Molecular Cloning and Complete Nucleotide Sequence of Rice Ragged Stunt Oryzavirus Genome Segment 10.

Jiang, Yan; Suga, Haruhisa; Uyeda, Ichiro; Lee, Sang Yong; Kimura, Ikuo; Shikata, Eishiro

doi:10.3186/jjphytopath.61.189

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…Genome sequences obtained so far confirm this classification. The available information on the Oryzavirus genus has been published by Yan et al (1992Yan et al ( , 1995 and Upadhaya e t al. The available information on the Oryzavirus genus has been published by Yan et al (1992Yan et al ( , 1995 and Upadhaya e t al.…”

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confidence: 99%

The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments

et al. 1996

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An existing cDNA library of the segmented double-stranded RNA genome of maize rough dwarf virus, a plantinfecting reovirus of the genus Fifivirus was used to further characterise three genome segments. Segments $7, $8 and S10 were completely sequenced and found to consist of 1936, 1900 and 1802 base pairs, respectively. They all contained the conserved structural motifs present in all genome segments of plant-infecting reoviruses. Computer analysis indicated that the coding strands of segments $7 and S10 each contained a single large open reading frame [ORF], consisting of 591 and 558 codons, respectively, as is the norm for reovirus genome segments. Segment $8 contained two large non overlapping ORFs consisting of 347 and 209 codons, located in the 5' and 3 ~ terminal domains, respectively; an arrangement already described for segment $6. In vitro translation experiments confirmed that the gene products of gel-purified dsRNA genome segments $7 and S 10 were single polypeptides of 68 and 63 kDa, respectively. Transcription/translation of a plasmid carrying a full-length cDNA copy of segment $8 yielded two (40 and 25 kDa) polypeptides. The nucleotide sequences of segments $7 and S 10 were aligned with those of the corresponding segments of rice black-streaked dwarf virus, another member of the genus Fijivirus, and found to be almost identical.

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confidence: 99%

The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments

et al. 1996

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“…The sequenced RBSDV and MRDV genomic segments have a GjC content of 33n9-36n5 % and are AT-rich compared with other plant reoviruses, including all the rice dwarf phytoreovirus genomic segments (GjC, 41n4-48n4 % ; and rice ragged stunt oryzavirus S9 (44n9%; Uyeda et al, 1995 b) and S10 (45n4 % ; Yan et al, 1995). OSDV S7-S10 have a similar nucleotide composition and are also ATrich.…”

Section: Complete Nucleotide Sequence Of Osdv S7 S8 S9 and S10mentioning

confidence: 99%

Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome.

Isogai

Uyeda

Lindsten

1998

Journal of General Virology

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Sequence determination of full-length cDNA clones of genomic segments 7-10 (S7-S10) of oat sterile dwarf fijivirus (OSDV) revealed that the 5h and 3h ends of the plus strands of these segments had the same conserved terminal sequences, 5h AACGAAAAA and UUUUUUUAGUC 3h. These sequences are similar, but not identical, to the conserved terminal nucleotide sequences of the genomic segments of rice black streaked dwarf fijivirus (RBSDV) and maize rough dwarf fijivirus (MRDV). The coding strands of S7 and S10 each contained two large nonoverlapping open reading frames (ORFs), as do RBSDV S7 and S9, MRDV S6 and S8 and Nilaparvata lugens reovirus (NLRV ; a putative member of Fiji-

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Nucleic Acid Binding Activity of Pns6 Encoded by Genome Segment 6 of Rice Ragged Stunt Oryzavirus

Shao¹,

Lu²,

Wu³

et al. 2004

ABBS

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The ORF of genome segment 6 (S6) of rice ragged stunt oryzavirus (RRSV) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate. Pns6, the 71 kD product of S6 expressed in E. coli, was demonstrated to be a viral non-structural protein of RRSV by Western blotting. The gel mobility shift assays showed that Pns6 had nucleic acid binding activity. Pns6 could interact with single-and double-stranded forms of DNA and RNA, showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA, as demonstrated by both competition and displacement assays. The binding of Pns6 to nucleic acids is strong and sequence non-specific. By using five truncated derivatives of Pns6, it was found that the basic region from amino acid 201 to 273 of Pns6 was the unique nucleic acid binding domain. Subcellular fractionation of leaf tissues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction. The possible role of RRSV Pns6 in virus replication and assembly is discussed.

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Molecular Cloning and Complete Nucleotide Sequence of Rice Ragged Stunt Oryzavirus Genome Segment 10.

Cited by 5 publications

References 18 publications

The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments

The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments

Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome.

Nucleic Acid Binding Activity of Pns6 Encoded by Genome Segment 6 of Rice Ragged Stunt Oryzavirus

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