Reduced expression of biomarkers associated with the implantation window in women with endometriosis

Abstract

Objective-To evaluate the expression of biomarkers of implantation, Glycodelin A (GdA), Osteopontin (OPN), Lysophosphatidic acid receptor 3 (LPA3), and HOXA10, in eutopic endometrium of women with and without endometriosis.Design-Prospective observational study. Setting-Clinical Research Center.Patient(s)-Twenty-four women with endometriosis and 23 healthy volunteers of similar age. Intervention(s)-Secretory phase endometrial biopsy.Main Outcome Measure(s)-Expression of immunohistochemical staining intensity and localization of GdA, OPN, LPA3 and HOXA10 in eutopic endometrium. Result(s)-EndometrialGdA expression was significantly reduced in patients after cycle day 22. The endometrium from women with endometriosis also showed decreased expression of OPN in the late secretory phase and LPA3 and HOXA10 expression in the mid-and late secretory phases. Conclusion(s)-The decreased expression of these four biomarkers of implantation may indicate impaired endometrial receptivity in endometriosis patients, providing one explanation for the subfertility observed even in women with few pelvic implants. As many of these markers are progesterone-dependent, these findings suggest the possibility of reduced endometrial progesterone action in this population.

Intrafollicular interleukin-8, interleukin-12, and adrenomedullin are the promising prognostic markers of oocyte and embryo quality in women with endometriosis

“…Impairment in the receptive status of the endometrium in women with endometriosis is well evidenced [45,46]. It appears that both the endometrium and the oocyte/embryo are affected in endometriosis, and it remains to be unraveled as to which factor is more important in adversely affecting the pregnancy outcome.…”

Molecular mechanisms of membrane interaction at implantation

“…A number of studies have reported that the expression of epithelial avb3 is associated with the window of implantation and that the endometrium is devoid of this particular integrin in certain cases of infertility (Lessey et al, 1992;Castelbaum et al, 1994), endometriosis (Lessey et al, 1994;Lessey, 2002;Wei et al, 2009;Casals et al, 2012), and idiopathic infertility (Lessey et al, 1995). The level of integrin b3 mRNA on day 21 has been suggested as a possible indicator with which to predict success rates following ART, while avb3 has been considered as a clinical biomarker for human implantation (Elnashar and Aboul-Enein, 2004).…”

Modern approaches to endometrial receptivity assessment (a review)

“…-интерлейкина-1, -6, -11 (IL-I, -6, -11) [45,46]; -лейкемияингибирующего фактора (LIF) [47]; -сосудисто-эндотелиального фактора роста (VEGF) [48]; -гепаринсвязывающего эпидермального фак-тора роста (ГС-ЭФР) [49]; -матриксных металлопротеиназ (ММР) и их тканевых ингибиторов (ТIMP) [50]; -интегринов [46,47]; -селектинов [51,52]; -гликоделина A [53]; -кадгеринов [45]; -муцинов [45]; -простагландинов [54]; -белка Ki-67 [55,56]. Перечисленные молекулярные маркеры могут определяться в крови, секрете эндометрия или в са-мой ткани эндометрия, полученной при его биопсии.…”