Photoluminescent Hydroxylapatite: Eu3+ Doping Effect on Biological Behaviour

Abstract

Luminescent europium-doped hydroxylapatite (EuXHAp) nanomaterials were successfully obtained by co-precipitation method at low temperature. The morphological, structural and optical properties were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier Transform Infrared (FT-IR), UV-Vis and photoluminescence (PL) spectroscopy. The cytotoxicity and biocompatibility of EuXHAp were also evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay, oxidative stress assessment and fluorescent microscopy. The results reveal that the Eu3+ has successfully doped the hexagonal lattice of hydroxylapatite. By enhancing the optical features, these EuXHAp materials demonstrated superior efficiency to become fluorescent labelling materials for bioimaging applications.

Crystal Chemistry and Luminescence Properties of Eu-Doped Polycrystalline Hydroxyapatite Synthesized by Chemical Precipitation at Room Temperature

“…However, the (PO 4 ) 3− vibration bands seem slightly smoothed and less intense in the Eu-doped hydroxyapatites with respect to those of the undoped sample. This feature was previously found for other Eu-doped hydroxyapatites and was explained as the effect of the Eu incorporation into the sample [12,51].…”

Controlled Synthesis of Triangular Silver Nanoplates by Gelatin–Chitosan Mixture and the Influence of Their Shape on Antibacterial Activity

“…Nanoscale materials have been extensively studied with respect to their potential applications and synthesis procedures [1][2][3][4][5][6][7]. Among the research areas, the modification of methods for controlled synthesis of triangular silver nanoplates has attracted a lot of attention in recent years due to the widespread application of silver nanoplates in various fields, such as surface-enhanced Raman scattering (SERS) detection or manufacture of antibacterial agents [8][9][10][11][12][13].…”

Synthesis and Characterization of Photoluminescent Ce(III) and Ce(IV) Substituted Hydroxyapatite Nanomaterials by Co-Precipitation Method: Cytotoxicity and Biocompatibility Evaluation

“…The HAp and cerium-doped hydroxyapatite nanoparticles with various contents of cerium ions were synthesized by the co-precipitation method. The method was previously described [ 37 ]. The pure HAp was prepared by adding dropwise of Ca(NO 3 ) 2 ·4H 2 O aqueous solution to an appropriate amount of ammonium phosphate dibasic aqueous solution to achieve the Ca/P atomic ratio of 1.67, under magnetic stirring for 2 h, adjusting and maintaining the pH of the resulting suspension at 10.5, by adding the NH 4 OH (25%) solution.…”

“…After 24 h of seeding, cells are treated with the studied samples and then incubated for 72 h. To the obtained solution, 100 µL 1 × GSH-Glo Reagent was added, followed by incubation at 37 °C for 30 min and then 100 µL Luciferin Detection Reagent was added and incubated at 37 °C for an additional time of 15 min. The medium from the wells was well homogenized and then the plate was read on the luminometer (Microplate Luminometer Centro LB 960, Berthold, Germany) [ 37 , 38 ].…”