Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle

Khasanah, Himmatul; Gunawan, Asep; Priyanto, Rudy; Ulum, Mokhamad Fakhrul; Jakaria, Jakaria

doi:10.5398/medpet.2016.39.2.95

Cited by 21 publications

(24 citation statements)

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“…Another study also stated that there was a significant finding of the incidence of the C  T polymorphism in 5'UTR CYP11B1 gene in Pakistan Sahiwal cattle breed (Manzoor et al, 2013). Khasanah et al (2016) stated that myostatin (MSTN) promoter gene was polymorphic in Bali cattle and associated with intramuscular fat percentage. From the studies above, no study was conducted in Bali cattle.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Sihite

Priyanto

Jakaria

2019

Trop. Anim. Sci. J.

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The aim of this study was to identify the variation of 5'UTR CAPN1 gene and its association to growth traits in Bali cattle. DNA samples were obtained from 80 heads of Bali cattle originated from BPTU-HPT Denpasar. The average of Bali cattle age was 784 days (631 days-1098 days). Bali cattle were divided into 3 age groups namely, the first group (1.5 years to 2 years), the second group (2 years to 2.5 years), and the third group (2.5 years to 3 years). The observed growth traits were birth weight (kg), live weights (kg), average daily gain (kg), body length (cm), chest depth (cm), withers height (cm), hip height (cm), and heart girth (cm). Polymorphism identification of 5'UTR CAPN1 gene was conducted by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with BglII as the restriction enzyme. Growth traits data association were analyzed using the General Linear Model (GLM) analysis. The 5'UTR CAPN1 gene|BglII was polymorphic in Bali cattle (GG, GT, and TT). Genotype frequencies for Bali cattle were 0.30 (GG), 0.66 (GT), and 0.04 (TT). The allele frequencies of G and T allele were 0.63 and 0.37, respectively. The G allele was the most frequent allele and GT genotype was the most frequent genotype among the cattle. The CAPN1|BglII had a significant effect (p<0.05) on growth traits in Bali cattle. Animal carrier of GG genotype had higher live weight and average daily gain than those with GT genotype, while the lowest values were associated with TT genotype.

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Section: Introductionmentioning

confidence: 99%

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Sihite

Priyanto

Jakaria

2019

Trop. Anim. Sci. J.

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“…Gen MSTN merupakan anggota dari superfamily transforming growth factorβ (TGF-β) yang berfungsi sebagai inhibitor (negative regulator) pertumbuhan jaringan otot rangka. Peningkatan massa otot rangka akibat mutasi gen MSTN juga ditemukan pada sapi Belgian Blue dan Piedmontese (Buske et al 2011), sapi Korea (Han et al 2012) dan sapi Bali (Khasanah et al 2016). Hal ini menunjukkan bahwa gen MSTN berperan penting dalam mengatur pertumbuhan ternak dan proses pembentukan massa otot rangka.…”

Section: Pathway Gen Myostatinunclassified

“…Polimorfisme gen MSTN diketahui memiliki peran penting dalam proses hipertrofi, peningkatan massa otot dan pertumbuhan sel. Banyak penelitian telah melaporkan asosiasi positif antara polimorfisme gen MSTN dengan sifat pertumbuhan dan sifat karkas pada beberapa spesies ternak, termasuk sapi (Zhang et al 2007, Fiems, 2012, Khasanah et al 2016, Haruna et al 2020, kambing (An et al 2011;Batubara 2017), domba (Haren et al 2020), ayam (Zhang et al 2020) dan itik (Lu et al 2011). Pada Tabel 1 ditampilkan polimorfisme gen MSTN pada beberapa bangsa sapi.…”

Section: Polmorfisme Gen Myostatin Dan Asosiasinya Terhadap Sifat Pertumbuhan Dan Karkasunclassified

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Myostatin Gene as a Genetic Marker for Growth and Carcass Traits in Beef Cattle

Prihandini¹,

Hariyono

Tribudi

2021

WARTAZOA

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<p>Growth and carcass traits are of economic importances in livestock breeding, because they affect the profitability of animal production. The phenotypic expression of these traits is controlled by multiple genes (polygenes), such as myostatin (MSTN) gene. This paper aims to discuss the expression, polymorphism and potential application of MSTN gene as a marker-assisted selection (MAS) for growth and carcass traits in beef cattle based on data from published studies. MSTN gene or known as growth and differentiation factor 8 (GDF8) is a member of the transforming growth factor-β (TGF-β) superfamily, which acts as a negative regulator of skeletal muscle mass deposition. Several published studies showed that mutations in the MSTN gene can inhibit the activation of myostatin, which leads to an increased muscle mass (hypertrophy). Several <em>MSTN</em> gene polymorphisms were reported to be associated with growth and carcass traits in local cattle in several countries, including Indonesia, namely Bali cattle. Based on several assumptions: 1) there is MTSN gene polymorphisms in a population, 2) there is a significant association between MSTN gene polymorphisms and growth and carcass traits, as reported in several beef cattle populations and 3) those cattle with superior genotype have better growth performances, we expect that there will be improvement in growth performances in the future if those cattle are selected. Understanding MSTN gene polymorphisms would be useful to make strategies for the genetic improvement for growth and carcass traits of local cattle.</p>

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“…Hasil penelitian-penelitian sebelumnya menunjukkan bahwa pada sapi Bali (Bos javanicus) memiliki perbedaan posisi SNP dengan bangsa sapi Bos taurus maupun bangsa sapi Bos indicus. Beberapa penelitian yang menemukan posisi SNP baru pada sapi Bali seperti pada gen calpain-1 (CAPN1) (Pratiwi et al 2016), gen myogenic factor 5 (Myf5), gen myostatin (MSTN) (Khasanah et al, 2016), gen stearoyl-CoA denaturase (SCD) (Alwiyah et al 2016), gen thyroglobulin 5 (TG5) (Anwar et al 2017), dan gen endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) (Sutikno et al, 2018).…”

Section: Frekuensi Genotipe Dan Alel Snp G125550a>t Gen Fto Pada Sapunclassified

Identifikasi Keragaman Gen FTO Pada Bangsa Sapi Potong Indonesia

Sutikno

Priyanto²,

Sumantri³

et al. 2019

jitro

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ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.

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Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle

Cited by 21 publications

References 28 publications

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Polymorphism and Association of 5’UTR CAPN1 Gene with Growth Traits in Bali Cattle by PCR-RFLP

Myostatin Gene as a Genetic Marker for Growth and Carcass Traits in Beef Cattle

Identifikasi Keragaman Gen FTO Pada Bangsa Sapi Potong Indonesia

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