The effect of dietary amylose/amylopectin ratio on serum and hepatic lipid content and its molecular mechanisms in growing‐finishing pigs

Yang, Can; He, Jun; Yu, Bing; Yu, Jie; Mao, Xiangbing; Chen, D. W.; Yin, Yulong

doi:10.1111/jpn.12884

Cited by 12 publications

(12 citation statements)

References 31 publications

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“…We found that the mRNA level of FAS in piglets of the low AR (AR 0.31 and 0.14) groups were higher than those of the high AR groups. The present results, however, agree well with previous findings showing that ingestion of a low AR diet significantly elevated the lipogenic gene expression in growing-finishing pigs [31] and other animals [32,33].…”

Section: Discussionsupporting

confidence: 93%

Effects of Dietary Starch Structure on Growth Performance, Serum Glucose–Insulin Response, and Intestinal Health in Weaned Piglets

Gao

et al. 2020

Animals

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To investigate the effects of dietary starch structure (amylose/amylopectin ratio, AR) on serum glucose absorption metabolism and intestinal health, a total of ninety weaned piglets (Duroc × (Yorkshire × Landrace)) were randomly assigned to 5 dietary treatments and fed with a diet containing different AR (2.90, 1.46, 0.68, 0.31, and 0.14). The trial lasted for 21 d. In this study, the growth performance was not affected by the dietary starch structure (p > 0.05). Diets with higher amylose ratios (i.e., AR 2.90 and 1.46) led to a significant reduction of the serum glucose concentration at 3 h post-prandium (p < 0.01), while high amylopectin diets (AR 0.31 and 0.14) significantly elevated The expression of gene s at this time point (p < 0.01). High amylopectin diets also increased the apparent digestibility of crude protein (CP), ether extract (EE), dry matter (DM), gross energy (GE), and crude ash (p < 0.001). Interestingly, diet rich in amylose (AR 2.90) significantly elevated the butyric acid content (p < 0.05) and decreased the pH value (p < 0.05) in the cecal digesta. In contrast, diet rich in amylopectin (i.e., AR 0.14) significantly elevated the total bacteria populations in the cecal digesta (p < 0.001). Moreover, a high amylopectin diet (AR 0.14) tended to elevate the mRNA level of fatty acid synthase (FAS, p = 0.083), but significantly decreased the mRNA level of sodium-dependent glucose transporter 1 (SGLT1, p < 0.05) in the duodenal and jejunal mucosa, respectively. These results suggested that blood glucose and insulin concentrations were improved in high AR diets, and the diet also helped to maintain the intestinal health.

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Section: Discussionsupporting

confidence: 93%

Effects of Dietary Starch Structure on Growth Performance, Serum Glucose–Insulin Response, and Intestinal Health in Weaned Piglets

Gao

et al. 2020

Animals

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“…Despite the slight increase (+ 5%) of the hepatic PC:PE-ratio in pigs of group IM10 was statistically significant, we postulate that this increase is not of biological relevance considering the marked elevation (+ 35%) of the hepatic PC:PE-ratio in severely steatotic obese Zucker rats compared with non-steatotic lean Zucker rats [16]. In line with our assumption, there was no indication of fatty liver induction as evident from the observation that hepatic TG concentrations of the pigs of all groups were within the physiological range reported from others [58,59]. With regard to individual phospholipid species, we observed that the percentages of PC and PE species with 6 double bonds were reduced in the liver of pigs of group IM10.…”

Section: Discussionsupporting

confidence: 82%

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in growing pigs by transcriptomics, metabolomics and lipidomics

Meyer

Geßner

Braune

et al. 2020

J Animal Sci Biotechnol

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Background: The hypothesis was tested that insect meal (IM) as protein source influences intermediary metabolism of growing pigs. To test this, 30 male, 5-week-old crossbred pigs were randomly assigned to 3 groups of 10 pigs each with similar body weights (BW) and fed isonitrogenous diets either without (CON) or with 5% IM (IM5) or 10% IM (IM10) from Tenebrio molitor L. for 4 weeks and key metabolic tissues (liver, muscle, plasma) were analyzed using omics-techniques. Results: Most performance parameters did not differ across the groups, whereas ileal digestibilities of most amino acids were 6.7 to 15.6%-units lower in IM10 than in CON (P < 0.05). Transcriptomics of liver and skeletal muscle revealed a total of 166 and 198, respectively, transcripts differentially expressed between IM10 and CON (P < 0.05). Plasma metabolomics revealed higher concentrations of alanine, citrulline, glutamate, proline, serine, tyrosine and valine and a lower concentration of asparagine in IM10 than in CON (P < 0.05). Only one out of fourteen quantifiable amino acid metabolites, namely methionine sulfoxide (MetS), in plasma was elevated by 45% and 71% in IM5 and IM10, respectively, compared to CON (P < 0.05). Plasma concentrations of both, major carnitine/ acylcarnitine species and bile acids were not different across groups. Lipidomics of liver and plasma demonstrated no differences in the concentrations of triacylglycerols, cholesterol and the main phospholipids, lysophospholipids and sphingolipids between groups. The percentages of all individual phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species in the liver showed no differences between groups, except those with 6 double bonds (PC 38:6, PC 40:6, PE 38:6, PE 40:6), which were markedly lower in IM10 than in CON (P < 0.05). In line with this, the percentage of C22:6n-3 in hepatic total lipids was lower in IM10 than in the other groups (P < 0.05). Conclusions: Comprehensive analyzes of the transcriptome, lipidome and metabolome of key metabolic tissues indicate that partial or complete replacement of a conventional protein source by IM in the diet has only a weak impact on the intermediary metabolism of growing pigs. Thus, it is concluded that IM from Tenebrio molitor L. can be used as a dietary source of protein in pigs without causing adverse effects on metabolism.

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“…Previous studies indicated that dietary change widely affected endogenous enzymes activities (Yang, He, Yu, Yu, & Yin, 2018), while the current study showed that dietary different AM/AP ratio failed to influence α‐amylase, chymotrypsin, trypsin, lipase, sucrase and maltase activities. The reason might be caused by the weak secretory ability of young animals that broilers aged from 1 to 21 days were selected in this study.…”

Section: Discussioncontrasting

confidence: 46%

Effects of dietary amylose/amylopectin ratio and amylase on growth performance, energy and starch digestibility, and digestive enzymes in broilers

Yang

et al. 2020

Animal Physiology Nutrition

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Aims and scopeThe Journal of Animal Physiology and Animal Nutrition publishes original papers on hypothesis-driven research in the fields of animal physiology, physiology and biochemistry of nutrition, animal nutrition, feed technology, and feed preservation but not manuscripts presenting fish (or other aquatic non-mammals) nutritional or growth topics. The journal is a forum for presenting articles on basic and applied research, thus making new findings, methods, and techniques easily accessible and applicable in practice. Reviews of the most important specialized literature are also included.

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The effect of dietary amylose/amylopectin ratio on serum and hepatic lipid content and its molecular mechanisms in growing‐finishing pigs

Cited by 12 publications

References 31 publications

Effects of Dietary Starch Structure on Growth Performance, Serum Glucose–Insulin Response, and Intestinal Health in Weaned Piglets

Effects of Dietary Starch Structure on Growth Performance, Serum Glucose–Insulin Response, and Intestinal Health in Weaned Piglets

Comprehensive evaluation of the metabolic effects of insect meal from Tenebrio molitor L. in growing pigs by transcriptomics, metabolomics and lipidomics

Effects of dietary amylose/amylopectin ratio and amylase on growth performance, energy and starch digestibility, and digestive enzymes in broilers

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