2005
DOI: 10.1364/opex.13.004492
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Three-dimensional imaging and recognition of microorganism using single-exposure on-line (SEOL) digital holography

Abstract: We address three-dimensional (3D) visualization and recognition of microorganisms using single-exposure on-line (SEOL) digital holography. A coherent 3D microscope-based Mach-Zehnder interferometer records a single on-line Fresnel digital hologram of microorganisms. Three-dimensional microscopic images are reconstructed numerically at different depths by an inverse Fresnel transformation. For recognition, microbiological objects are segmented by processing the background diffraction field. Gabor-based wavelets… Show more

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Cited by 196 publications
(99 citation statements)
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“…In a serial work Javidi et al (2005Javidi et al ( , 2006aJavidi et al ( , c, d, e, f, 2010a, Moon and Javidi (2005, 2006, Yeom et al ( , 2007, , a 3D multi-class classifica-tion system is developed to classify different categories of WMs with a wide set of CBMIA techniques, including image segmentation, edge detection, shape features extraction, Gabor feature extraction, feature selection, inverse Fresnel transformation, maximum likelihood estimation, statistical inference, single exposure online digital holography, graph matching and partially temporal incoherent light in-line methods. In the experiments, numerical evaluations for the system is given, where Mean-squared Distance (MSD) and Mean-absolute Distance (MAD) are used to measure the classification effectiveness, where three classes of WMs are mainly tested (100 positive and 100 negative images of each class), finally a mean MSD around 1.5% and a mean MAD around 10% are achieved.…”
Section: Original Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…In a serial work Javidi et al (2005Javidi et al ( , 2006aJavidi et al ( , c, d, e, f, 2010a, Moon and Javidi (2005, 2006, Yeom et al ( , 2007, , a 3D multi-class classifica-tion system is developed to classify different categories of WMs with a wide set of CBMIA techniques, including image segmentation, edge detection, shape features extraction, Gabor feature extraction, feature selection, inverse Fresnel transformation, maximum likelihood estimation, statistical inference, single exposure online digital holography, graph matching and partially temporal incoherent light in-line methods. In the experiments, numerical evaluations for the system is given, where Mean-squared Distance (MSD) and Mean-absolute Distance (MAD) are used to measure the classification effectiveness, where three classes of WMs are mainly tested (100 positive and 100 negative images of each class), finally a mean MSD around 1.5% and a mean MAD around 10% are achieved.…”
Section: Original Methodsmentioning
confidence: 99%
“…In Ochoa et al (2007), a one-class classification approach is proposed to detect nematodes, where contour energy component values are encoded to represent shape information Javidi et al (2005Javidi et al ( , 2006aJavidi et al ( , c, d, e, f, 2010a, Moon and Javidi (2005, 2006, Moon et al (2009Moon et al ( , 2010, , , Yeom et al (2007) Classification results for nematode (green) and non-nematode contours (red) some nonnematode contours were removed to improve visibility (Ochoa et al 2007). (Color figure online) of nematodes, and a matching method is used to classify each of these individual SMs.…”
Section: Overview Of Sm Classificationmentioning
confidence: 99%
“…Yeom et al [102,31] use Gabor filtering on voxelized objects. The resulting features are matched using a dynamic link association which is composed of rigid motion of a graph followed by elastic deformation.…”
Section: Spectral Methodsmentioning
confidence: 99%
“…1. Using digital in-line holographic microscopy or digital holographic microscopy based on Mach-Zehnder interferometry, the shapes, sizes, and 3-D positions of living organelles were measured [14][15][16][17][18][19][20][21][22]. However, previous works have only focused on the 2-D imaging of phytoplankton and 3-D internal structures of individual live phytoplankton have not been measured yet, largely owing to the limitation of tomographic capability of conventional in-line holography or digital holographic microscopy.…”
Section: Introductionmentioning
confidence: 99%