Protective effect of apigenin magnesium complex on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and inflammatory responses in rat hepatic stellate cells

Pan, Xuwang; Shao, Yi; Wang, Fugen; Cai, Zhen; Liu, Shourong; Xi, Jianjun; He, Ruoyu; Zhao, Yanmei; Zhuang, Ran

doi:10.1080/13880209.2020.1772840

Cited by 25 publications

(17 citation statements)

References 34 publications

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“…In this study, it was found that AP could reduce the ROS content in frozen semen and increase the expression of various antioxidant enzymes. It was also observed in previous studies that treatment with AP attenuated the reduction in superoxide dismutase activity and glutathione levels and reduced the levels of reactive oxygen species and malondialdehyde in rat hepatic stellate cells [ 27 ]. These findings are similar to the results of this study.…”

Section: Discussionsupporting

confidence: 55%

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Wang

Yuan

et al. 2021

Animals

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The purpose of this study was to determine the effects of apigenin and astragalus polysaccharides on the cryopreservation of bovine semen. Apigenin, astragalus polysaccharides, or their combination were added to a frozen diluent of bovine semen. Afterwards, Computer Assisted Semen Analysis (CASA), membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-Px, MDA, and ROS detection were conducted. The results showed that adding 0.2 mmol/L AP or 0.5 mg/mL APS could improve the quality of frozen sperm. Compared to 0.2 mmol/L AP alone, the combination of 0.2 mmol/L AP and 0.3 mg/mL APS significantly increased the total motility (TM), average path distance (DAP), straight line distance (DSL), average path velocity (VAP), curvilinear velocity (VCL), wobble (WOB), and sperm CAT and SOD levels (p < 0.05), while reducing the ROS and MDA levels (p < 0.05). These results indicated that the addition of 0.2 mmol/L AP or 0.5 mg/mL APS alone has a protective effect on the freezing of bovine semen. Compared to the addition of 0.2 mmol/L AP, a combination of 0.2 mmol/L AP and 0.3 mg/mL APS could further improve the quality of frozen semen.

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Section: Discussionsupporting

confidence: 55%

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Wang

Yuan

et al. 2021

Animals

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“…Studies have demonstrated that the metabolism of alcohol results in increased levels of cytochrome P450 family 2 subfamily E member 1 in hepatocytes and increased levels of nicotinamide adenine dinucleotide phosphate oxidase in hepatocytes, hepatic Kupffer cells, and infiltrated inflammatory cells, mainly contributing to the generation of ROS (De Minicis and Brenner, 2008;Abdelmegeed et al, 2017). GSH is a crucial factor in the endogenous protective system for eliminating ROS, and the lack of GSH in hepatocytes may disturb the antioxidant defense system, leading to ROS accumulation (Pan et al, 2020). In this study, we used DHE and NA-8 probes to visualize the level and distribution of ROS and GSH in the livers of live larvae.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation

Lai

Tan

et al. 2021

Front. Pharmacol.

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Alcoholic liver disease (ALD) has become a heavy burden on health worldwide. Ginsenoside Rb1 (GRb1), extracted from Panax quinquefolium L., has protective effects on many diseases, but the effect and mechanisms of GRb1 on ALD remain unknown. This study aimed to investigate the protective effects of GRb1 on ALD and to discover the potential mechanisms. Zebrafish larvae were exposed to 350 mM ethanol for 32 h to establish a model of acute alcoholic liver injury, and the larvae were then treated with 6.25, 12.5, or 25 μM GRb1 for 48 h. The human hepatocyte cell line was stimulated by 100 mM ethanol and meanwhile incubated with 6.25, 12.5, and 25 μM GRb1 for 24 h. The lipid changes were detected by Oil Red O staining, Nile Red staining, and triglyceride determination. The antioxidant capacity was assessed by fluorescent probes in vivo, and the expression levels of inflammatory cytokines were detected by immunohistochemistry, immunofluorescence, and quantitative real-time PCR. The results showed that GRb1 alleviated lipid deposition in hepatocytes at an optimal concentration of 12.5 μM in vivo. GRb1 reversed the reactive oxygen species accumulation caused by alcohol consumption and partially restored the level of glutathione. Furthermore, GRb1 ameliorated liver inflammation by inhibiting neutrophil infiltration in the liver parenchyma and downregulating the expression of nuclear factor-kappa B pathway-associated proinflammatory cytokines, including tumor necrosis factor-α and interleukin-1β. This study revealed that GRb1 has a protective effect on alcohol-induced liver injury due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions. These findings suggest that GRb1 may be a promising candidate against ALD.

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“…The ability of APG treatment to reduce the oxidative stress induced by Pb in testis and thyroid glands may be due to the antioxidant activity of APG which is mainly determined by three hydroxyl groups at its 4', 5, 7 position and double bond at C2 and C3 (Pan et al, 2020).…”

Section: Effect Of Apg On Oxidative Stress Markers In Testis and Thyroid Gland Of Rats Treated With Lead Acetatementioning

confidence: 99%

Apigenin Ameliorates Lead Acetate induced Hyperlipidemia, Hypothyroidism and Hypogonadism in Male Rats

Algefare

Sedky

Alfwuaires

et al. 2021

Preprint

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Lead (Pb) is an environmental pollutant and has detrimental effects on human health. Apigenin (APG) is a flavonoid that have antioxidant, anti-inflammatory, antiallergic, and cardioprotective effects and is presented as treatment of some diseases. The aim of the present study was to evaluate the probable protective effect of APG against Pb-induced toxicity in rats. Adult male rats were given either Pb (as lead acetate; 20 mg/kg) alone or in combination with APG (20 mg/kg) daily for 4 weeks by intraperitoneally injection (i.p). At the end of the experimental period, Pb accumulation, lipid profile, thyroid, and testicular function alterations were assessed. In addition, histopathological changes in the testis were assessed.Results revealed that Pb treatment significantly increased Pb concentrations in blood, thyroid gland, and in testis of rats. Further, the blood levels of hormones related to thyroid gland and testis were altered in Pb-treated rats. In parallel, low sperm count and sperm motility, increased sperm abnormalities, and marked pathological changes in testis were observed. On the contrary, the treatment with both Pb and APG recorded amelioration of the deleterious effects of Pb, involving attenuation of changes in lipid profile, thyroidal and testicular hormonal levels, sperm parameters and pathological changes in Pb treated rat’s testis. In conclusion, it appears that dietary APG can ameliorate lead acetate induced hyperlipidemia, hypothyroidism and hypogonadism in male rats.

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Protective effect of apigenin magnesium complex on H₂O₂-induced oxidative stress and inflammatory responses in rat hepatic stellate cells

Abstract: Zhuang (2020) Protective effect of apigenin magnesium complex on H 2 O 2 -induced oxidative stress and inflammatory responses in rat hepatic stellate cells,

Cited by 25 publications

References 34 publications

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation

Apigenin Ameliorates Lead Acetate induced Hyperlipidemia, Hypothyroidism and Hypogonadism in Male Rats

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Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells

Abstract: Zhuang (2020) Protective effect of apigenin magnesium complex on H 2 O 2 -induced oxidative stress and inflammatory responses in rat hepatic stellate cells,

Cited by 25 publications

References 34 publications

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation

Apigenin Ameliorates Lead Acetate induced Hyperlipidemia, Hypothyroidism and Hypogonadism in Male Rats

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Protective effect of apigenin magnesium complex on H₂O₂-induced oxidative stress and inflammatory responses in rat hepatic stellate cells