Phytochemical analysis of Calotropis procera with antimicrobial activity investigation

Morsy, Nagy; Sherif, Emad A. Al; Abdel-rassol, Tayseer M.A.

doi:10.3233/mgc-160206

Cited by 17 publications

(7 citation statements)

References 18 publications

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“…The phytochemical content of Calotropis procera observed in this study agrees with other reports which showed the presence of phenols, alkaloids, tannins, flavonoids and saponins [6]. Similar values for the total content of some of the phytochemicals have also been reported; Banerjee et al [21] reported a total phenol content of 40.7 (mg GAE g −1 ) while Najar et al [22] recorded a total flavonoid content of 36.75 (mg GAE g −1 ).…”

Section: Qualitative and Quantitative Phytochemical Analysissupporting

confidence: 90%

Green synthesis of magnetite nanoparticles using calotropis procera leaf extract and evaluation of its antimicrobial activity

Kalu¹,

Egwim²,

Jigam³

et al. 2022

Nano Ex.

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In this study, magnetite nanoparticles were successfully synthesized using Calotropis procera aqueous leaf extract. The effect of the whole extract and some of its phytochemicals on the particle size distribution was determined using Dynamic light scattering (DLS) while other characterizations such as UV–vis spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) spectroscopy and Scanning electron microscopy (SEM) were carried out. The antimicrobial activity against selected microorganisms was also studied using the agar well diffusion method. The leaf extract showed the presence of phenols, flavonoids, saponins, alkaloids and tannins. The magnetite nanoparticle synthesized using the leaf extract (Calotropis procera/Fe3O4) had an average particle size of 11.1nm with a PDI of 0.142 while the phenolic, flavonoid and saponin extracts of Calotropis procera leafs produced magnetites with average particle sizes of 62.83nm, 68.02nm and 134nm respectively. The UV-vis spectra displayed the characteristic plasmon resonance peak at 420nm, the FTIR plots highlighted important functional groups including peaks between 600cm-1 and 400cm-1 confirming the formation of iron oxide while the SEM micrographs showed the nanoparticles were mainly irregular in shape with areas of agglomeration. Calotropis procera/Fe3O4 displayed significant anti-microbial activity against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumonia, Aspergillus niger and Fusarium oxysporum while no activity was recorded against Escherichia coli. The study indicated that Calotropis procera leaf extract was suitable for the green synthesis of magnetite with properties that suggest that it could be potentially applied as antimicrobial nanofillers.

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Section: Qualitative and Quantitative Phytochemical Analysissupporting

confidence: 90%

Green synthesis of magnetite nanoparticles using calotropis procera leaf extract and evaluation of its antimicrobial activity

Kalu¹,

Egwim²,

Jigam³

et al. 2022

Nano Ex.

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“…Flavonoid was also presence in both Calotropis procera leaf and fruit extract at a concentration of 53.50 and 50.23 mg/100g. The study by Morsy et al (2016) only detected flavonoid in the leaves of C. procera. Flavonoids has the potential to suppress microbial, allergic, inflammatory and cancer activities, while on the other hand, tannins do possess cytotoxic and anti-tumor activities (Joshi et al, 2013).…”

Section: Plant Science Journalmentioning

confidence: 90%

Calotropis procera Leaves and Fruits Phytochemical and GCMS Analysis and Evaluation of their Methanolic Extracts Antimicrobial effect on Isolated Bacteria

OLU

YUSUF²,

DANTANKO³

et al. 2022

PSJ

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This study was conducted to investigate the bioactive and chemical components, ethnobotany and pharmacological activities of Calotropis procera leaves and fruits with a focus on its antimicrobial effect on isolated bacteria. To prepare the plant extract, methanol was used as the solvent, for evaluation of the bioactive compounds and their concentration in the plant extracts hence, qualitative and quantitative phytochemical analysis was conducted respectively. The GCMS analysis was conducted for further determination of the chemical composition of the plant parts. The antimicrobial impacts of the plant leaves and fruits methanolic extracts were carried out against assay organisms which were bacterial group and they include Standard and clinical strains of Bacillus subtilis ATCC 6633, Bacillus subtilis clinical, Staphylococcus aureus β-haemolytic strain and clinical, Staphylococcus aureus, Salmonella typhi clinical, Klebsiella pneumonia clinical, Escherichia coli and Proteus sp. Clinical strain. This study showed the presences of some phytochemical compounds such as tannin, alkaloid, saponin, flavonoid etc. in C. procera leaves and fruits at varying concentration. The GCMS analysis revealed that Pentadecanoic acid and n-Hexadecanoic acid were detected in both leaves and fruits of C. procera extracts. Furthermore, methanolic extract of the plant leaves had antibacterial effect potency towards Bacillus subtilis ATCC 6633, while the fruit extract had no antibacterial effect against the assay organism.

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“…Sporulation and germination were also affected by the aqueous extract. This antifungal activity is due to different types of secondary metabolites, such as glycocides, alkaloids and calotropin (Morsy et al, 2016). C. procera has a type of protein in its composition that facilitates the permeabilization of the membrane of spores and hyphae of fungi, facilitating its deterioration (Ranjit et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Antagonism of Plant Pathogens by Calotropis procera

Cardoso¹,

Martins²,

Souza³

et al. 2021

JAS

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Phomopsis sojae and Sclerotinia sclerotiorum are responsible for stem and pod dryness and white mold in soybean. These pathologies directly affect the quality of seeds/grains and compromise the entire plant. The use of extracts from different plants has been the subject of research for the control of several phytopathogens. Calotropis procera is among botanical species that synthesize efficient compounds for biocontrol. In this context, the aim of this study was to evaluate the in vitro effect of C. procera aqueous extract on P. sojae and S. sclerotiorum. The experiment was carried out in completely randomized blocks in a 2 × 5 factorial scheme (two fungi and five extract concentrations 0%, 5%, 10%, 15% and 20%) with 4 replicates. C. procera aqueous extract concentrations were added to Petri dishes containing PDA. After 48 hours, the mycelial growth rate was evaluated. After seven days of incubation, the fungal colony area, sporulation, and germination of P. sojae and S. sclerotiorum were evaluated. There was significant interaction between fungi × extract concentrations (p < 0.05) for all variables analyzed. The mycelial growth rate of P. sojae was lower than that of S. sclerotiorum. The diameter of the P. sojae fungal colony was smaller than that of S. sclerotiorum when concentrations of 5%, 10% and 15% were used. As the extract concentration increased, fungi sporulation and germination reduced.

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Phytochemical analysis of Calotropis procera with antimicrobial activity investigation

Cited by 17 publications

References 18 publications

Green synthesis of magnetite nanoparticles using calotropis procera leaf extract and evaluation of its antimicrobial activity

Green synthesis of magnetite nanoparticles using calotropis procera leaf extract and evaluation of its antimicrobial activity

Calotropis procera Leaves and Fruits Phytochemical and GCMS Analysis and Evaluation of their Methanolic Extracts Antimicrobial effect on Isolated Bacteria

Antagonism of Plant Pathogens by Calotropis procera

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