Stimulation of wound healing, using brain extract with fibroblast growth factor (PGF) activity

Buntrock, P; Jentzsch, K.D.; Heder, G.

doi:10.1016/s0232-1513(82)80054-6

Cited by 50 publications

(6 citation statements)

References 9 publications

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“…Stimulation of epithelial cell proliferations and angiogenesis are vital for wound healing process to take place [42]. Wound epithelialization is a process whereby there is epithelial regeneration post wounding with the epithelial cells proliferating and migrating over the wound bed, thereby providing a protective cover for the freshly formed tissues [43].…”

Section: Discussionmentioning

confidence: 99%

Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats

Udegbunam

Muogbo

et al. 2014

BMC Complement Altern Med

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BackgroundWound healing is a natural process that enables tissue repair after an injury. To shorten its duration and minimize associated complications, wounds are treated with medications. Currently there is a growing interest in the use of alternative wound dressing agents such as plant extracts. One plant used traditionally in wound treatment is Pupalia lappacea. In view of its use in wound care, we investigated the wound healing activities of 80% methanolic leave extract of Pupalia lappacea using excision, incision and dead space wound models. Also its effects on three common wound contaminants were investigated.MethodsExcision wounds were created, contaminated with microbes and treated with ointments (10% and 20% w/w) prepared from Pupalia lappacea. Incision and dead space wounds were also created in rats which were subsequently dosed orally with the extract. The wound healing activities of Pupalia lappacea ointment on excision wound was assessed by rates of wound contraction and epithelialization as well as its antibacterial effects. The effects of Pupalia lappacea on incision and dead-space wounds were determined by the wound breaking strengths and weights of the granuloma tissues formed, respectively.ResultsPupalia. lappacea ointments significantly (p < 0.05) accelerated wound healing with 20% ointment having the highest percentage wound contraction and rate of epithelialization. At 4, 7 and 14 days post treatment, mean total viable bacterial count of excision wounds of the extract treated groups were significantly (p < 0.05) lower compared against the control. Wound breaking strengths and weights of granuloma tissues formed in the extract treated groups were significantly (p < 0.05) higher than those of the control group. The minimum inhibitory concentration values obtained for the Pupalia lappacea extract against Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis were 9 mg/ml, 4 mg/ml and 3 mg/ml, respectively, while the corresponding minimum bactericidal concentrations were 10 mg/ml, 8 mg/ml and 7 mg/ml.ConclusionThe results obtained showed that Pupalia. lappacea has good wound healing and antibacterial activities. These findings validate the use of this plant in traditional medicine for treatment of wounds.

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Section: Discussionmentioning

confidence: 99%

Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats

Udegbunam

Muogbo

et al. 2014

BMC Complement Altern Med

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“…hFGF-2 is a potent mitogen for endothelial cells [3] and for other cells of mesodermal and neuroectodermal origin [1,2]. The wound-healing activity of hFGF-2 [4,5] renders it a potential therapeutic agent of industrial importance [6,7].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility towards intramolecular disulphide-bond formation affects conformational stability and folding of human basic fibroblast growth factor

Estapé¹,

Heuvel²,

Rinas³

1998

Biochemical Journal

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The conformational stability and the folding properties of the all-beta-type protein human basic fibroblast growth factor (hFGF-2) were studied by means of fluorescence spectroscopy. The results show that the instability of the biological activity of hFGF-2 is also reflected in a low conformational stability of the molecule. The reversibility of the unfolding and refolding process was established under reducing conditions. Determination of the free-energy of unfolding in the presence of reducing agents revealed that the conformational stability of hFGF-2 (DeltaGH2Oapp congruent with21 kJ. mol-1, 25 degreesC) is low compared with other globular proteins under physiological conditions (20-60 kJ.mol-1). However, the conformational stability of hFGF-2 is particularly low under non-reducing conditions. This instability is attributed to intramolecular disulphide-bond formation, rendering the molecule more susceptible to denaturant-induced unfolding. In addition, denaturant-induced unfolding of hFGF-2 renders the protein more susceptible to irreversible oxidative denaturation. Experimental evidence is provided that the irreversibility of the unfolding and refolding process in the absence of reducing agents is linked to the formation of an intramolecular disulphide bond involving cysteines 96 and 101.

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“…For example, acidic and basic FGFs are mitogenic for a number of cell types including those of mesenchymal, epithelial, or neural origin (12,13). Consequently, studies directed toward in vivo wound healing and nerve regeneration have been initiated (14,15).…”

mentioning

confidence: 99%

Ligand-affinity cloning and structure of a cell surface heparan sulfate proteoglycan that binds basic fibroblast growth factor.

Kiefer¹,

Stephans²,

Crawford³

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

231

116

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Expression cloning of cDNAs encoding a basic fibroblast growth factor (FGF) binding protein confirms previous hypotheses that this molecule is a cell-surface heparan sulfate proteoglycan. A cDNA library constructed from a hamster kidney cell line rich in FGF receptor activity was transfected into a human lymphoblastoid cell line. Clones expressing functional basic FGF binding proteins at their surfaces were enriched by panning on plastic dishes coated with human basic FGF. The amino acid sequence deduced from the isolated cDNAs revealed several interesting features, including hydrophobic signal and transmembrane domains that flank an extracellular region containing six potential attachment sites for glycosaminoglycan side chains. The structure also contains a short hydrophilic cytoplasmic tail sequence homologous to previously reported actin binding domains. Binding of basic FGF to cells expressing the binding protein could be inhibited by heparin and heparan sulfate but not by chondroitin sulfate, dermatan sulfate, or keratan sulfate. In addition to binding basic FGF, this protein or related surface proteins may function as an initial cellular attachment site for other growth factors and for viruses, such as herpes simplex virus.

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Stimulation of wound healing, using brain extract with fibroblast growth factor (PGF) activity

Cited by 50 publications

References 9 publications

Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats

Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats

Susceptibility towards intramolecular disulphide-bond formation affects conformational stability and folding of human basic fibroblast growth factor

Ligand-affinity cloning and structure of a cell surface heparan sulfate proteoglycan that binds basic fibroblast growth factor.

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