Optimization model for the gradient elution separation of peptide mixtures by reversed-phase high-performance liquid chromatography

Stadalius, M.A.; Gold, Harvey S.; Snyder, Lloyd R.

doi:10.1016/s0021-9673(01)96400-3

Cited by 166 publications

(49 citation statements)

References 26 publications

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“…At higher flow rates, and consequently higher shear forces, the protein tended to unfold, exposing more of its hydrophobic moiety that interacts with the reversed-phase stationary phase for increased retention. Similar results were found for carbonic anhydrase and other proteins [212].…”

Section: Effect Of Pressuresupporting

confidence: 87%

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

Fekete

Veuthey

Guillarme

2012

Journal of Pharmaceutical and Biomedical Analysis

130

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Section: Effect Of Pressuresupporting

confidence: 87%

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

Fekete

Veuthey

Guillarme

2012

Journal of Pharmaceutical and Biomedical Analysis

130

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“…To enhance interaction of hydrophilic peptides with the stationary phase the best thing to do when dealing with unknown peptide mixtures is to run water through the reversed-phase chromatographic column and inject the peptide mixture also dissolved in water or a buffer. If the peptide mixture contains a large number of peptides, the aqueous solvent has to be replaced by the organic solvent very slowly to guarantee peak separation and proper identification (Stadalius et al 1985).…”

Section: Reversed-phase Chromatographymentioning

confidence: 99%

Production and purification of recombinant hypocholesterolemic peptides

et al. 2014

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Elevated concentrations of cholesterol in plasma are associated with increased risk for heart diseases in humans. Bioactive peptides can be considered as an option to prevent or treat this condition. Currently, there are wide sources of bioactive peptides with hypocholesterolemic activities; however, most researches are focused in bioactive peptides derived from soybean and milk protein. Although there are several preparation methods for these peptides, it is a novel process to prepare bioactive peptides by genetic engineering techniques. In this review, after a general introduction on approaches and advances in bioactive peptides, recombinant strategies to generate hypocholesterolemic peptides and their purification are discussed as well as their application in food and drug design.

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“…For e > e~.r, retention of macromolecules is dominated by adsorption ( Figure 2). 6. Starting from a certain e value, apparent recovery of high molar mass samples decreases (Figures 1, 3 and 4) and, eventually, polymer species are fully retained within given column and eluent and at a given temperature.…”

mentioning

confidence: 92%

“…For example, Snyder et al [6,7] applied theoretical backgrounds of HPLC of small molecules and Armstrong and Boehm included also some pecularities of polymer solutions [8].…”

Section: Introductionmentioning

confidence: 99%

Adsorption and enthalpic partition retention mechanisms in liquid chromatography of non-charged synthetic polymers

Berek

2003

Chromatographia

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SummaryAdsorption and enthalpic partition processes in high performance liquid chromatography (HPLC) of macromolecules are qualitatively described and compared. These k, vo similar but not identical HPLC retention mechanisms result from enthalpic interactions bek, veen polymer sample and column packing, which are in different ways affected by interactions bek, veen eluent and column packing, and bek, veen eluent and sample. The understanding of basic principles of adsorption and enthalpic partition retention mechanisms in polymer HPLC may facilitate the suppression of their unwanted effects, as well as their appropriate coupling with the exclusion retention mechanism. The goal is an easy selection of suitable column packing, mobile phase, and temperature of system to attain desired separation and molecular characterization of particular complex polymer sample. The tailored coupling of enthalpic and entropic retention mechanisms with exclusion allows to either reduce or enhance separation selectivity of polymer species according to their molar masses. In the former case, macromolecules can be separated (practically) irrespectively of their molar mass in terms of other molecular characteristics, namely their chemical structure or physical architecture. On the contrary, increased selectivity of separation in terms of sample molar mass enables improving both the accuracy and speed of analysis. "Flower-like" enthalpic interactions of macromolecules in narrow pores may substantially affect the sample retention.

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Optimization model for the gradient elution separation of peptide mixtures by reversed-phase high-performance liquid chromatography

Cited by 166 publications

References 26 publications

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

New trends in reversed-phase liquid chromatographic separations of therapeutic peptides and proteins: Theory and applications

Production and purification of recombinant hypocholesterolemic peptides

Adsorption and enthalpic partition retention mechanisms in liquid chromatography of non-charged synthetic polymers

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