Prevalence of species and genotypes of Cryptosporidium found in 1–2-year-old dairy cattle in the eastern United States

Fayer, Ronald; Santı́n, Mónica; Trout, James M.; Greiner, Ellis C.

doi:10.1016/j.vetpar.2005.08.003

Cited by 222 publications

(179 citation statements)

References 33 publications

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“…The Cryptosporidium oocysts detected in buffalo calf fecal smears in this study were morphologically similar to those of C. parvum described in calves in many previous studies (Fall et al, 2003;Fayer et al, 2006;Hassanain et al, 2011;Randhawa et al, 2012b). Identification of the Cryptosporidium species depended upon the oocyst morphology and measurements (Fayer et al, 2000).…”

Section: Discussionsupporting

confidence: 89%

“…Synchronized analysis of the obtained morphological simultaneous with molecular criteria of Cryptosporidium buffalo's oocytes in the present study could prove that the isolates were C. parvum. Theoretically, it was known that C. parvum is infectious to many mammalian hosts worldwide (Fayer et al, 2006;Santin and Zarlenga, 2009). Calves were the major recognized reservoirs for C. parvum (Caccio et al, 2000;Warda et al, 2002;Condoleo et al, 2007;Paul et al, 2008;Helmy et al, 2013;Mahfouz et al, 2014) with strong relation between C. parvum infection and diarrhea among Egyptian buffalo calves (Warda et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abdelrahma¹,

Megeed²,

Hammam³

et al. 2015

Research J. of Parasitology

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Cryptosporidium an apicomplexan parasite has the ability to induce diarrhea in bovines, goats, pigs, dogs and cats worldwide. In this study, buffalo calves fecal samples were examined after staining their smears with Modified Ziehl-Neelsen Stain (MZN). Ileal sections were examined for the detection of pathological changes. Further molecular characterization was done using nested PCR amplification and partial sequence analysis. The detected oocysts were morphologically similar to Cryptosporidium parvum. Light microscopic examination of Cryptosporidium infected ileal Tissue Section (TS) stained with H and E revealed the presence of altered mucosal architecture with congestion of blood vessels, infiltration, sloughing and complete erosion of epithelial cells and shortening, blunting, stunting and atrophy of the intestinal villi. Molecular characterization gave PCR amplicons of 18S SSU rRNA gene products approximately at 823 bp. Sequences proved specified generalized relatedness with 21 species of Cryptosporidium but the nucleotide homogeneity percentage was insufficient to designate species or genotypes. Further bioinformatics analysis showed that resulting Cryptosporidium isolates had the closest match with three isolates. It was implied that the Cryptosporidium isolates is mostly like Cryptosporidium parvum (JX237832.1) previously isolated from buffaloes in Ismailia province.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abdelrahma¹,

Megeed²,

Hammam³

et al. 2015

Research J. of Parasitology

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“…Indeed, C. bovis, a species recently described (Fayer et al, 2005), is reported to be not associated with signs of disease (Santin et al, 2004). C. bovis has been reported as preferentially infect post-weaned calves (> 3 months of age) but has been also found in sheep (Fayer et al, 2006;Santin et al, 2007). Very recently, Santin et al (2007) identified also C. bovis in lambs.…”

Section: Discussionmentioning

confidence: 98%

Prevalence ofCryptosporidiumspp. (Eucoccidiorida: Cryptosporiidae) in seven species of farm animals in Tunisia

Soltane¹,

Guyot

Dei‐Cas

et al. 2007

Parasite

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Summary :1,001 faecal samples were obtained from 89 sheep (lambs and adult), 184 goats, 190 horses, 178 rabbits, 110 camels, 200 broiler chicken and 50 turkeys housed in farms from different localities in Tunisia. All samples were analysed for Cryptosporidium oocysts by microscopic examination of smears stained by modified Ziehl Neelsen technique. The parasite was detected in ten lambs and adult sheep (11.2 %) and nine broiler chicken (4.5 %). Molecular characterization, performed in four animals, identified C. bovis in three lambs and C. meleagridis in one broiler chicken. This work is the first report on Cryptosporidium in farm animals in Tunisia. Résumé

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“…The difference could be due to a vast number of factors such as breed, age, management, environment, and season as well as diagnostic method [5,7,13] . The low prevalence could also be caused by spot fecal sampling instead of serial sampling, which may result in underestimation because of intermittent oocyst excretion [9,11] .…”

Section: Discussionmentioning

confidence: 99%

“…The agent is responsible for about 85% of cryptosporidiosis in preweaned calves but only 1% of the disease in postweaned calves and 1-2 year old heifers [6,9,10] . Among the other bovine species, C. bovis and C. ryanae were detected mainly in weaned calves, and C. andersoni in yearlings and adult cattle [9,11,12] . While C. bovis and C. ryanae are considered non-zoonotic, C. andersoni has recently been reported in few research involving humans in England [13] .…”

Section: Prevalence Of Cryptosporidiosis and Molecular Characterizatimentioning

confidence: 99%

Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

Güven¹,

Avcıoğlu²,

Balkaya³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryThis study was conducted to determine the prevalence of cryptosporidiosis and to identify Cryptosporidium species found in preweaned calves, in Erzurum, Turkey. Fecal samples were collected from 307 calves up to one month old from 5 dairy farms. Genomic DNA was obtained by DNA extraction (QIAamp DNA Stool kit). The prevalence of cryptosporidiosis was determined based on identification through a nested PCR protocol to amplify fragments of the Cryptosporidium SSU rRNA gene. 3.9% of calves were positive for Cryptosporidium. Calves that were subjected to traditional herd management, were female, aged 2 weeks, and had watery feces were affected by the disease at a greater incidence than those were subjected to planned herd surveillance program, were males, were older than 3 weeks, and had firm feces. DNA sequence analysis of the SSU rRNA gene on all of the PCR positive samples ascertained that C. parvum was the only species present. Further studies should be performed comprehensive fecal analysis for other causative agents for association Cryptosporidium species in calf diarrhea and mortality resulting in economic loss in the region. Keywords: Calf, Cryptosporidium, Nested-PCR, SSU rRNA, Erzurum Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı veCryptosporidium Türlerinin Moleküler Karekterizasyonu ÖzetBu çalışma, Erzurum yöresindeki sütten kesim öncesi dönemdeki buzağılarda cryptosporidiosisin prevalansının ve Cryptosporidium türlerinin moleküler karekterizasyonunun ortaya konması amacıyla yapılmıştır. Bu amaçla beş süt işletmesinden, bir aydan küçük 307 buzağının dışkı örnekleri toplanmış ve DNA ekstraksiyonu (QIAamp DNA Stool kit) yapılarak genomik DNA elde edilmiştir. Nested PCR protokolü ile Cryptosporidium SSU rRNA gen bölgesinin kısmi amplifikasyonu yapılmış ve cryptosporidiosis prevalansı %3.9 olarak belirlenmiştir. Geleneksel yöntemlerle yetiştirilen, dişi, 2 haftalık yaşta ve sulu dışkıya sahip buzağıların modern işletmelerde yetiştirilen, erkek, 3 haftadan büyük ve katı kıvamlı dışkıya sahip olanlara göre hastalıktan daha çok etkilendiği saptanmıştır. PCR pozitif örneklerin SSU rRNA gen bölgesi hedef alınarak yapılan DNA dizi analizleri sonuçlarına göre C. parvum'un hayvanlarda bulunan tek tür olduğu anlaşılmıştır. Sonuç olarak, yörede ekonomik kayıplara neden olan buzağı ishalleri ve ölümlerinde Cryptosporidium türleri ile diğer hastalık etkenlerinin etkileşimlerinin ortaya konması amacıyla daha kapsamlı çalışmaların yapılmasının gerekliliği sonucuna varılmıştır.

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Prevalence of species and genotypes of Cryptosporidium found in 1–2-year-old dairy cattle in the eastern United States

Cited by 222 publications

References 33 publications

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Prevalence ofCryptosporidiumspp. (Eucoccidiorida: Cryptosporiidae) in seven species of farm animals in Tunisia

Erzurum Yöresinde Buzağılarda Cryptosporidiosisin Prevalansı ve Cryptosporidium Türlerinin Moleküler Karekterizasyonu

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