The Importance of FACS Analysis in the Development of Aptamers Specific to Pathogens

Abstract

Purpose: This review aims to introduce aptamers and the methods of its development to improve the sensitivity and selectivity to target bacteria. In this review, we have highlighted current developments and directions in the pathogen detection based on aptamers. Background: Aptamers, the specific nucleic acid sequences, can bind to targets with high affinity and specificity. Some of researches on the use of aptamers for the detection of pathogen have been reported in recent years. Aptamers have more applicability than antibodies for the development of pathogen detection using biosensor; such as easy to synthesis and labeling, lack of immunogenicity, and a low cost of production. However, only few reports on the development and use of aptamers for the detection of pathogen have been published. Review: Aptamers specific to pathogen are obtained by whole-cell systematic evolution of ligands by exponential enrichment (SELEX) process. SELEX process is composed of screening random oligonucleotide bound with target cells, multiple separation and amplification of nucleic acids, final identification of the best sequences. For improving those affinity and selectivity to target bacteria, optimization of multiple separating process to remove unbounded oligonucleotides from aptamer candidates and sorting process by flow cytometry are required.

Development of ssDNA aptamers for the capture and detection of Salmonella typhimurium

“…[5][6][7] Various SELEX methods have been developed in order to suit specic requirements. 8 Cell-SELEX was developed to recognize and bind to the molecules on the surface of live cells: even though the normal sequence cannot bind to a structure, the oligonucleotide aptamer can have a specic 3D structure and strongly recognize a binding site on the cell surface. 2 Therefore, cell-SELEX methodology has recently been applied in biosensor systems, instead of antibodies.…”

A Review on Lateral Flow Test Strip for Food Safety

“…Aptamers are stable over various conditions of pH and temperature. In addition, they can be easily modified to have active groups or probes (Moon et al, 2014;Chen and Yang, 2015). Bruno (2014) developed the sandwich format LFTSs for E. coli, L. monocytogenes, and Salmonella enterica by the use of DNA aptamers.…”