Prognostic role of microRNA polymorphisms in patients with advanced esophageal squamous cell carcinoma receiving platinum-based chemotherapy

Wu, Chaohui; Li, Minjie; Hu, Chao; Duan, Hongbing

doi:10.1007/s00280-013-2364-x

Cited by 40 publications

(33 citation statements)

References 36 publications

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“…A growing body of evidence suggests that single nucleotide polymorphisms (SNPs) in pre-miRNA genes may interfere with the mature processing or the degradation of miRNA by compromising the secondary structure and causing a reduction of the amount of mature miRNA, which attenuates or abolishes the inhibition of the target genes [14][15][16]. Furthermore, functional SNPs located in pre-miRNA may be associated with altered susceptibility to numerous diseases, including cancer, reproductive disorders and degenerative diseases [17,18]. MiR-125a is significantly down regulated in the nucleus pulposus cells of the patients diagnosed with IDD [19], but the downstream mediators and signaling pathways have not been identified.…”

Section: Introductionmentioning

confidence: 99%

MiR-125a Rs12976445 Polymorphism is Associated with the Apoptosis Status of Nucleus Pulposus Cells and the Risk of Intervertebral Disc Degeneration

Feng

Zang

et al. 2016

Cell Physiol Biochem

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Background: Spinal degenerative diseases are a major health problem and social burden worldwide. Intervertebral disc degeneration (IDD) is the pathological basis of spinal degenerative diseases and is characterized by loss of nucleus pulposus cells due to excessive apoptosis caused by various factors. MicroRNAs (miRNAs) have been reported to be functionally involved in the control of apoptosis. Methods: computational analysis and luciferase assay were used to identify the target of miR-125a, and cell culture, transfection were used to confirm such relationship. Sequencing was used to determine the genotype of each participant. Results: We confirmed the previous report that the presence of the minor allele (T) of rs12976445 polymorphism significantly downregulated the expression level of miR-125a in nucleus pulposus cells, leading to less efficient inhibition of its target gene. We also validated TP53INP1 as a target of miR-125a in nucleus pulposus cells using a dual luciferase reporter system, and the transfection of miR-125a significantly reduced the expression of TP53INP1. The expression level of TP53INP1 was significantly lower in nucleus pulposus cells genotyped as CT or TT than in those genotyped as CC, and the apoptosis rate was consistently lower in the CC group than in the nucleus pulposus cells collected from individuals carrying at least one minor allele of rs12976445 polymorphism. To study the association between rs12976445 polymorphism and the risk of IDD, we enrolled 242 patients diagnosed with IDD and 278 normal controls, and significant differences were noted regarding the genotype distribution of rs12976445 between the IDD and the control groups (OR = 2.69, 95% C.I. = 1.88-3.83, p < 0.0001). In summary, rs12976445 polymorphism is significantly associated with the risk of IDD in the Chinese population. Conclusion: The present study indicated that miR-125a is a promising potential target for patients with IDD in clinical practice.

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Section: Introductionmentioning

confidence: 99%

MiR-125a Rs12976445 Polymorphism is Associated with the Apoptosis Status of Nucleus Pulposus Cells and the Risk of Intervertebral Disc Degeneration

Feng

Zang

et al. 2016

Cell Physiol Biochem

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“…Among these 20 miRNAs, 13 miRNAs, such as miR-16 [8], miR-93 [9], miR-200c [10], miR-25-3p [11], miR-34a [12], miR-181a [13], miR-107 [14], miR-103a [15], miR-151a [16], miR-149 [17], miR-550a [18], miR-378a [19], and miR-30c [20], have been associated with the malignant potential of esophageal cancer.…”

Section: Discussionmentioning

confidence: 99%

The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma

et al. 2016

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BackgroundDespite advances in radical esophagectomies and adjuvant therapy, the postoperative prognosis in esophageal squamous cell carcinoma (ESCC) patients remains poor. The aim of this study was to identify a molecular signature to predict postoperative favorable outcomes in patients with ESCC.MethodsAs a training data set, total RNA was extracted from formalin-fixed paraffin-embedded samples of surgically removed specimens from 19 ESCC patients who underwent curative esophagectomy. The expression of microRNA (miRNA) was detected using a miRNA oligo chip on which 885 genes were mounted. As a validation data set, we obtained frozen samples of surgically resected tumors from 12 independent ESCC patients and the expression of miR-574-3p was detected by quantitative real-time PCR.ResultsOur microarray analysis in the training set patients identified three miRNAs (miR-574-3p, miR-106b, and miR-1303) and five miRNAs (miR-1203, miR-1909, miR-204, miR-371-3p, miR-886-3p) which were differentially expressed between the patients with (n = 14) and without (n = 5) postoperative tumor relapse (p < 0.01 and p < 0.05, respectively). Higher expression of miR-574-3p, which showed the most significant association with non-relapse (p = 0.001), was associated with favorable overall survival (p = 0.016). Real-time PCR experiments on the validation set patients confirmed that higher expression of miR-574-3p was associated with non-tumor relapse (p = 0.029) and better overall survival (p = 0.004).ConclusionsOur results suggest that the aberrant expression of the miRNAs identified in this study plays key roles in the progression of ESCC. miR-574-3p was suggested to have a tumor suppressor effect, and thus, to be a predictor of postoperative outcome in patients with ESCC.Electronic supplementary materialThe online version of this article (doi:10.1186/s12957-016-0985-3) contains supplementary material, which is available to authorized users.

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“…Therefore, a function SNP in miRNA genes may affect many signaling pathways by altering the expression levels of thousands of genes (Wu et al 2014). Previous studies have shown that SNPs in miRNA genes are associated with the susceptibility to many disease, including cancer (Omrani et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Since single nucleotide polymorphisms (SNPs) located in pre-miR genes may directly affect the miRNA maturation and expression, and the binding to target mRNAs, and subsequently affect the expression of target genes (Wu et al 2014). Therefore, a function SNP in miRNA genes may affect many signaling pathways by altering the expression levels of thousands of genes (Wu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…There are more than 2,500 mature human miRNA sequences in the miRBase database (release 20.0) (Kozomara and Griffiths-Jones 2011). It has been suggested that each miRNA potentially regulates hundreds of target genes (Wu et al 2014), and miRNAs are thus likely involved in almost all biological processes, such as cell proliferation, cell proliferation, differentiation, apoptosis and stress resistance (Yu et al 2013;Wu et al 2014). It is not surprising that abnormal levels of miRNAs have been implicated in many human disease, including cancer ) and cardiovascular diseases (Condorelli et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Association of microRNA Polymorphisms with the Risk of Myocardial Infarction in a Chinese Population

Chen

Hong

Chen

et al. 2014

Tohoku J. Exp. Med.

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MicroRNAs (miRNAs) are involved in the regulation of a variety of biological processes, such as inflammation. Dysregulation of miRNAs have been implicated in many human disease, including cardiovascular diseases. Polymorphisms in miRNA genes may affect the miRNA biogenesis and function, and thus cause changes in the expression of thousands of genes. The aim of this study was to examine whether miRNA polymorphisms (miR-146a rs2910164, miR-149 rs71428439, miR-196a2 rs11614913, miR-218 rs11134527, and miR-499 rs3746444) contribute to the risk for the development of myocardial infarction (MI). Five miRNA polymorphisms were genotyped in a total of 1808 subjects composed of 919 MI patients and 889 control individuals. The GG genotype of rs3746444 was found to be associated with a significantly increased risk of MI (recessive model, adjusted OR = 1.710, 95% CI: 1.058-2.763, P = 0.029). Although the CC genotype of rs2910164 significantly increased the risk of MI under dominant and additive models (P < 0.05), this difference disappeared after adjustment for age, sex, blood pressure, triglycerides, total cholesterol, HDL, LDL and diabetes. In addition, when rs3746444 and rs2910164 were evaluated together by the number of putative high-risk alleles, we found an increased risk of MI for subjects carrying 3-4 risk alleles (3-4 risk alleles vs. 0-1 risk allele, adjusted OR = 1.580, 95% CI: 1.069-2336, P = 0.022; 3-4 risk alleles vs. 0-2 risk allele, adjusted OR = 1.513, 95% CI: 1.031-2.219, P = 0.034). These findings indicate that miR-499 rs3746444 and miR-146a rs2910164 may represent novel markers of MI susceptibility.

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Prognostic role of microRNA polymorphisms in patients with advanced esophageal squamous cell carcinoma receiving platinum-based chemotherapy

Abstract: Taken together, these findings indicate that miRNA polymorphisms may predict prognosis in advanced ESCC patients receiving platinum-based chemotherapy.

Cited by 40 publications

References 36 publications

MiR-125a Rs12976445 Polymorphism is Associated with the Apoptosis Status of Nucleus Pulposus Cells and the Risk of Intervertebral Disc Degeneration

MiR-125a Rs12976445 Polymorphism is Associated with the Apoptosis Status of Nucleus Pulposus Cells and the Risk of Intervertebral Disc Degeneration

The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma

Association of microRNA Polymorphisms with the Risk of Myocardial Infarction in a Chinese Population

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