Solvent–solvent fractionations of Combretum erythrophyllum (Burch.) leave extract: Studies of their antibacterial, antifungal, antioxidant and cytotoxicity potentials

Mtunzi, Fanyana M.; Ejidike, Ikechukwu P.; Ledwaba, Imelda; Ahmed, Aroke Shahid; Pakade, Vusumzi Emmanuel; Klink, Michael J.; Modise, Sekomeng J.

doi:10.1016/j.apjtm.2017.07.007

Cited by 32 publications

(42 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ese findings correspond to a previous study of a C. erythrophyllum ethyl acetate plant extract showing a free radical scavenging activity at IC 50 4.3 μg/mL [59]. In their study Mtunzi et al [59] ranked the activity of the plant crude extracts in the following order: ethyl acetate > dichloromethane > water > acetone > hexane [59]. Interestingly, high antioxidant activity in DPPH assays is associated with phenolic compound quantity in crude extracts [80,84,85] and this can be seen in our GC/ MS findings (Table 3) where several phenolic compounds were detected and identified.…”

Section: Antimicrobial Activity and Micsupporting

confidence: 91%

“…erefore, the IC 50 of M. radiotolerans MAMP 4754 observed in this study suggests that the extract of M. radiotolerans MAMP 4754 is an effective radical scavenger and can be used as a potential antioxidant supplement. ese findings correspond to a previous study of a C. erythrophyllum ethyl acetate plant extract showing a free radical scavenging activity at IC 50 4.3 μg/mL [59]. In their study Mtunzi et al [59] ranked the activity of the plant crude extracts in the following order: ethyl acetate > dichloromethane > water > acetone > hexane [59].…”

Section: Antimicrobial Activity and Micsupporting

confidence: 88%

“…TK-VL_333 using ethyl acetate [58]. is was further confirmed in a study by Mtunzi et al [59] where ethyl acetate fractions were observed to have been the best extractant for antimicrobial compounds in an MIC assay. Taken together, the antimicrobial activity observed in the current study along with previous studies can be associated with the presence of phenolic and polyphenolic compounds [60,61].…”

Section: Antimicrobial Activity and Micmentioning

confidence: 64%

See 2 more Smart Citations

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Photolo

Mavumengwana

Sitole

et al. 2020

International Journal of Microbiology

View full text Add to dashboard Cite

is study reports on the isolation and identification of Methylobacterium radiotolerans MAMP 4754 from the seeds of the medicinal plant, Combretum erythrophyllum, for the purposes of investigating antimicrobial and antioxidant activities from this endophyte. e strain identity was confirmed by 16S rRNA-based phylogeny and Scanning Electron Microscopy (SEM). Ethyl acetate and chloroform (1 : 1 v/v) extracts from the endophyte were tested for antimicrobial and antioxidant activity on a total of 7 bacterial species (3 Gram-positive and 4 Gram-negative) using the standard Minimum Inhibitory Concentration (MIC) protocol and Quantitative Radical Scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. e MICs were recorded at 250 μg/mL for B. subtilis ATCC 19659, B. cereus ATCC 1076, E. coli ATCC1053, and 62.5 μg/mL for K. oxytoca ATCC 13182 and M. smegmatis ATCC 21293, while an IC 50 of 5.65 μg/mL was recorded with the DPPH assay. Qualitative phytochemical analysis was positive for alkaloids, flavonoids, and steroids. Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of 9-octadecene, 2,4-dinitrophenyl acetate, and 2(5H)-furanone, which have been previously reported for the targeted activities. M. radiotolerans MAMP 4754 tested positive for antimicrobial and antioxidant activity and this is linked to the production of plant-derived secondary metabolites by this strain. with only 3% of these being antimicrobial [4]. e pharmaceutical properties of medicinal plants have been linked to the production of a wide variety of structurally diverse phytochemicals which include alkaloids, flavonoids, terpenes, steroids, curcumins, saponins, and phenolics, all of which can potentially serve as drug lead candidates for the development of antimicrobials and resistance modifiers [5,6].According to the International Union for Conservation of Nature and the World Wildlife, there are an estimated 80 000 recognized medicinal flowering plant species globally,

show abstract

Section: Antimicrobial Activity and Micsupporting

confidence: 91%

Section: Antimicrobial Activity and Micsupporting

confidence: 88%

Section: Antimicrobial Activity and Micmentioning

confidence: 64%

See 1 more Smart Citation

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Photolo

Mavumengwana

Sitole

et al. 2020

International Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Screening and identification of bioactive chemical constituents in the extracts was carried out using the standard procedures with little modifications. The test carried out includes the following: alkaloids, flavonoids, phenols, carbohydrates, cardiac glycosides, saponins and proteins, steroids [16][17][18] .…”

Section: Qualitative Phytochemical Testsmentioning

confidence: 99%

Gc-MS Analysis and Extraction Optimization of Bioactive Compounds From Pelargonium Graveolens Lher Methanolic Extract and Their Activities as Pharmacological Agents

Makanyane¹,

Ejidike²,

Ssemakalu³

et al. 2019

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Poly phenols are multifunctional secondary metabolites in plants. Less information is available concerning the statistical yield, optimal extraction method and pharmacological activity with regards to active compounds from Pelargonium graveolens LʼHér leaf. Therefore, Box-Behnken design was used to optimize extraction parameters such as the speed of agitation (rpm), the volume of methanol solvent (mL), time of extraction (h) and mass of plant material (g). A polynomial quadratic model produced satisfactory fitting of experimental data: % yield (Y): R 2 = 0.9943, total phenols (TPC): R 2 = 0.9621 and total flavonoids (TFC): R 2 = 0.9873 at p < 0.05. The antioxidant activities of optimized methanol extract and controls were evaluated by DPPH, ABTS scavenging, and ferric reducing power in vitro assays. The kinetics of the lipid protective activity was studied and fitted into a pseudosecond-order kinetic model. Total antioxidant capacity (TAC) of the extract and gallic acid showed donating potential of 0.35 ± 0.10 mg QE/g and 0.35 ± 0.10 mg QE/g, respectively. The best MIC value of 1.56 mg/mL was against E. coli. The cell proliferation and cytotoxic activities of the extracts were evaluated using the Cell Titer® blue cell viability and lactate dehydrogenase assay on HaCaT cells. Bioactive compounds present in the leaf of P. graveolens were identified by GC-MS analysis by methanol extract.

show abstract

“…The 25 days grown culture was filtered and extracted with ethyl acetate and the filtrate (2 L) was dried to obtain a crude extract (620 mg). It was subjected to a modified solvent-solvent fractionation method (Mtunzi et al, 2017;Shylaja et al, 2018) for isolation of secondary metabolites. Initially, the crude extract was partitioned with petroleum ether (26 mg), chloroform (242 mg) and methanol (147 mg).…”

Section: Antimycobacterial and Anti-oxidant Potential Of The Bioactivmentioning

confidence: 99%

Antimycobacterial and anti-oxidant potential of the bioactive metabolite isolated from the endophytic fungus Daldinia eschscholtzii

Shylaja

Sasikumar

Arunachalam

2018

Bangladesh J Pharmacol

View full text Add to dashboard Cite

Solvent–solvent fractionations of Combretum erythrophyllum (Burch.) leave extract: Studies of their antibacterial, antifungal, antioxidant and cytotoxicity potentials

Abstract: It can be concluded that the extracts of C. erythrophyllum are safe for medicinal use in folk medicine for treating infectious and stress related diseases.

Cited by 32 publications

References 34 publications

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Gc-MS Analysis and Extraction Optimization of Bioactive Compounds From Pelargonium Graveolens Lher Methanolic Extract and Their Activities as Pharmacological Agents

Antimycobacterial and anti-oxidant potential of the bioactive metabolite isolated from the endophytic fungus Daldinia eschscholtzii

Contact Info

Product

Resources

About