The Synthesis of Cytosolic Ascorbate Peroxidases in Germinating Seeds and Seedlings of Soybean and Their Behavior under Flooding Stress

Nishizawa, Keito; Hiraga, Susumu; Yasue, Hiroshi; Chiba, Mitsuru; Tougou, Makoto; Nanjo, Yohei; Komatsu, Setsuko

doi:10.1271/bbb.130384

Cited by 7 publications

(4 citation statements)

References 37 publications

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“…Peroxidases have been reported to be in lower abundance under flooding stress (Shi et al, 2008 ; Komatsu et al, 2010 , 2012 ). In soybean root, peroxidase genes have been reported to be downregulated under flooding stress (Nishizawa et al, 2013 ). Under flooding stress, the soybean seedlings show reduced growth (Hashiguchi et al, 2009 ).…”

Section: Resultsmentioning

confidence: 99%

Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress

Mustafa

Komatsu

2014

Front. Plant Sci.

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Flooding stress has a negative impact on soybean cultivation because it severely impairs growth and development. To understand the flooding responsive mechanism in early stage soybeans, a glycoproteomic technique was used. Two-day-old soybeans were treated with flooding for 2 days and roots were collected. Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress. Glycoproteins were enriched from total protein extracts using concanavalin A lectin resin and analyzed using a gel-free proteomic technique. One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively. Functional categorization of these identified glycoproteins indicated that the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress. Also the accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress. Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress. mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress. These results suggest that flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation; however glycoproteins involved in glycolysis are activated.

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Section: Resultsmentioning

confidence: 99%

Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress

Mustafa

Komatsu

2014

Front. Plant Sci.

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“…APX1 also gradually increased in the hypocotyls, roots and cotyledons during germination (Fig. 5) and seedling growth, and was shown to lead to increased degradation of seed storage proteins β-conglycinin and glycinin [63,64]. APX1 is synthesized during germination and seedling growth, similar to cytosolic APXs of Japanese radish [65] and rice [66].…”

Section: Validation Of Differentially Identified Proteinsmentioning

confidence: 96%

Gel-free quantitative proteomic approach to identify cotyledon proteins in soybean under flooding stress

Kamal

Hamid

Sakata

et al. 2015

Journal of Proteomics

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“…Moreover, cell death increased in the soybean root-tip under silver NPs mixed with nicotinic acid and KNO 3 (Figure 3). In soybean, ascorbate peroxidase was suppressed under flooding stress [48]; thereby, it induced the oxidative stress [49]. These results suggest that ubiquitin proteasome system is regulated under silver NPs mixed with nicotinic acid and KNO 3 by increasing the oxidative stress tolerance in order to maintain the cellular integrity; suggesting that growth might be improved under flooding stress.…”

Section: Role Of Proteasome Degradation Proteins Under Silver Nps Nimentioning

confidence: 87%

Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

Hashimoto

Mustafa

Nishiuchi

et al. 2020

IJMS

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Extensive utilization of silver nanoparticles (NPs) in agricultural products results in their interaction with other chemicals in the environment. To study the combined effects of silver NPs with nicotinic acid and potassium nitrate (KNO3), a gel-free/label-free proteomic technique was used. Root length/weight and hypocotyl length/weight of soybean were enhanced by silver NPs mixed with nicotinic acid and KNO3. Out of a total 6340 identified proteins, 351 proteins were significantly changed, out of which 247 and 104 proteins increased and decreased, respectively. Differentially changed proteins were predominantly associated with protein degradation and synthesis according to the functional categorization. Protein-degradation-related proteins mainly consisted of the proteasome degradation pathway. The cell death was significantly higher in the root tips of soybean under the combined treatment compared to flooding stress. Accumulation of calnexin/calreticulin and glycoproteins was significantly increased under flooding with silver NPs, nicotinic acid, and KNO3. Growth of soybean seedlings with silver NPs, nicotinic acid, and KNO3 was improved under flooding stress. These results suggest that the combined mixture of silver NPs, nicotinic acid, and KNO3 causes positive effects on soybean seedling by regulating the protein quality control for the mis-folded proteins in the endoplasmic reticulum. Therefore, it might improve the growth of soybean under flooding stress.

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The Synthesis of Cytosolic Ascorbate Peroxidases in Germinating Seeds and Seedlings of Soybean and Their Behavior under Flooding Stress

Cited by 7 publications

References 37 publications

Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress

Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress

Gel-free quantitative proteomic approach to identify cotyledon proteins in soybean under flooding stress

Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

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