Rapid characterization of Ziziphi Spinosae Semen by UPLC/Qtof MS with novel informatics platform and its application in evaluation of two seeds from Ziziphus species

Zhang, Fengxiang; Li, Min; Qiao, Lin; Yao, Zhihong; Chang, Li Chien; Shen, Xiu‐Yu; Wang, Yu; Yu, Kate; Yao, Xin‐Sheng; Dai, Yi

doi:10.1016/j.jpba.2016.01.047

Cited by 92 publications

(82 citation statements)

References 22 publications

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“…In addition, MS ions of catechin oligomers and emodin glycosides mainly contributed to differentiating PMC from PMR samples. The compounds that might have served as potential chemical markers were identified as follows: prodelphinidin (3), procyanidin B (4 and 5), catechin (6), trimer of epi/catechin (7), epicatechin (10), galloyl-procyanidin (11), Z-2,3,5,4' -tetrahydroxystilbene-2-O-β-D-glucoside (13), E-2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (14), torachrysone-O-glucoside (19), emodin-O-glucoside (20), citreorosein (21), emodin-(acetyl)-O-glucoside (22), questinol/fallacinol (33). Among them, some constituents such as peak 7 were only found in PMC samples during identification.…”

Section: Screening the Differentiating Components Of Pmc And Pmrmentioning

confidence: 99%

Rapid Characterization of the major chemical constituents from Polygoni Multiflori Caulis by liquid chromatography tandem mass spectrometry and comparative analysis with Polygoni Multiflori Radix

Wang

Shang

et al. 2017

J. Sep. Sci.

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Polygoni Multiflori Caulis is a traditional Chinese medicine that has been used for a long time to treat sleep disorders. However, the multiple chemical composition analysis has not been reported. In this study, a simple, rapid and effective ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established to characterize the components of Polygoni Multiflori Caulis. In addition, a chemical comparative analysis was performed with Polygoni Multiflori Radix, another traditional Chinese medicine from the same plant, through multivariate statistical analysis and semi-quantitative analysis to screen the difference in chemical ingredients between these two herbal medicines from same medicinal plant. A total of 33 peaks were detected within 25 min, and 28 of them were identified or tentatively characterized by comparing the retention time and mass spectrometry data. Based on the results, 12 characteristic components were screened out by multivariate statistical analysis, and their content change trends were compared by semi-quantitative analysis.

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Section: Screening the Differentiating Components Of Pmc And Pmrmentioning

confidence: 99%

Rapid Characterization of the major chemical constituents from Polygoni Multiflori Caulis by liquid chromatography tandem mass spectrometry and comparative analysis with Polygoni Multiflori Radix

Wang

Shang

et al. 2017

J. Sep. Sci.

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“…The MS 2 ion at m/z 287 suggested a kaempferol moiety based on its MS 3 ions at m/z 241 and 258. Therefore, it was identified as kaempferol‐3‐ O ‐glucoside (Ren et al ., ; Zhang et al ., ). The fragment ion at m/z 303 was further fragmented to characteristic ions at m/z 285, 257 and 229 (Fig.…”

Section: Resultsmentioning

confidence: 97%

Analysis of solvent effects on polyphenols profile, antiproliferative and antioxidant activities of mulberry (Morus alba L.) extracts

Zhang

et al. 2017

Int J of Food Sci Tech

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Summary A systematic study about the solvent effect on the mulberry polyphenol (MP) profile, antioxidant and antiproliferation capacities against human hepatoma HepG2 cells was conducted. Results indicated that MP profiles showed significant differences as a function of solvents including AA/W (acetic acid/water), MeOH/AA/W (methanol/ acetic acid/water), EtOH/AA/W (ethanol/ acetic acid/water) and ME2CO/AA/W (acetone/ acetic acid/water). Among the solvents, EtOH/AA/W took advantage in obtaining MP with the highest yield (49.81 mg GAE g−1 dw), and cellular antioxidant capacity in the PBS no‐wash protocol (63.2 μmolQE/100 g), as well as low cell cytotoxicity (≥50.0 mg mL−1). ME2CO/AA/W was the best choice for extracting MP with the most various compositions (two phenolic acids, four anthocyanins and four flavonols), greatest potential in inhibiting proliferation of HepG2 cells (EC50 = 28.2 mg mL−1) and highest intracellular antioxidant activity (38.0 μmolQE/100 g). Results provide baseline information for efficient solvent extraction to obtain MP that is promising as natural antioxidants and antitumor agents.

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“…It was reported that ZSS contained a variety of complex chemical ingredients, such as flavonoids, saponins, alkaloids and three triterpenoids, etc [27][28][29][30][31]. Thus, the MS was operated under both positive and negative ion modes to screen all these relative compounds in ZSS as comprehensively as possible.…”

Section: Experimental Conditions Optimizationmentioning

confidence: 99%

Development of an analytical strategy to identify and classify the global chemical constituents of Ziziphi Spinosae Semen by using UHPLC with quadrupole time‐of‐flight mass spectrometry combined with multiple data‐processing approaches

Zhu

Xiao

Pei

et al. 2018

J of Separation Science

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According to traditional Chinese medical theory, Ziziphi Spinosae Semen needs to be stir-fried before clinical application for its sedative-hypnotic effect enhancement. A rapid and comprehensive analysis strategy of ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry and multiple data analysis platforms was developed for the efficient and sensitive identification of components in crude and parched Ziziphi Spinosae Semen to explore the composition changes that happen during the stir-frying process. Both positive and negative ion modes were applied for mass spectrometry detection, and 40 components were identified from crude and parched Ziziphi Spinosae Semen, respectively. Principal component analysis and t-test were applied to find differences between crude and parched Ziziphi Spinosae Semen. As a result, Ziziphi Spinosae Semen samples could be clearly divided into two groups according to their processing methods, and 19 key markers that contributed to the classification significantly (P < 0.05) were found. This kind of change in contents of components might be responsible for the recommended clinical application of parched Ziziphi Spinosae Semen.

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Rapid characterization of Ziziphi Spinosae Semen by UPLC/Qtof MS with novel informatics platform and its application in evaluation of two seeds from Ziziphus species

Cited by 92 publications

References 22 publications

Rapid Characterization of the major chemical constituents from Polygoni Multiflori Caulis by liquid chromatography tandem mass spectrometry and comparative analysis with Polygoni Multiflori Radix

Rapid Characterization of the major chemical constituents from Polygoni Multiflori Caulis by liquid chromatography tandem mass spectrometry and comparative analysis with Polygoni Multiflori Radix

Analysis of solvent effects on polyphenols profile, antiproliferative and antioxidant activities of mulberry (Morus alba L.) extracts

Development of an analytical strategy to identify and classify the global chemical constituents of Ziziphi Spinosae Semen by using UHPLC with quadrupole time‐of‐flight mass spectrometry combined with multiple data‐processing approaches

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