Spectroscopic determination of succinylcholine in dosage forms using eosin Y

Abstract

Two simple and sensitive analytical assay methods using spectrophotometry and spectrofluorimetry techniques were developed for the estimation of succinylcholine chloride (SUC) in pharmaceutical preparations. The suggested methods are based on the formation of an ion pair complex formed between the drug and eosin Y spectrophotometrically (Method I), or the suppressive effect of succinylcholine on the native fluorescence property of eosin Y (Method II). The spectrophotometric method (Method I) involves measuring the absorbance of the complex between succinylcholine and eosin Y at 550 nm in Britton Robinson buffer of pH 3. However, the spectrofluorimetric method (Method II) involves measuring the quenching effect of the studied drug on the native fluorescence property of eosin Y at the same pH at 550 nm after excitation at 480 nm. The absorbance versus concentration of the drug is rectilinear over the range of 0.5 to 15 μg/ml. The formation constant was 3.5 × 10 and the Gibb's free energy change was -2.5 × 10 J/mol. In Method II, the relative fluorescence intensity was directly proportional to SUC concentration over the range of 0.05 to 1 μg/ml. The proposed methods allowed a successful application to the estimation of succinylcholine ampoules. An explanation of the reaction pathway was postulated.

Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride

“…EY is a carboxylic dye that is used as an ion-pairing reagent for the spectroscopic determination of cationic drugs in an acidic medium by forming ion-pair complexes [11][12][13][14][15]. In the spectrophotometric method, the ABS of the developed complex was measured at 550 nm (figure 2).…”

“…Additionally, the experimental conditions were optimized using the design of experiments (DOEs). Some amino compounds can be determined using an acidic dye called EY as an ion-pairing agent (figure 1b) via binary complex formation [11][12][13][14][15]. DOE is a powerful data collection and analysis tool that can be used under various experimental conditions [16].…”

Application of a xanthene dye, eosin Y, as spectroscopic probe in chemical and pharmaceutical analysis; a review

“…The solution was slightly acidic (pH 2.2-4.0) and the absorbance was measured at wavelengths in the range of 540-552 nm. The majority of the formed complexes were freely soluble in water and therefore, the extraction with an organic solvent or the addition of a surfactant was not required (Kovacs-Hadady & Fabian, 1998;El-Brashy, El-Sayed Metwally & El-Sepai, 2004;Walash et al 2007;2010;Li et al, 2011;Walash et al, 2011;Ammar, El-Brashy & Al-Sahly, 2014;Hussein, Fares & El-Kosasy, 2014;Ayad et al, 2018;Naggar et al, 2017;El-Masry et al, 2018). However, in a few cases (Gazy et al, 2002;Derayea et al, 2012;Derayea, 2014;El-Hay, El-Mammli & Shalaby, 2016), it was necessary to enhance the water solubility of the complex through the addition of non ionic surfactant such as methyl cellulose.…”

“…In most cases, the composition of the formed binary complex was in the ratio of 1:1, eosin:compounds. However, in some rare cases, a complex containing 2:1, drug:eosin, was formed due to the presence of two active basic centers in the drug such as azithromycin (Walash et al 2007) and succinylcholine (Ayad et al 2018). In the case of ternary complexes, the target analytes form chelates with metal ions followed by further complexation of the formed chelates with eosin anion.…”