Space truss structures are commonly used in long-span roof structures. Recently, middle-hung scoreboards (MHS), a kind of large-scale display device flexibly suspended in the center of the roof, have been widely used in gymnasiums. However, the effect of the MHS on dynamic characteristics and earthquake response of space truss structures needs to be investigated. In this paper, the effect on the MHS under horizontal earthquake motion is studied. The simplified model, where the MHS is simplified to four fixed masses on a structure, and the flexibly suspended model are established with Abaqus software, and the earthquake response is analyzed by the time history method with the dynamic explicit method. The influence laws of the wire extent and the MHS weight are discussed. Compared with the simplified model, the nodal acceleration and the axial force rise up to 2.123 times and 1.575 times, respectively, in the flexibly suspended model, indicating that the amplification effect of the MHS acts significantly under horizontal earthquake motion. It turns out that the earthquake response of a space truss structure could be underestimated if a simplified model is used. The crest acceleration of both top chord nodes and bottom chords nodes are greatly influenced by the MHS weight but little affected by the wire extent. The influence laws of the MHS weight and the wire extent on crest axial forces of structural members are very complicated. The central regions of both the top chord and the bottom chord are the most affected regions, and the boundary regions parallel to the direction of the earthquake motion are the least affected regions. It is suggested that the envelope value under the conditions of different wire extent and MHS weight are used for structural design.