Restructuring of Vessels of the Thyroid Gland After Experimental Thermal Injury Along With Application of Minced Substrate of Freeze-Dried Xenograft

Korytskyi, V. G.; Небесна, З. М.; Kramar, S. B.

doi:10.26724/2079-8334-2019-2-68-183-187

Cited by 2 publications

(2 citation statements)

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“…1 day after the experimental thermal injury, necrectomy of the affected areas of the skin was performed and the wound was covered with a crushed substrate of lyophilized xenoskin [21,26,33]. Animal decapitation was performed on days 7, 14 and 21 of the experiment [17]. For histological examination, pieces of the adrenal glands were removed, the tissue was fixed in 10 % neutral formalin solution, dehydrated in alcohols of increasing concentration and poured into paraffin blocks.…”

Section: Methodsmentioning

confidence: 99%

Histological changes of the adrenal glands vessels after experimental thermal trauma and under the conditions of lyophilized xenoskin use

Kulbitska

2021

Biomed. and Biosoc. Anthrop.

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Burns, as a stress factor, cause circulatory and microcirculation disorders in the organs. In the scientific literature, the issues of structural reorganization of the vascular bed of the adrenal glands in thermal trauma and under conditions of correction by lyophilized xenoskin are insufficiently covered and therefore require further study. The aim of our study was to study the microscopic changes of the vessels of the adrenal glands in the dynamics after experimental thermal trauma and under conditions of correction. The experiment was performed on adult white male rats. Grade III burn (18-20 % of the skin surface) was applied under thiopental-sodium anesthesia with copper plates heated in water to a temperature of 97-1000C, on the epilated surface of the skin of the back for 10 seconds. One day after the thermal injury, an early necrectomy was performed and the wound was covered with a substrate of lyophilized xenoskin. Animal decapitation and microscopic changes were studied at days 7, 14, and 21 of injury modeling. The manufacture of histopreparations was carried out according to conventional methods. Histological examinations after experimental thermal trauma and correction of xenoskin substrate on the 7th day showed that already in this period of the experiment there were less pronounced destructive changes in the structure of the vessels of the adrenal glands. Some blood vessels were unevenly filled and dilated. The hemocapillaries of the microcirculatory tract are blood-filled, the vessel wall is destructively altered, and perivascular edema is present. On the 14th day of the experiment, moderately blood vessels were observed. The wall of large vessels is slightly swollen, partially thickened, hemocapillaries are moderately blood-filled, with dilated lumens and continuous endothelial lining. Under the conditions of application of the substrate of lyophilized xenoskin on the 21st day of the experiment, the structural organization of the vascular bed of the adrenal glands was significantly better compared to the previous terms of the study. Most vessels had evenly dilated lumens and moderate blood supply, and wall edema was insignificant. The walls of microvessels are preserved, well structured, endotheliocytes without severe damage, without signs of infiltration and edema. Thus, the results of microscopic examination showed that early necrectomy, and subsequent closure of the wound with a substrate of lyophilized xenoskin, creates optimal conditions for regenerative processes in both burns and vascular bed of the adrenal glands. On the 7th day after the burn and under conditions of correction, the study of the vascular bed of the adrenal glands showed a moderate degree of destructive changes in the vascular bed of the adrenal glands and the initial signs of their regeneration. In the late terms (14-21 days) of the experiment the relative normalization of cellular parenchymal components of the organ was established against the background of restoration of the structural organization of the microcirculatory tract.

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Section: Methodsmentioning

confidence: 99%

Histological changes of the adrenal glands vessels after experimental thermal trauma and under the conditions of lyophilized xenoskin use

Kulbitska

2021

Biomed. and Biosoc. Anthrop.

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“…It is known that thermal trauma directly causes morphofunctional changes in the skin, and indirectly affects all organs and systems [5,8,12,18]. The burn is a strong painful stimulus, which leads to a violation of neuro-humoral mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Micro- and submicroscopic changes of the cerebellar cortex 21 days after modeling the burn

2020

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The cerebellum is a complexly organized multifunctional component of the brain and a target in numerous lesions, so the study of its morphofunctional state in various pathological conditions and burns does not lose its relevance. The aim of our study was to establish the features of micro- and submicroscopic changes in the structural components of the cerebellar cortex after 21 days under conditions of experimental thermal trauma. The experimental study was simulated on white laboratory male rats. Grade III burns were applied under thiopental-sodium anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the affected area was 18-20% of the epilated surface of the body of rats. Histological changes were studied for 21 days from the beginning of the experiment. Semi-thin sections for light microscopy were stained with methylene blue, for electron microscopy the obtained ultra-thin sections were contrasted with uranyl acetate and lead citrate according to the Reynolds method. With the help of micro- and submicroscopic studies, significant alternative changes of both neurons of all layers of the cerebellar cortex and parts of the microcirculatory tract of the organ were revealed. Neurons are disorganized, their shape is changed, there is low functional activity, due to a decrease in the area of the chromatophilic substance. Often there are cell-free areas in the ganglion layer, there is a shift of Purkinje cells deep into the granular layer, and grain cells are pushed sharply into the higher molecular layer. Thus, 21 days after the experimental thermal injury, destructive-degenerative changes of neurons of the cerebellar cortex, paravasal edema and disorders of transendothelial metabolism were established.

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Restructuring of Vessels of the Thyroid Gland After Experimental Thermal Injury Along With Application of Minced Substrate of Freeze-Dried Xenograft

Cited by 2 publications

References 3 publications

Histological changes of the adrenal glands vessels after experimental thermal trauma and under the conditions of lyophilized xenoskin use

Histological changes of the adrenal glands vessels after experimental thermal trauma and under the conditions of lyophilized xenoskin use

Micro- and submicroscopic changes of the cerebellar cortex 21 days after modeling the burn

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