The heat shock protein ClpB mediates the development of thermotolerance in the cyanobacterium Synechococcus sp. strain PCC 7942

Eriksson, Mats-Jerry; Clarke, Adrian K.

doi:10.1128/jb.178.16.4839-4846.1996

Cited by 98 publications

(104 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inactivation of several other genes, such as clpB (protease), hspA, htpG, dnaK2 (chaperones), sigB and sigC (sigma factors), induces thermal sensitivity [21][22][23][24]. In addition, the Hik34 histidine kinase is believed to down regulate the expression of several heat shock genes as the Synechocystis Dhik34 deletion mutant shows increased thermal tolerance [25].…”

Section: Proteomics Of the Heat Shock Responsementioning

confidence: 99%

Proteomic analyses of the response of cyanobacteria to different stress conditions

Castielli¹,

Cerda²,

Navarro³

et al. 2009

FEBS Letters

View full text Add to dashboard Cite

a b s t r a c tCyanobacteria are significant contributors to global photosynthetic productivity, thus making it relevant to study how the different environmental stresses can alter their physiological activities. Here, we review the current research work on the response of cyanobacteria to different kinds of stress, mainly focusing on their response to metal stress as studied by using the modern proteomic tools. We also report a proteomic analysis of plastocyanin and cytochrome c 6 deletion mutants of the cyanobacterium Synechocystis sp. PCC 6803 grown under copper or iron deprivation, as compared to wild-type cells, so as to get a further understanding of the metal homeostasis in cyanobacteria and their response to changing environmental conditions.

show abstract

Section: Proteomics Of the Heat Shock Responsementioning

confidence: 99%

Proteomic analyses of the response of cyanobacteria to different stress conditions

Castielli¹,

Cerda²,

Navarro³

et al. 2009

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Synthesis of HSPs occurs at different temperatures in different organisms (Parsell and Lindquist 1993). A number of HSPs have been identified in various species of coral (Black et al 1995;Hayes and King 1995;Fang et al 1997;Sharp et al 1997), symbiotic dinoflagellates (Downs et al 2000), and cyanobacteria (Borbely and Suranyi 1988;Eriksson and Clarke 1996;Roy et al 1999).…”

Section: Interpretation Of Numerical Fluorescence Values-cautionmentioning

confidence: 99%

Changes in the fluorescence of the Caribbean coral Montastraea faveolata during heat‐induced bleaching

Zawada

Jaffe

2003

Limnology & Oceanography

View full text Add to dashboard Cite

In order to evaluate the response of commonly occurring green and orange fluorescent host-based pigments, a thermal stress experiment was performed on specimens of the Caribbean coral Montastraea faveolata. Seven paired samples were collected from a small oceanic reef near Lee Stocking Island in the Bahamas. Seven of the fourteen corals were subjected to elevated temperatures for 28 d, followed by a recovery period lasting 53 d. Throughout the experiment, high-resolution (ϳ400 m pixel Ϫ1 ) multispectral images of induced fluorescence were recorded at wavelengths corresponding to the green and orange host pigments, plus chlorophyll. These images revealed that the fluorescence of both host pigments was concentrated at polyp centers and declined by 70-90% in regions between polyps. Chlorophyll fluorescence, however, was distributed almost uniformly across the entire coral surface, but with decreases of 10-30% around polyp centers. A normalized difference ratio between the green and orange pigments (GO ratio) was developed to facilitate comparison with chlorophyll fluorescence as a bleaching indicator. Analysis showed a high correspondence between a sustained GO ratio of less than zero and the death of corals. Finally, this ratio was resistant to contamination from other sources of chlorophyll fluorescence, such as filamentous algae.

show abstract

“…The gene encoding ClpB1 (Slr1641) was upregulated, this molecular chaperone has a role in acquisition of thermotolerance in the cyanobacterium Synechococcus sp. strain PCC 7942 (Eriksson & Clarke, 1996).…”

Section: The Absence Of Hik31 Alters Transcriptional Response To Low mentioning

confidence: 99%

Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and -independent responses

2011

View full text Add to dashboard Cite

We have investigated the response of the cyanobacterium Synechocystis sp. PCC 6803 during growth at very low O 2 concentration (bubbled with 99.9 % N 2 /0.1 % CO 2 ). Significant transcriptional changes upon low-O 2 incubation included upregulation of a cluster of genes that contained psbA1 and an operon that includes a gene encoding the two-component regulatory histidine kinase, Hik31. This regulatory cluster is of particular interest, since there are virtually identical copies on both the chromosome and plasmid pSYSX. We used a knockout mutant lacking the chromosomal copy of hik31 and studied differential transcription during the aerobiclow-O 2 transition in this DHik31 strain and the wild-type. We observed two distinct responses to this transition, one Hik31 dependent, the other Hik31 independent. The Hik31-independent responses included the psbA1 induction and genes involved in chlorophyll biosynthesis. In addition, there were changes in a number of genes that may be involved in assembling or stabilizing photosystem (PS)II, and the hox operon and the LexA-like protein (Sll1626) were upregulated during low-O 2 growth. This family of responses mostly focused on PSII and overall redox control. There was also a large set of genes that responded differently in the absence of the chromosomal Hik31. In the vast majority of these cases, Hik31 functioned as a repressor and transcription was enhanced when Hik31 was deleted. Genes in this category encoded both core and peripheral proteins for PSI and PSII, the main phycobilisome proteins, chaperones, the ATP synthase cluster and virtually all of the ribosomal proteins. These findings, coupled with the fact that DHik31 grew better than the wild-type under low-O 2 conditions, suggested that Hik31 helps to regulate growth and overall cellular homeostasis. We detected changes in the transcription of other regulatory genes that may compensate for the loss of Hik31. We conclude that Hik31 regulates an important series of genes that relate to energy production and growth and that help to determine how Synechocystis responds to changes in O 2 conditions.

show abstract

The heat shock protein ClpB mediates the development of thermotolerance in the cyanobacterium Synechococcus sp. strain PCC 7942

Cited by 98 publications

References 51 publications

Proteomic analyses of the response of cyanobacteria to different stress conditions

Proteomic analyses of the response of cyanobacteria to different stress conditions

Changes in the fluorescence of the Caribbean coral Montastraea faveolata during heat‐induced bleaching

Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and -independent responses

Contact Info

Product

Resources

About