Preparative separation of lithospermic acid B from Salvia miltiorrhiza by polyamide resin and preparative high-performance liquid chromatography

Guo, Yongxue; Zhou, Lina; Li, T.; Wang, L.H.

doi:10.1016/j.chroma.2011.05.048

Cited by 17 publications

(11 citation statements)

References 21 publications

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“…3S, Supporting Information). The hydrogen binding affinity of polyamide resin was orthogonal to size exclusion and reversed-phase [11,15] [17]. Therefore, we speculated that these four compounds have trihydroxyl-flavone backbones (such as kaempferol, datiscetin, or fisetin), and the glycosyl group was substituted at C-3 [18][19][20].…”

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confidence: 99%

Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry

et al. 2014

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The chemical composition of Drynaria fortunei, a traditional Chinese herbal medicine, is very complicated. In order to separate these chemicals to obtain their structural information, an orthogonal sample enrichment procedure was established. The ethyl acetate extract of D. fortunei was pre-separated by Sephadex LH-20 × polyamide columns to yield 15 fractions. These fractions were analyzed successively using a reversed-phase Agilent Zorbax SB-C18 column, coupled with diode array detection and electrospray ionization tandem mass spectrometry. The method reduced co-elution and enriched minor compounds on the basis of their chemical features. A total of 369 compounds were detected by LC/MSn, compared to less than 50 compounds without pre-separation. The pretreatment facilitated the analytical separation of flavonoids, proanthocyanidins, triterpenoids, phenolic acids, and lignans in D. fortunei, and allowed a comprehensive chemical profiling of these constituents. This method could be applied to other multicomponent herbal extracts.

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confidence: 99%

Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry

et al. 2014

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“…In this way, we ensure our conclusions can be used to establish a QS for herbs that is suitable for the clinical use and has guaranteed the clinical efficacy. Although there is a limitation of the strategy for its dependence on the separation of constituents, the combination of multiple techniques such as preparative high performance liquid chromatography and high speed counter current chromatography could be used to remove the targeted constituent353637. Meanwhile, with the development of further researches on the constituent separation of herbal medicines, we believe that the strategy will also be improved better.…”

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confidence: 99%

Promotion of quality standard of herbal medicine by constituent removing and adding

Yan

Xiong

et al. 2014

Sci Rep

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To identify major active constituents and measure their levels in a typical medicinal herb–Rhizoma coptidis, we applied the concept of removing and adding, taking inspiration from functional genetic methods. As this herb has bacteriostatic properties and is used to treat bacterial diarrhea, we examined the effects of individual constituents (berberine, palmatine, coptisine, epiberberine, jateorrhizine and columbamine) on the growth of Shigella dysenteriae with microcalorimetry. The removing and adding procedures revealed that berberine and coptisine were the main antibacterial constituents of R. coptidis, with bacteriostatic activities of 54.10% and 39.75%, respectively. The relative levels of berberine and coptisine in R. coptidis were 8.08%–31.92% and 4.05%–14.45%, respectively. On the basis of whole effect, the method of constituents removing and adding, coupled with a bioassay, is a useful strategy to identify the active constituents and measure their levels in herbal medicines, which may provide reference to other natural products.

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“…HPLC separations were usually carried out on all kinds of reversed-phase C18 (RP C18) or some C8 [91] analytical columns so as to obtain optimized analysis. Due to the acidity of phenolic acids, acidic modifiers were often added to suppress their ionization and to attain the suitable peak shapes and separation.…”

Section: Methodsmentioning

confidence: 99%

Chemical Analysis of the Herbal Medicine Salviae miltiorrhizae Radix et Rhizoma (Danshen)

et al. 2016

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Radix Salviae miltiorrhizae et Rhizoma, known as Danshen in China, is one of the most popular traditional Chinese medicines. Recently, there has been increasing scientific attention on Danshen for its remarkable bioactivities, such as promoting blood circulation, removing blood stasis, and clearing away heat. This review summarized the advances in chemical analysis of Danshen and its preparations since 2009. Representative established methods were reviewed, including spectroscopy, thin layer chromatography, gas chromatography, liquid chromatography (LC), liquid chromatography-mass spectrometry (LC-MS), capillary electrophoresis, electrochemistry, and bioanalysis. Especially the analysis of polysaccharides in Danshen was discussed for the first time. Some proposals were also put forward to benefit quality control of Danshen.

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Preparative separation of lithospermic acid B from Salvia miltiorrhiza by polyamide resin and preparative high-performance liquid chromatography

Cited by 17 publications

References 21 publications

Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry

Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry

Promotion of quality standard of herbal medicine by constituent removing and adding

Chemical Analysis of the Herbal Medicine Salviae miltiorrhizae Radix et Rhizoma (Danshen)

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