“…after 72 h was observed as 0.07 µmol L −1 , 0.09 µmol L −1 and 0.11 µmol L −1 , respectively21; the EC 50 value for the alga Cylindrotheca closterium was shown to vary between 0.07 and 0.4 µmol L −1 ;42 and the MIC value for A. caldus strain BC13 was observed to be 250 mmol L −1 Cu 3. Lin and Chen43 observed EC 50 as 90 µmol L −1 for Cu to activated sludge, whereas for Escherichia coli they observed EC 50 to vary from 11.7 to 180 µmol L −1 with varying COD concentrations. Chen et al 39 observed growth inhibition for T. thiooxidans around 2300 µmol L −1 Cu.…”