Ovarian stem cells are always accompanied by very small embryonic-like stem cells in adult mammalian ovary

Bhartiya, Deepa

doi:10.1186/s13048-015-0200-0

Cited by 33 publications

(35 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adult mammalian ovaries not only acts as reservoirs for oocytes but also have the presence of other valuable stem cell sources such as OSCs and VSELs. Despite having smaller sizes than OSCs, VSELs express nuclear OCT4 rather than cytoplasmic OCT4 in OSCs, which defines their pluripotent nature [101]. These pluripotent cells can be isolated from a number of other adult cell sources such as bone marrow [102], bone [103], umbilical cord blood [104], and peripheral blood [105], and they can be used to derive OLCs when induced under suitable culturing conditions.…”

Section: Very Small Embryonic-like Stem Cells (Vsels): New Heroes In mentioning

confidence: 99%

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Bharti

Jang

Lee

et al. 2020

Cells

View full text Add to dashboard Cite

In the last few decades, stem cell therapy has grown as a boon for many pathological complications including female reproductive disorders. In this review, a brief description of available strategies that are related to stem cell-based in vitro oocyte-like cell (OLC) development are given. We have tried to cover all the aspects and latest updates of the in vitro OLC developmental methodologies, marker profiling, available disease models, and in vivo efficacies, with a special focus on mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), and embryonic stem cells (ESCs) usage. The differentiation abilities of both the ovarian and non-ovarian stem cell sources under various induction conditions have shown different effects on morphological alterations, proliferation- and size-associated developments, hormonal secretions under gonadotropic stimulations, and their neo-oogenesis or folliculogenesis abilities after in vivo transplantations. The attainment of characters like oocyte-like morphology, size expansion, and meiosis initiation have been found to be major obstacles during in vitro oogenesis. A number of reports have either lacked in vivo studies or have shown their functional incapability to produce viable and healthy offspring. Though researchers have gained many valuable insights regarding in vitro gametogenesis, still there are many things to do to make stem cell-derived OLCs fully functional.

show abstract

Section: Very Small Embryonic-like Stem Cells (Vsels): New Heroes In mentioning

confidence: 99%

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Bharti

Jang

Lee

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…Cells will then gradually develop into adult cells, and the number of ES/progenitor cells is rapidly reduced, along with a decrease in potential differentiation activity. However, previous studies have suggested that embryonic-like stem cells, also called very small embryonic-like stem cells (VSELs), are present in various adult tissues, such as mammalian ovaries [3, 4], blood [5, 6], and umbilical cord blood [7, 8]. Additionally, some cells expressing ES cell-specific markers, such as Oct4, Nanog, and stage-specific embryonic antigen 4 (SSEA-4), have been identified in cord blood [9] and the adult pancreas [10].…”

Section: Introductionmentioning

confidence: 99%

Adult human pancreas-derived cells expressing stage-specific embryonic antigen 4 differentiate into Sox9-expressing and Ngn3-expressing pancreatic ducts in vivo

Lee

Kim

2016

Stem Cell Res Ther

View full text Add to dashboard Cite

BackgroundTissue-specific stem/progenitor cells are found in various adult tissues and may have the capacity for lineage-specific differentiation, facilitating applications in autologous transplantation. Stage-specific embryonic antigen 4 (SSEA-4), an early embryonic glycolipid antigen, is expressed in cells derived from adult human pancreas exocrine tissue. Here, we examined the characteristics and lineage-specific differentiation capacity of SSEA-4+ cells.MethodsHuman adult partial pancreas tissues were obtained from different donors and cultured in vitro. SSEA-4+ and CA19-9+ cells were isolated from adult human pancreas exocrine cells using magnetic-activated cell sorting, and gene expression was validated by quantitative polymerase chain reaction. To confirm in-vivo differentiation, SSEA-4+ and CA19-9+ cells were transplanted into the dorsal subcutaneous region of mice. Finally, morphological features of differentiated areas were confirmed by immunostaining and morphometric analysis.ResultsSSEA-4-expressing cells were detected in isolated pancreas exocrine cells from adult humans. These SSEA-4+ cells exhibited coexpression of CA19-9, a marker of pancreatic duct cells, but not amylase expression, as shown by immunostaining and flow cytometry. SSEA-4+ cells exhibited higher relative expression of Oct4, Nanog, Klf4, Sox2, and c-Myc mRNAs than CA19-9+ cells. Pancreatic intralobular ducts (PIDs) were generated from SSEA-4+ or CA19-9+ cells in vivo at 5 weeks after transplantation. However, newly formed PIDs from CA19-9+ cells were less abundant and showed an incomplete PID morphology. In contrast, newly formed PIDs from SSEA-4+ cells were abundant in the transplanted area and showed a crowded morphology, typical of PIDs. Sox9 and Ngn3, key transcription factors associated with pancreatic development and regeneration, were expressed in PIDs from SSEA-4+ cells.ConclusionsSSEA-4-expressing cells in the adult human pancreas may have the potential for regeneration of the pancreas and may be used as a source of stem/progenitor cells for pancreatic cell lineage-specific differentiation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-016-0422-0) contains supplementary material, which is available to authorized users.

show abstract

“…The sperm generated in vitro were not tested further since we are apprehensive of sperm obtained in a dish from VSELs after 21 days culture for clinical use. We are more enthusiastic to restore spermatogenesis by endogenous manipulation of surviving stem cells in a nonfunctional, chemoablated gonad by injecting healthy niche cells (17) and allow Mother Nature to do the rest. Sperm collected from the caudal epithelium part of epididymis by this approach were able to fertilize eggs in vitro and initiate cleavage.…”

Section: Commentarymentioning

confidence: 99%

“…The VSELs exist in adult ovary (13)(14)(15) and testis (17)(18)(19)(20) in addition to the tissue specific progenitors including ovary stem cells (OSCs) in ovary and spermatogonial stem cells (SSCs) in testis. VSELs survive oncotherapy in both mice (15,18) and human (21) gonads because of their quiescent nature whereas OSCs/SSCs and germ cells get destroyed since oncotherapy basically targets actively dividing cells.…”

Section: Commentarymentioning

confidence: 99%

Making gametes from pluripotent stem cells: embryonic stem cells or very small embryonic-like stem cells?

Bhartiya¹,

Anand²,

Patel³

2016

Stem Cell Investig.

Self Cite

View full text Add to dashboard Cite

Ovarian stem cells are always accompanied by very small embryonic-like stem cells in adult mammalian ovary

Cited by 33 publications

References 28 publications

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Adult human pancreas-derived cells expressing stage-specific embryonic antigen 4 differentiate into Sox9-expressing and Ngn3-expressing pancreatic ducts in vivo

Making gametes from pluripotent stem cells: embryonic stem cells or very small embryonic-like stem cells?

Contact Info

Product

Resources

About