The relationship between sperm density, spermatocrit, sperm motility and fertilization success in Atlantic halibut, Hippoglossus hippoglossus

Tvedt, Harald B.; Benfey, Tillmann J.; Martin-Robichaud, D. J.; Power, J.

doi:10.1016/s0044-8486(00)00516-0

Cited by 91 publications

(52 citation statements)

References 27 publications

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“…The authors consequently relied solely on spectrophotometry to derive sperm density estimates for this species. However, a significant positive relationship was reported between halibut sperm density and spermatocrit (Tvedt et al, 2001). Differences among studies may be related to the ability to achieve homogeneity in diluted sperm concentrations.…”

Section: Sperm Densitymentioning

confidence: 94%

“…Dilutions of 1:250 or denser led to >80% fertilization success, yielding little among-male variability. Similar difficulty in achieving a broad range of fertilization rates was experienced in experiments with Atlantic herring (Clupea harengus) (Geffen, 1999) and Atlantic halibut (Hippoglossus hippoglossus) (9.4 × 10 5 4.6 × 10 8 spermatozoa/ egg, Tvedt et al, 2001). Using very high dilutions, for example, >1:1000 for cod, may generate very low fertilization rates or many zero values, also making among-male comparisons problematic.…”

Section: B) Experimental Design-semen Dilutionsmentioning

confidence: 95%

“…The two-step dilution procedure has also been used on winter flounder (Clearwater and Crim, 1998), though it was first proposed by Billard and Cosson (1992) for rainbow trout in order to trigger a synchronous movement of 100% spermatozoa and to easily observe a small quantity of cells. Sperm motility of Atlantic halibut was estimated by Tvedt et al (2001) by diluting 10 µl of milt in 1 ml of non-activating solution, and then 1 µl of this diluted solution was pipetted onto a microscope slide. Activation was achieved by adding 25 µl of seawater and a qualitative score to sperm motility assigned.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Ability to coordinate time of stripping with ovulation in flatfish is important so that eggs do not become over-ripened or released freely in the holding tank (Devauchelle et al, 1988). Use of GnRHa implants lowered sperm density in Atlantic halibut (Tvedt et al, 2001), whereas hormonal treatments used to induce ovulation in female summer and southern flounder were ineffective in inducing spermiation (Watanabe and Carroll, 2001). …”

Section: Broodstock Collection and Husbandrymentioning

confidence: 99%

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Estimation of Male Reproductive Success of Marine Fisheries

Trippel¹

2003

J. Northw. Atl. Fish. Sci.

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The subject of reproductive potential of fish populations is dominated by studies on the female gender. Studies on male reproduction are relatively few, but have increased in number during the previous decade. The objectives of this contribution were to describe and quantify the reproductive traits that make up the viable sperm production of a population. Some of these reproductive traits were easily measured from wild fish (e.g., mature testes weight), and others were more easily measured on captive fish (e.g., fertilization potential and sperm motility). Results of laboratory and field studies were then integrated to generate estimates of viable sperm production of a fish stock. A number of experimental protocols have been employed over the years to assess male fertility. The strengths and weaknesses of the different experimental approaches were reviewed and appropriate recommendations given towards establishing standardized protocols. Although the review is broad in nature, and includes references to a number of marine fishes, it concentrated on exploited species that occur in the North Atlantic and Baltic Sea within the taxonomic groups gadoidae, pleuronectoidae and clupeidae (Clupeiformes). In addition, published data on male reproductive traits of species in these taxa were tabulated and summarized. The terms of interest included sex ratio, maturity state, testes weight, sperm fertilization potential (artificial fertilization and paired mating), sperm density, sperm motility and paternal effects on early life history traits.An equation for determinate spawners was described that quantifies a population's viable sperm production using data on adult body characteristics and associated quantity and quality of sperm. The equation was used to establish time series of viable sperm production for Atlantic cod (Gadus morhua) of Newfoundland and Labrador. Results indicated on average 1 600 billion viable sperm were released to produce one recruit at age 3 years but with substantial variation among years in relation to changes in stock composition and growth as well as stock size. Distinct peaks in the number of viable sperm in 1981 and 1986 corresponded to distinct peaks in the number of age 3 recruits in those same years.

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Section: Sperm Densitymentioning

confidence: 94%

Section: B) Experimental Design-semen Dilutionsmentioning

confidence: 95%

Section: Sample Preparationmentioning

confidence: 99%

Section: Broodstock Collection and Husbandrymentioning

confidence: 99%

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Estimation of Male Reproductive Success of Marine Fisheries

Trippel¹

2003

J. Northw. Atl. Fish. Sci.

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“…A qualidade dos gametas e os métodos de fertilização artificial têm sido frequentemente avaliados, principalmente por meio de parâmetros que influenciam diretamente as taxas de fertilização, como duração da motilidade espermática (Tvedt et al, 2001), relação espermatozoide:ovócito (Bombardelli et al, 2006a) e diluição empregada no material fertilizante (Lahnsteiner et al, 2003). Esses parâmetros, quando manejados corretamente, melhoram significativamente as taxas de fertilização (Chereguini et al, 1999).…”

Section: Introductionunclassified

Dose inseminante para fertilização artificial de ovócitos de dourado

et al. 2009

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RESUMO -Objetivou-se determinar a dose inseminante adequada para uso na fertilização artificial de ovócitos de dourado (Salminus brasiliensis). Os ovócitos foram distribuídos em delineamento inteiramente casualizado, e fertilizados com uma das relações espermatozoides/ovócito 6,0×10 3 ; 6,0×10 4 ; 6,0×10 5 ; 6,0×10 6 ou 3,0×10 7 , cada uma com quatro repetições.Considerou-se unidade experimental uma incubadora de volume útil de 2,5 L, contendo 2,0 mL de ovócitos não-hidratados.As taxas de fertilização foram mensuradas 8 horas após o início da fertilização. Com intuito de verificar possíveis efeitos da diluição seminal na movimentação dos espermatozoides, realizou-se a mensuração do tempo de duração da motilidade espermática dos espermatozoides de dourado, ativados por meio de diferentes relações de diluição: 6,8×10 -5 ; 6,8×10 -4 ; 6,8×10 -3 ; 6,8×10 -2 ; 3,4×10 -1 e 1,0 mL de sêmen por mL de água. O tempo de duração da motilidade foi avaliado em delineamento inteiramente casualizado composto de seis tratamentos e três repetições. As taxas de fertilização apresentaram relação quadrática com o número de espermatozoides por ovócito. As relações de diluição do sêmen tiveram efeito inversamente proporcional sobre a duração da motilidade espermática. A relação que proporcionou melhores taxas de fertilização artificial Insemination dose for artificial fertilization of dourado oocytesABSTRACT -The objective of the present study was to determine the proper insemination dose of dourado (Salminus brasiliensis) oocytes. The oocytes were placed in a randomized complete design and fertilized with one of the spermatozoa.oocytes -1 ratio, 6.0×10 3 , 6.0×10 4 , 6.0×10 5 , 6.0×10 6 , 3.0×10 7 SPZ:OOC, each one with four replications. An experimental unit was considered to be an incubator with a 2.5L useful volume containing 2.0 mL non-hydrated oocytes. The fertilization rates were measured eight hours after the start of fertilization. In order to ascertain the possible effects of seminal dilution on the spermatozoa motility, the duration time of the spermatic motility of the dourado spermatozoa was measured when activated by different dilution ratios 6.8×10 -5 ; 6.8×10 -4 ; 6.8×10 -3 ; 6.8×10 -2 ; 3.4×10 -1 and 1.0 mL semen.mL water -1 . The motility duration time was assessed in a randomized complete design, with six treatments and three repetitions. The fertilization rates showed a quadratic relationship with the number of spermatozoids per oocyte. The semen dilution ratios had an inversely proportional affect on the spermatic motility time. The spermatozoa:oocytes ratio that provided the best artificial fertilization rates of dourado (Salminus brasiliensis) oocytes was 30,722 spermatoids per oocyte.

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