Stability and conformational change of methoxypolyethylene glycol modification for native and unfolded trypsin

Liu, Wei; Liu, Junping; Zou, Liqiang; Zhang, Zhao-Qin; Liu, Chengmei; Liang, Ruihong; Xie, Ming‐Jin; Wan, Jie

doi:10.1016/j.foodchem.2013.09.067

Cited by 29 publications

(8 citation statements)

References 26 publications

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“…12 As shown, the conformational change reflected in the reduction of fluorescence intensity could result from the change of interactions between spermidine and all residues of trypsin, which contributed to the increase in surface hydrophilicity. 12 3.3. Mode of Fluorescence Quenching.…”

Section: Resultsmentioning

confidence: 91%

“…11 Trypsin breaks the peptide bonds at the C-terminal ends of lysine, arginine, and ornithine residues, which is widely applicable in protein analysis, biotechnology, food industry, and in biomedicine. 12 The stability of the enzymatic activity of trypsin has been studied in different solvent media 11,12 biological processes. Literature survey shows that there has been no work on the interaction of spermidine with trypsin.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Studies on the Interaction of Spermidine with Bovine Trypsin by Multispectroscopic and Docking Methods

Momeni

Shareghi

Saboury³

et al. 2016

J. Phys. Chem. B

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The effect of spermidine on the kinetics, conformation, and dynamics of native trypsin was studied by steady-state thermal stability, intrinsic fluorescence, circular dichroism (CD), ultraviolet-visible (UV-vis) spectroscopy, and kinetic techniques, as well as molecular docking, at the temperatures of 298 and 308 K. The Stern-Volmer quenching constants (Ksv) for the trypsin-spermidine complex were obtained at two temperatures, revealing that spermidine quenched the intensity of trypsin through the static mode of the quenching mechanism. The corresponding thermodynamic parameters, Gibbs free-energy, enthalpy, and entropy changes, showed that the binding process was spontaneous. These values and the molecular docking technique revealed that the hydrogen bonding and van der Waals forces played a major role in stabilizing the complex. CD, absorption, and fluorescence results also indicated that spermidine binding had a partial effect on trypsin structure. Spermidine could also influence the activity of trypsin. Upon spermidine binding, the Vmax value of the enzyme was increased and the kcat/Km values were enhanced slightly. The Tm of the trypsin-spermidine complex was enhanced probably due to the higher H-bond formation and lower surface hydrophobicity after spermidine modification, as confirmed by UV-vis spectroscopy and fluorescence spectra. UV absorption and CD studies also indicated that the binding of spermidine to trypsin had induced microenvironmental changes around the enzyme, leading to changes in its secondary structure.

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Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Comparative Studies on the Interaction of Spermidine with Bovine Trypsin by Multispectroscopic and Docking Methods

Momeni

Shareghi

Saboury³

et al. 2016

J. Phys. Chem. B

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show abstract

“…This device uses a high-pressure positive displacement pump (the pressure range is approximately 5-200 MPa). This equipment has been traditionally used in the pharmaceutical industry to make pharmaceutical emulsions as well as in the food industry to produce nanosystems 10,11 or homogenized proteins (milk, whey protein, trypsin, and so on) [12][13][14][15] and dietary fiber 16 only in the last few years. High-pressure microfluidization uses the combined forces of high-velocity impact, high-frequency vibration, instantaneous pressure drop, intense shear, cavitation, and ultra-high pressures up to 200 MPa with a short treatment time (less than 5 seconds) and continuous operation.…”

Section: High-pressure Microfluidizationmentioning

confidence: 99%

A Modern Technology Applied in Traditional Chinese Medicine: Progress and Future of the Nanotechnology in TCM

et al. 2019

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The application of nanotechnology to traditional Chinese medicine (TCM) enabled the development of Chinese medicine in the international society. The pharmacodynamics of TCM is not only depending on its chemical constituents but also related to its physical state such as particle size. Indeed, there is some new pesticide effect that appeared when the medicine was being made into nanophase. The application of nanotechnology to TCM can expand the use of a range of Chinese medicinal materials. In this review, we introduce the concept of nanometer TCM. We also review the preparation methods, advantages, and development tendency of Nano-TCM; furthermore, we analyze the problems in the process of development of Nano-TCM and put forward varies possible solutions to solve this problems, thereby providing new thought for the development of Nano-TCM.

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“…Trypsin, excreted by the pancreas into the small intestine, participates in the digestion of food proteins and other biological processes in human body, and is usually used as an important model of the digestive proteases to investigate the interactions between drugs and proteins. Previous studies have shown that drugs can interact with trypsin and then affect the structure and properties of trypsin [7]. For example, bifendate or analogs (I, II and III) interacted with trypsin to form a complex which led to the conformational and functional changes of trypsin [8].…”

Section: Introductionmentioning

confidence: 99%

Binding characteristics of psoralen with trypsin: Insights from spectroscopic and molecular modeling studies

Liu

Zhang

Liao

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Stability and conformational change of methoxypolyethylene glycol modification for native and unfolded trypsin

Cited by 29 publications

References 26 publications

Comparative Studies on the Interaction of Spermidine with Bovine Trypsin by Multispectroscopic and Docking Methods

Comparative Studies on the Interaction of Spermidine with Bovine Trypsin by Multispectroscopic and Docking Methods

A Modern Technology Applied in Traditional Chinese Medicine: Progress and Future of the Nanotechnology in TCM

Binding characteristics of psoralen with trypsin: Insights from spectroscopic and molecular modeling studies

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