Preparation of Glucosamine from Exoskeleton of Shrimp and Predicting Production Yield by Response Surface Methodology

Mojarrad, Javid Shahbazi; Nemati, Mahboob; Valizadeh, Hadi; Ansarin, Masood; Bourbour, Samira

doi:10.1021/jf062983a

Cited by 65 publications

(51 citation statements)

References 11 publications

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“…Chitin is a biopolymer composed of N-acetyl-d-glucosamine, which can be found in exoskeletons of invertebrate marine creatures such as oysters, crabs, or shrimps. Glucosamine is usually prepared from chitin by hydrolysis using strong mineral acid (sulphuric or hydrochloric) (Mojarrad et al 2007). During this reaction, chitin is deacetylated and depolymerised to glucosamine salt of the acid used (Figures 1a-c).…”

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confidence: 99%

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Václavíková¹,

Kvasnička²

2013

Czech J. Food Sci.

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Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech J. Food Sci., 31: 55-65.Glucosamine and chondroitin sulfate, components of normal cartilage, are used as ingredients in dietary supplements intended to treat osteoarthritis and/or to support joint health. Of concern is the documented lack of quality in many of the marketed products. We present here a capillary isotachophoretic method for the determination of glucosamine and chondroitin sulfate in dietary supplements. Cationic analysis of glucosamine was performed with a leading electrolyte consisting of 10mM NH 4 OH + 20mM acetic acid. As the leading electrolyte for anionic analysis of chondroitin sulphate, a mixture of 5mM HCl + 10mM glycylglycine + 0.05% of 2-hydroxyethylcellulose was used. The solution of 10mM citric acid served as the terminating electrolyte for both glucosamine and chondroitin sulfate analyses. The analytes were detected by conductivity and UV detectors. The characteristics of the method,, i.e., linearity, accuracy, repeatability, and quantitation limit, were evaluated. On a set of 35 samples of marketed dietary supplements did we prove that the capillary isotachophoresis is a suitable method for the routine analysis of glucosamine and chondroitin sulfate.

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confidence: 99%

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Václavíková¹,

Kvasnička²

2013

Czech J. Food Sci.

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“…By an acidic hydrolysis, chitin can be depolymerized and deacetylated to produce GlcN. 17,18 Thus, chitin can be determined from the amount of GlcN residue in the hydrolysate. Usually, HPLC techniques have been employed for the GlcN assay, [19][20][21] although this application presents some difficulties.…”

Section: Introductionmentioning

confidence: 99%

Determination of Chitin Based on the Colorimetric Assay of Glucosamine in Acidic Hydrolysate

et al. 2016

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A colorimetric method for the glucosamine (GlcN) assay was applied for the determination of chitin, which can be hydrolyzed to produce GlcN. A 10-mg sample was mixed with 10 mL of a 5 mol/L HCl aqueous solution, and the mixture was kept at 100 C for 12 h. Under these conditions, chitin was completely depolymerized and deacetylated to produce GlcN, even when the sample was a crab shell. A 20-μL aliquot of the hydrolysate was mixed with 20 μL of a 5 mol/L NaOH aqueous solution and 200 μL of a 50 mmol/L Na2SiO3, 600 mmol/L Na2MoO4, 1.5 mol/L CH3COOH and 30% (v/v) dimethyl sulfoxide solution. The mixture was kept at 70 C for 30 min. In the mixture, GlcN reduced the Mo(VI) species to form a blue molybdosilicate anion, which gave an absorbance maximum at around 750 nm. Since N-acetylglucosamine and chitin oligosaccharides could not render the reaction mixture blue, GlcN in the hydrolysate could be assayed colorimetrically with high selectivity. When a standard chitin sample was examined, the GlcN concentration in the hydrolysate was determined to be 0.97 ± 0.02 g/L (as hydrochloride salt), indicating that the sample contained 10.0 ± 0.2 mg chitin (as an N-acetylglucosamine homopolymer). Calcium cation, amino acids, and proteins did not interfere with the GlcN assay. Thus, the proposed method was successfully applied to determine chitin in a crab shell sample.

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“…Rhizopus sp, Mucor sp, Gongronella butleri) [2][3][4][5][6]. GlcN and GlcNAc can also be obtained from the exoskeleton of the crustaceans [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Productions of GlcN using chemical processes are usually carried out by chemical and or enzyme hydrolysis, wherein the energy expenditure leads to increased production cost and also need longer time [7,9]. Several studies have reported the production of GlcN from microbial fermentations.…”

Section: Introductionmentioning

confidence: 99%

Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

Sitanggang

Wang

2009

Biotechnol Bioproc E

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A rapid method for the determination of fungal glucosamine (GlcN) from ^ëéÉêÖáääìë=ëé BCRC 31742 was developed. The hydrochlorination process using microwave effectively reduced reaction time needed for GlcN analysis. The analytical method consisted of two steps: (1) hydrochlorination of fungal cells and (2) derivatization process. Fungal GlcN hydrochloride was reacted with 1-napthyl isothiocyanate (1-NITC) as the derivatizing agent to enhance the sensitivity of GlcN and so to achieve high resolution. This method was specific for quantification of GlcN hydrochloride at the wavelength of 230 nm. The standard deviation and relative error of the analytical results were less than 5%. By using microwave heating, the reaction time of hydrochlorination process was shortened from 24 h to 3 min. Thus, the overall time needed for analyzing GlcN from fungal sources was reduced from 5 h (thermal method) to 2 h (microwave method). © KSBB hÉóïçêÇëW=ãáÅêçï~îÉI=ÑìåÖ~ä=ÖäìÅçë~ãáåÉI=ãáÅêçÄá~äI=âáåÉíáÅëI=ÅÜêçã~íçÖê~éÜóI=çéíáãáò~íáçå= = = = =

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Preparation of Glucosamine from Exoskeleton of Shrimp and Predicting Production Yield by Response Surface Methodology

Cited by 65 publications

References 11 publications

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements

Determination of Chitin Based on the Colorimetric Assay of Glucosamine in Acidic Hydrolysate

Determination of fungal glucosamine using HPLC with 1-napthyl isothiocyanate derivatization and microwave heating

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