The effects of topical application of sunflower-seed oil on open wound healing in lambs

Abstract

PURPOSE: To demonstrate the effects of the use of sunflower seed oil on the treatment of skin wounds. METHODS: Eighteen male Saint Inês lambs were divided in 3 groups according to the pos-operative (7, 14 and 21 days). After antisepsis and local anestesia, two 4cm² wounds on each side of the thoracic region, close to the scapule were surgically produced. The experimental wounds were treated with sunflower seed oil, with high concentration of linoleic acid (LA), and the control ones with sterilized Vaseline. Biopsies of the pos-operative wounds tissue were performed on the 7th, 14th, 21st days and histologically evaluated. RESULTS: Topic application of sunflower seed oil accelerated healing process at the 7th and 21st days, reducing wound area and increasing wound contraction. Granulation tissue increased faster on treated wounds. The epidermis of the treated wounds was completely recovered when compared to control wounds. CONCLUSION: The topic use of sunflower seed oil accelerated the healing process, and it can be used as an alternative therapy on second intention wound healing.

Effect of a combination of medium chain triglycerides, linoleic acid, soy lecithin and vitamins A and E on wound healing in rats

“…In the Control group, gaps in the scab were seen on the 7 th day, along with irregular edges due to contraction of the wound, besides the presence of granulation tissue. The Reference group remained with the largest wound area and with irregular edges, which was also found in a study using linoleic acid 15 . The treatments in the three groups contributed to the almost complete closing of the lesions, suggesting that growth factors were probably responsible for the hyperplasia of the epithelium as reported in the literature 17 .…”

“…On the 12 th day, clinical observation showed a smaller wound area characterized by the proximity of the edges (contraction) with irregular outlines and better presence of granulation tissue, like that seen in the study with linoleic acid in sheep 15 .…”

“…The degree of wound repair (DR) of the ulcers was determined on days 3, 7 and 12, where it was calculated based on the initial area (measured on day zero) and expressed as a percentage, according to the following formula 15,16 .…”

“…In this manner, besides the objective quantification, it was also possible to perform a subjective macroscopic clinical evaluation similar to that described in other studies carried out with the same test substance in dogs 9 and with linoleic acid in sheep 15 .…”

“…A study that examined wound healing in sheep showed that linoleic acid constituted a powerful pro-inflammatory mediator, being essential for the regulation of the biochemical events that precede fibroplasia in addition to stimulating growth factors and neovascularization 15 . It is possible that in this study the presence of linoleic acid in the test preparation contributed to a similar event.…”