The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats

Abstract

Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3 rd , 7 th and 12 th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3 rd , 7 th , 12 th day, the results were p<0.05. In the treatment group on the 3 rd day compared to the 7 th and 12 th days the results were p>0.05. The treatment group on the 7 th day compared to the treatment group on the 12 th day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3 rd day was compared with the 7 th day. The 12 th also had a result of p<0.05. The comparison between the 7 th and 12 th day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.

The Effectiveness of The Combination of Nanoparticles from Carica papaya L. Leaf and Chitosan 1% Against Fibroblasts in The Tooth Socket of Wistar Rats.

“…Flavonoids have antiinflammatory and analgesic activity, which affects the increase in the number of fibroblasts in the proliferation phase. 7 The results of the particle size analyzer test in this study showed that the average nanoparticle size was smaller than the results of other studies, where B. Nugroho et al, (2017) obtained a size of 77.1 nm. The smaller the particle size of an active substance in the nanoemulsion preparation will further increase its stability and spread in the dissolution medium so that it is more effective in increasing the number of fibroblast cells in the tooth extraction socket.…”

“…[4][5][6] One of the natural ingredients used to accelerate the wound healing process is the leaves of Carica papaya L. which contain flavonoids, tannins, saponins, papain and alkaloids, because the content contained in the leaves of Carica papaya L. causes it to have medicinal properties. [7][8][9][10] Nowadays, nanotechnology is widely used in the health sector, one of which is the administration of drugs with nanoparticles which can make drugs easier to enter and can provide a quick reaction to the body.…”

Effect of snail mucus on angiogenesis during wound healing

“…The hypoxic state of the tissue becomes an angiogenic stimulator as growth factors and cytokines are released from inflammatory cells accumulated in the wound area during the previous inflammatory process. These factors stimulate the proliferation and invasiveness of vascular cells to promote blood vessel growth 15,16,40 .…”

Effect of Papaya Leaf Extract Gel (Carica papaya) on Incision Wound Healing in Rattus norvegicus

“…Sift the liquid extract using a filter cloth, accommodating the filtrate in an Erlenmeyer. The macerated obtained are then filtered and evaporated using a rotary evaporator, and the extract is concentrated with a water bath to obtain a thick extract (Femilian et al, 2019).…”

“…In contrast, the negative control group (NaCl solution) does not contain efficacious substances for wound healing. However, the wound closes independently because the body can protect and restore itself naturally (Femilian et al, 2019). The sooner the wound is closed, the better it reduces the risk of infection and discomfort due to the wound.…”