The nerve protection and in vivo therapeutic effect of <em>Acalypha indica</em> extract in frogs

Abstract

Tujuan Membuktikan efek neuroproteksi dan/atau neuroterapi ekstrak air Acalypha indica Linn. pada katak yang dilumpuhkan dengan injeksi pankuronium bromida Metode Enam puluh ekor katak (Bufo melanostictus Schneider) dibagi ke dalam dua kelompok perlakuan yaitu kelompok neuroproteksi dan neuroterapi. Setiap kelompok dibagi ke dalam 6 subkelompok yaitu kontrol negatif (diberi air); kontrol positif (pirasetam), dan 4 kelompok dosis ekstrak: 200, 300, 400, 500 mg/KgBB. Pankuronim bromida 0,2 %, pelumpuh otot, disuntikkan subkutan pada punggung katak. Untuk efek proteksi, ekstrak diberikan per oral 1 jam sebelum disuntik, sedangkan untuk efek terapi ekstrak diberikan, sepuluh menit sesudah penyuntikan pankuronium bromida. Parameter yang diukur adalah waktu (menit) timbul dan lama kelumpuhan, serta waktu untuk perbaikan. Hasil penelitian menunjukkan bahwa pada dosis ekstrak 400 dan 500 mg/KgBB terjadi efek neuroproteksi yang berbeda bermakna dibanding kontrol negatif dan pirasetam (p<0.05). Ekstrak pada dosis 200-500 mg/KgBB menunjukkan efek neuroterapi yang berbeda bermakna dibandingkan kontrol negatif (p=0,000) dan tidak berbeda bermakna dibandingkan pirasetam, kecuali pada dosis ekstrak 300 mg/KgBB, menunjukkan efek lebih baik dibandingkan dengan pirasetam (p=0,012). Kesimpulan Bahwa ekstrak air Acalypha indica Linn. memiliki efek neuroproteksi dan neuroterapi yang sama atau lebih baik dibandingkan pirasetam pada katak yang dilumpuhkan dengan pankuronium bromida. Namun, masih diperlukan uji farmakodinamik dan farmakokinetik pada dua model hewan coba yang lazim digunakan.

Increased cell viability and proliferation in post-hypoxic hippocampal tissue culture treated with<em> Acalypha indica</em> root extract

“…The result of this examination showed that Acalypha indica Linn root extract has an effect on increasing the number of viable post-hypoxic nerve cells. The result was consistent with research by Purwaningsih et al 14 in which the optimal neurotherapy dose was seen at dose 20 mg/mL. The dose-dependent increase in relative cell viability raises the possibility that a higher dose than 20 mg/mL would further enhance the cell viability, thus further research to assess the effect of Acalypha indica Linn root extract on relative cell viability of hippocampal tissue posthypoxia with higher doses needs to be performed to obtain the optimal dose for the increase of post-hypoxic relative cell viability.…”

The combination of <em>Acalypha indica</em>–<em>Centella asiatica</em> extracts decreases the neuronal damage in hypoxia-induced hippocampal injury animal model

“…22 Medicinal plants have been known that contain several compounds besides polyphenol and flavonoids that support each other to provide a beneficial effect. 17 The root extract of AI has been used in several studies [6][7][8]11 and its compounds have been proven Vol. 27, No.…”

“…Previous studies have demonstrated that the root extract of Acalypha indica L. (AI) exhibited neuroprotective and neurotherapeutic effects on the neuromuscular junction and was able to inhibit the activity of neurotoxin on isolated frog tissues ex vivo 6 and in vivo. 7 The extract was also found to be effective in improving the rat hippocampal nerve cells at doses of 400 and 500 mg/kg BB. 8 On the other hand, Centella asiatica L. (CA) extract has been reported to be a peripheral vasodilator in neurological disorders and also improves circulation.…”

The Role of Acalypha Indica Linn. Extract on Heart Rates With Myasthenia Gravis Rat Model

“…In the previous study, the water extract of the roots of AI for animal models proved to have a neuroprotective effect [26]. For his studies, mechanisms of plant AI as therapies on MG were done by assessing the autonomic condition on heart muscle will be tested.…”