Physical properties of a porcine internal thoracic artery fixed with an epoxy compound

Sung, Hsing‐Wen; Hsu, Chin-Sheng; Lee, Yuag-Shein

doi:10.1016/s0142-9612(96)00081-6

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…This capability has been suggested elsewhere for altering mechanical properties of vascular grafts to match those of the host [57,58]. These studies found an increase in stiffness and tensile strength with treatment, which was not evident here despite treatment concentrations and durations being similar.…”

Section: Glutaraldehyde Treatmentsupporting

confidence: 70%

Creating a model of diseased artery damage and failure from healthy porcine aorta

Noble

Smulders

Green

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request.Creating a model of diseased artery damage and failure from healthy porcine aorta AbstractLarge quantities of diseased tissue are required in the research and development of new generations of medical devices, for example for use in physical testing. However, these are difficult to obtain. In contrast, porcine arteries are readily available as they are regarded as waste. Therefore, reliable means of creating from porcine tissue physical models of diseased human tissue that emulate well the associated mechanical changes would be valuable. To this end, we studied the effect on mechanical response of treating porcine thoracic aorta with collagenase, elastase and glutaraldehyde. The alterations in mechanical and failure properties were assessed via uniaxial tension testing. A constitutive model composed of the Gasser-Ogden-Holzapfel model, for elastic response, and a continuum damage model, for the failure, was also employed to provide a further basis for comparison [1,2]. For the concentrations used here it was found that: collagenase treated samples showed decreased fracture stress in the axial direction only; elastase treated samples showed increased fracture stress in the circumferential direction only; and glutaraldehyde samples showed no change in either direction. With respect to the proposed constitutive model, both collagenase and elastase had a strong effect on the fibre-related terms. The model more closely captured the tissue response in the circumferential direction, due to the smoother and sharper transition from damage initiation to complete failure in this direction. Finally, comparison of the results with those of tensile tests on diseased tissues suggests these treatments indeed provide a basis for creation of physical models of diseased arteries.

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Section: Glutaraldehyde Treatmentsupporting

confidence: 70%

Creating a model of diseased artery damage and failure from healthy porcine aorta

Noble

Smulders

Green

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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“…24,27 Contradictory results were reported on the use of epoxide-based crosslinkers and their effects on the stability and calcification of aortic heart valves. Myers 28 showed that ethylene glycol diglycidyl ether crosslinked valves implanted in 3-week-old Sprague-Dawley rats did not result in considerably lower calcium levels than GA-fixed controls.…”

Section: Discussionmentioning

confidence: 99%

Characterization and biocompatibility of epoxy-crosslinked dermal sheep collagens

Wachem

Zeeman

Dijkstra

et al. 1999

J. Biomed. Mater. Res.

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Dermal sheep collagen (DSC), which was crosslinked with 1, 4-butanediol diglycidyl ether (BD) by using four different conditions, was characterized and its biocompatibility was evaluated after subcutaneous implantation in rats. Crosslinking at pH 9.0 (BD90) or with successive epoxy and carbodiimide steps (BD45EN) resulted in a large increase in the shrinkage temperature (T(s)) in combination with a clear reduction in amines. Crosslinking at pH 4.5 (BD45) increased the T(s) of the material but hardly reduced the number of amines. Acylation (BD45HAc) showed the largest reduction in amines in combination with the lowest T(s). An evaluation of the implants showed that BD45, BD90, and BD45EN were biocompatible. A high influx of polymorphonuclear cells and macrophages was observed for BD45HAc, but this subsided at day 5. At week 6 the BD45 had completely degraded and BD45HAc was remarkably reduced in size, while BD45EN showed a clear size reduction of the outer DSC bundles; BD90 showed none of these features. This agreed with the observed degree of macrophage accumulation and giant cell formation. None of the materials calcified. For the purpose of soft tissue replacement, BD90 was defined as the material of choice because it combined biocompatibility, low cellular ingrowth, low biodegradation, and the absence of calcification with fibroblast ingrowth and new collagen formation.

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“…The chemical crosslinking agents include glutaraldehyde (GA), carbodiimide (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide (EDC)), epoxy compounds, six methylene diisocyanate, glycerin and alginate, etc. [ 17–21 ]; and the natural crosslinking agents include genipin (GP), nordihydroguaiaretic acid (NDGA), tannic acid and procyanidins (PC).…”

Section: Crosslinking Strategiesmentioning

confidence: 99%

“…Epoxy compounds have multiple epoxy functional groups which can react with amino, carboxyl and hydroxyl groups. Epoxy compounds have been used to preserve biological tissue materials and this new fixation technique has recently been employed to crosslink biological heart valves and vascular grafts [ 17 , 18 ]. Epoxy compounds crosslinked ECM-derived scaffolds are white, soft and no shrink, in which the collagen maintains loose and natural state [ 33 ].…”

Section: Crosslinking Strategiesmentioning

confidence: 99%

Crosslinking strategies for preparation of extracellular matrix-derived cardiovascular scaffolds

Wang

et al. 2014

Regenerative Biomaterials

158

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Heart valve and blood vessel replacement using artificial prostheses is an effective strategy for the treatment of cardiovascular disease at terminal stage. Natural extracellular matrix (ECM)-derived materials (decellularized allogeneic or xenogenic tissues) have received extensive attention as the cardiovascular scaffold. However, the bioprosthetic grafts usually far less durable and undergo calcification and progressive structural deterioration. Glutaraldehyde (GA) is a commonly used crosslinking agent for improving biocompatibility and durability of the natural scaffold materials. However, the nature ECM and GA-crosslinked materials may result in calcification and eventually lead to the transplant failure. Therefore, studies have been conducted to explore new crosslinking agents. In this review, we mainly focused on research progress of ECM-derived cardiovascular scaffolds and their crosslinking strategies.

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Physical properties of a porcine internal thoracic artery fixed with an epoxy compound

Cited by 14 publications

References 23 publications

Creating a model of diseased artery damage and failure from healthy porcine aorta

Creating a model of diseased artery damage and failure from healthy porcine aorta

Characterization and biocompatibility of epoxy-crosslinked dermal sheep collagens

Crosslinking strategies for preparation of extracellular matrix-derived cardiovascular scaffolds

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