Healthcare professionals are faced with long-term work, excessive workload, time pressure, difficult or complex tasks, insufficient rest breaks and physically bad work conditions. For this reason, it may be difficult to increase the job satisfaction of healthcare workers and to keep their performance at a high level. In this study, it was aimed to investigate the mediating role of job satisfaction in the effect of perception of excessive workload on employee performance. Workload Surplus Scale, Employee Performance Scale and Job Satisfaction Scale were used in data collection. The research was applied to 324 healthcare workers working in a public hospital. AMOS and SPSS 21 statistical programs were used to analyze the data obtained in the study. As a result of the research, it was seen that job satisfaction has a mediating role in the effect of the perception of workload on employee performance. In addition, it was tested whether the perceptions of employees regarding excessive workload, employee performance and job satisfaction differ according to the socio-demographic variable. In the analysis, it was determined that the perceptions of employees' burden, employee performance and job satisfaction differ according to socio-demographic variables. In line with the findings obtained; Business owners and managers are required to allocate a reasonable workload to employees in order to increase the performance and job satisfaction of their employees.