Repair process of surgical defects filled with autogenous bone grafts in tibiae of diabetic rats

Abstract

From a biological standpoint, the best material for reconstruction of bone defects is the autogenous bone graft. However, as tissue healing is affected under diabetic conditions, major changes might take place in the revascularization, incorporation, replacement and remodeling phases of the grafted area. The purpose of this study was to assess the bone healing process in surgical wounds prepared in tibiae of diabetic rats and filled with autogenous bone. Forty male rats (Rattus norvegicus albinus, Wistar) were randomly assigned to receive an endovenous injection (penile vein) of either citrate buffer solution (Group 1 - control; n=20) or streptozotocin dissolved in citrate buffer solution (35 mg/kg) to induce diabetes (Group 2 - diabetic; n=20). After determination of glycemia, the animals were anesthetized and the anterolateral regions of the tibiae of both limbs were shaved, antisepsis was performed and longitudinal incisions were made in each limb. The tibiae were exposed and two 2-mm-diameter surgical cavities were prepared: one in the right limb, filled with particulate autogenous bone and the other in the left limb, filled with blood clot. The animals were euthanized at 10 and 30 postoperative days. The anatomic pieces were obtained, submitted to laboratory processing and sections were stained by hematoxylin and eosin and Masson's Trichrome for histomorphologic and histometric analyses. In both groups, the wounds filled with autogenous bone graft showed better results than those filled with blood clot. The control group showed higher new bone formation in wounds filled with autogenous bone graft at 30 days than the diabetic group, but without statistical significance. It may be concluded that, in general, the new bone formation occurred with autogenous graft was quantitatively similar between control and diabetic groups and qualitatively better in the control group.

Effects on Bone Repair of Osteotomy With Drills or With Erbium, Chromium: Yttrium‐Scandium‐Gallium‐Garnet Laser: Histomorphometric and Immunohistochemical Study

“…Within the purpose of evaluating the effects of Er,Cr:YSGG laser on the bone repair process and comparing them to the effects observed with the use of conventional drills, a well-studied model of bone repair was used, and noncritical-sized defects were created in rat tibiae. 18,19,21,22 The possible variables associated with the osteotomy preparation were reduced by the standardization of procedures according to previous studies. 16,18 Parameters for laser osteotomy were selected according to the instructions of the manufacturer and as used in a previously published study.…”

“…The evaluations were performed by a single masked, calibrated examiner (JCE). The area of newly formed bone (NFB) was calculated as a percentage of the entire defect (bone defect = 100%, NFB = x %) according to previously published methodology 18 , 19 . Measurements were performed in triplicate, and the average obtained from the three analyses was considered the final value.…”

“…The area of newly formed bone (NFB) was calculated as a percentage of the entire defect (bone defect = 100%, NFB = x%) according to previously published methodology. 18,19 Measurements were performed in triplicate, and the average obtained from the three analyses was considered the final value. Quantitative data were first tested for homogeneity of variance and then tested with a one-way analysis of variance, followed by Tukey post hoc test for multiple comparisons, at 5% significance (P <0.05).…”

Bone Regeneration Using Antiosteoporotic Drugs in Adjunction with Bone Grafting: A Meta-Analysis

“…10,11 However, such alloplastic biomaterials are not capable of accommodating osteogenic cells and biological factors in situ. 10 In addition, the clinical success of bone grafting depends on several subregulatory biological mechanisms that are controlled by hormones and specific tissue factors, 12,13 which can be compromised in systemic conditions affecting bone healing. [14][15][16] For instance, in healthy adults, a nondisturbed bone-healing process will support the optimal integration of a bone substitute.…”

FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

“…Diabetes mellitus is a disorder characterized by deficient insulin production associated with metabolic changes that delay cell proliferation and reduce granulation tissue formation and collagen metabolism, consequently impairing tissue repair (Esteves et al, 2008;Galkowska et al, 2006). The number of people in the world with diabetes is increasing each year, particularly in the young population.…”