Physiological and biochemical changes associated with cotton fiber development. VIII. Wall components

Gokani, S. J.; Thaker, Vrinda S.

doi:10.1007/s11738-000-0080-8

Cited by 8 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To estimate de-esterified pectin in transgenic cotton fibers, cell wall material (CWM) of cotton fiber was extracted and de-esterified pectic polysaccharides were extracted by using EDTA (ethylenediamine tetraacetic acid) as a chelating agent (Gokani and Thaker 2000). Color development procedure was adapted from the phenol sulfuric acid method (Dubois et al 1956).…”

Section: Estimation Of De-esterified Pectinmentioning

confidence: 99%

The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton

Wang

Guo

et al. 2009

Plant Mol Biol

View full text Add to dashboard Cite

Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. Subsequently, the corresponding PEL genes were isolated from four different cotton species and characterized. In vitro enzyme assays indicated that GhPEL really exhibited cleavage-activity against de-esterified pectin. The temporal-spatial expression analyses revealed that the GhPEL gene was preferentially expressed in fibers at 10 days-post anthesis (DPA). Antisense GhPEL transgenic cotton plants were generated by Agrobacterium-mediated transformation. Six homozygous lines, each with one or two copies of the transgene inserted as determined by southern blot analysis of the NPTII gene, were selected for further functional analysis. The GhPEL expression during fiber elongation in these transgenic lines was significantly suppressed in various degrees. Furthermore, the reduction of GhPEL enzymatic activity by decreasing GhPEL transcripts severely affected the degradation of de-esterified pectin in primary cell walls of transgenic cotton fibers, which consequently blocked cell wall loosening in early fiber development. Ultimately, the fiber elongation of all these transgenic lines was repressed. These results suggested that GhPEL may play an important role in the process of normal fiber elongation in cotton.

show abstract

Section: Estimation Of De-esterified Pectinmentioning

confidence: 99%

The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton

Wang

Guo

et al. 2009

Plant Mol Biol

View full text Add to dashboard Cite

show abstract

“…Previous reports mainly concentrated on final fibre quality; however, studies on how drought stress influences the developmental progression of the fibre are limited. The cotton fibre is a unicellular trichome of the seed coat, and fibre development occurs in four phases: initiation, elongation, secondary thickening and maturation (Gokani & Thaker, ). The elongation phase overlaps with the secondary wall thickening phase and a substantial portion of secondary wary wall thickening can occur before the elongation phase is over (Schubert, Benedict, Berlin, & Kohel, ).…”

Section: Introductionmentioning

confidence: 99%

Drought effects on cotton (Gossypium hirsutum L.) fibre quality and fibre sucrose metabolism during the flowering and boll‐formation period

Gao

Snider

Bai

et al. 2020

J Agronomy Crop Science

View full text Add to dashboard Cite

The cotton (Gossypium hirsutum L.) crop is an indeterminate species that is most sensitive to drought stress during the flowering and boll‐formation stage of development. To evaluate the effect of water deficit on fibre quality and carbon metabolism at different fruiting branches (FB), multiple controlled experiments were conducted. The cotton cultivar Siza 3 was used under three soil relative water content (SRWC): control (CK, SRWC (75 ± 5) %), moderate drought stress (MDS, SRWC (60 ± 5) %) and severe drought stress (SDS, SRWC (45 ± 5) %) treatment, imposed at the beginning of the flowering of FB6–7. Fibre quality was significantly affected by drought level and fruiting branch location on the plant. Compared to the CK, under MDS and SDS, sucrose content and sucrose synthase (SuSy) activity decreased prior to 24 days post‐anthesis (DPA; the fibre elongation phase), which caused low fibre turgor pressure. This decreased the rate of fibre elongation and final fibre length. Moreover, from 24 to 38 DPA (during fibre thickening), low fibre sucrose content led to insufficient UDP‐glucose (UDPG; uridine diphosphate glucose) for cellulose synthesis. Furthermore, more of the existing UDPG was allocated to the synthesis of callose instead of cellulose, which resulted in lower cellulose accumulation and decreased fibre strength. The magnitude of the drought stress effect on fibre qualities, as well as the formation process, was strongly influenced by fruiting branch position, where differences were most pronounced for fruiting branches further away from the base of the plant.

show abstract

“…Mature cotton fiber is composed of more than 90% cellulose, about 3% pectic and hemicellulosic polysaccharides (Tokumoto et al ). Both the amounts and molecular sizes of pectin and hemicellulose polysaccharides show substantial changes during the cotton fiber development (Gokani and Thaker , Tokumoto et al ). Thus, biosynthesis of hemicellulosic polysaccharides is required for both primary wall formation and secondary wall thickening stages in normal fiber development.…”

Section: Introductionmentioning

confidence: 99%

Two cotton fiber‐associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development

Huang

Qin

et al. 2014

Physiologia Plantarum

View full text Add to dashboard Cite

Xylan is the major hemicellulosic constituent in dicot secondary cell walls. Cell wall composition of cotton fiber changes dynamically throughout development. Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development. However, none of the genes encoding glycosyltransferases (GTs) responsible for synthesizing xylan have been isolated and characterized in cotton fiber. In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively. We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl. Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively. Furthermore, we show that overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis results in increased xylan content. We also show that overexpression of GhGT43A1 or GhGT43C1 leads to more cellulose deposition. These findings suggest that GhGT43A1 and GhGT43C1 likely participate in xylan synthesis during fiber development.

show abstract

Physiological and biochemical changes associated with cotton fiber development. VIII. Wall components

Cited by 8 publications

References 23 publications

The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton

The essential role of GhPEL gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton

Drought effects on cotton (Gossypium hirsutum L.) fibre quality and fibre sucrose metabolism during the flowering and boll‐formation period

Two cotton fiber‐associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development

Contact Info

Product

Resources

About