0000467 knockdown refrained CRC progression by sponging miR-383-5p.…”

“…Also, miR-383 was reported to be dysregulated in CRC and regulate CRC progression. 15,16 Serum/ glucocorticoid regulated kinase 1 (SGK1), located on human chromosome 6q23.2, encodes a serine/threonine protein kinase which plays imperative roles in physiological processes in our body. 17 Recent studies implied that the aberrant expression of SGK1 was also implicated in tumor progression and chemoresistance in various tumors, including CRC.…”

Combined identification of three miRNAs in serum as effective diagnostic biomarkers for HNSCC

“…MiR-383, situated in the seventh intron of the apoptosis-associated tyrosine kinase (AATK) gene, has been identified as a tumor suppressor in several cancers. In colorectal cancer (CRC), miRNA-383 was demonstrated to inhibit tumor growth and metastasis by directly targeting Paired box 6 [36]. An inhibitory effect on cell invasion and metastasis of miR-383 was also illustrated in cervical cancer via suppression of the PI3K-AKT-mTOR signaling pathway by down-regulating PARP2 [37].…”

LncRNA AK077216 is downregulated in diabetic retinopathy and inhibited the apoptosis of retinal pigment epithelial cells by downregulating miR-383

“…In a recent study Yan et al reported that miR-383 inhibited colorectal cancer by directly targeting paired box 6 [18], which is a critical player in eye development [19]. Therefore, AK077216 may regulate paired box 6 through miR-383 to participate in physiological and pathological processes in eye.…”