Regulation of m6A RNA Methylation and Its Effect on Myogenic Differentiation in Murine Myoblasts

Chen, J. N.; Chen, Y.; Wei, Yuanhang; Raza, Muhammad Ali; Zou, Qin; Xi, X. Y.; Zhu, Li; Tang, Guoqing; Jiang, Yanzhi; Li, X. W.

doi:10.1134/s002689331903004x

Cited by 21 publications

(13 citation statements)

References 28 publications

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“…Myoblasts are a crucial research model to study skeletal muscle proliferation and differentiation, in which myoblasts form multinucleated myotubules under induced conditions ( 14 ). Kudou and Chen confirmed that METTL3 regulates myoblast differentiation and m6A modifies MyoD expression ( 15 , 16 ). m6A-modified genes in the skeletal muscle of wild boar and in Changbai and Rongchang pigs are interspecific, and these genes tare mainly enriched in transcription factor binding and cell metabolism ( 17 ).…”

Section: Introductionmentioning

confidence: 90%

Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Bao

et al. 2022

Front. Vet. Sci.

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N6-methyladenine (m6A) RNA undergoes epigenetic modification, which is the most extensive intermediate chemical modification in mRNA. Although this modification occurs in all living organisms, it is the most widely studied among mammals. However, to date, no study has investigated the m6A transcriptome-wide map of yak and its potential biological functions in muscle development. In this study, the differences of m6A methylation and gene expression in yak muscle development belonging to three age groups, namely 3 years (group A), 6 months (group M), and 90-day-old fetuses (group E), were determined by using methylated RNA immunoprecipitation sequencing (MeRIP-Seq) and RNA sequencing (RNA-Seq). In these three groups, a total of 6,278 (A), 9,298 (E), and 9,584 (M) m6A peaks were identified, with average densities between 1.02 and 2.01. m6A peaks were mostly enriched in the stop codon, 3′ untranslated region (UTR) region, and inner long exon region with consensus motifs of UGACA. In all the three stages, the m6A peak enrichment level was significantly negatively correlated with mRNA abundance (Pearson's correlation coefficient r = −0.22 to −0.32, p < 10−16). The functional enrichment of genes consistently modified by m6A methylation, particularly those genes that regulate cell differentiation as well as muscle growth and development, was observed at all three stages. Moreover, m6A abundance was negatively associated with gene expression levels, indicating that m6A might play a vital role in modulating gene expression during yak muscle development. This comprehensive map thus provides a solid foundation for determining the potential functional role of m6A RNA modification in yak muscle growth.

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Section: Introductionmentioning

confidence: 90%

Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Bao

et al. 2022

Front. Vet. Sci.

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“…Using mouse C2C12 myoblasts and muscle-specific adult stem cells (MuSCs) as models, recent studies have suggested that RNA m 6 A methylation plays an important role in myoblast proliferation and differentiation in vitro (Figure 3). The m 6 A modifications are closely linked to genes associated with transcriptional regulation during proliferation, and positively regulate myogenic differentiation in C12C12 skeletal myoblasts [103,104].…”

Section: The Function and Mechanism Of M 6 A During Myogenesis In Vitromentioning

confidence: 99%

Regulation of RNA N⁶-methyladenosine modification and its emerging roles in skeletal muscle development

Pei

Zhou

et al. 2021

Int. J. Biol. Sci.

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“…Based on the involvement of m6A in the regulation of mouse brain development, fat formation, and other tissue development processes, we speculate that m6A is also involved in the regulation of skeletal muscle development ( Zhao et al, 2014 ; Ma et al, 2018 ; Wang et al, 2018 ). Studies have shown that the regulation of METTL3 gene expression and regulation of m6A levels in myoblasts affect the differentiation process of myocytes and the expression of key regulatory genes ( Kudou et al, 2017 ; Chen et al, 2019 ). This shows that m6A is involved in the regulation of muscle cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Global Landscape of m6A Methylation of Differently Expressed Genes in Muscle Tissue of Liaoyu White Cattle and Simmental Cattle

Yunlong

Dong

et al. 2022

Front. Cell Dev. Biol.

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Liaoyu white cattle (LYWC) is a local breed in Liaoning Province, China. It has the advantages of grow quickly, high slaughter ratew, high meat quality and strong anti-stress ability. N6 methyladenosine (m6A) is a methylation modification of N6 position of RNA adenine, which is an important modification mechanism affecting physiological phenomena. In this study, we used the longissimus dorsi muscle of LYWC and SIMC for m6A-seq and RNA-seq high-throughput sequencing, and identified the key genes involved in muscle growth and m6A modification development by bioinformatics analysis. There were 31532 m6A peaks in the whole genome of LYWC and 47217 m6A peaks in the whole genome of SIMC. Compared with Simmental cattle group, LYWC group had 17,351 differentially expressed genes: 10,697 genes were up-regulated, 6,654 genes were down regulated, 620 differentially expressed genes were significant, while 16,731 differentially expressed genes were not significant. Among the 620 significantly differentially expressed genes, 295 genes were up-regulated and 325 genes were down regulated. In order to explore the relationship between m6A and mRNA expression in the muscles of LYWC and SIMC, the combined analysis of MeRIP-seq and RNA-seq revealed that 316 genes were m6A modified with mRNA expression. To identify differentially methylated genes related to muscle growth, four related genes were selected for quantitative verification in LYWC and SIMC. GO enrichment and KEGG analysis showed that the differentially expressed genes modified by m6A are mainly involved in skeletal muscle contraction, steroid biosynthesis process, redox process, PPAR pathway and fatty acid metabolism, and galactose metabolism. These results provide a theoretical basis for further research on the role of m6A in muscle growth and development.

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Regulation of m6A RNA Methylation and Its Effect on Myogenic Differentiation in Murine Myoblasts

Cited by 21 publications

References 28 publications

Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Regulation of RNA N⁶-methyladenosine modification and its emerging roles in skeletal muscle development

Global Landscape of m6A Methylation of Differently Expressed Genes in Muscle Tissue of Liaoyu White Cattle and Simmental Cattle

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Regulation of m6A RNA Methylation and Its Effect on Myogenic Differentiation in Murine Myoblasts

Cited by 21 publications

References 28 publications

Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Regulatory Role of N6-Methyladenosine in Longissimus Dorsi Development in Yak

Regulation of RNA N6-methyladenosine modification and its emerging roles in skeletal muscle development

Global Landscape of m6A Methylation of Differently Expressed Genes in Muscle Tissue of Liaoyu White Cattle and Simmental Cattle

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Regulation of RNA N⁶-methyladenosine modification and its emerging roles in skeletal muscle development