Tumor Ablation with Irreversible Electroporation

Al-Sakere, Bassim; André, Franck; Bernat, Claire; Connault, Elisabeth; Opolon, Paule; Davalos, Rafael V.; Rubinsky, Boris; Mir, Lluis M.

doi:10.1371/journal.pone.0001135

Cited by 433 publications

(303 citation statements)

References 41 publications

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“…Our algorithm is a step towards effective treatment planning, not only in clinical ECT, but also in other electroporation based treatments, such as gene electrotransfer, transdermal drug delivery and irreversible tumor ablation. [16][17][18] …”

Section: Discussionmentioning

confidence: 99%

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Optimization of electrode position and electric pulse amplitude in electrochemotherapy

Županič¹,

Čorović²,

Miklavčič³

2008

Radiology and Oncology

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Section: Discussionmentioning

confidence: 99%

“…Our optimization algorithm successfully found the best parameters in all cases and with some further improvement it could be a useful tool in clinical ECT treatment planning as well as in treatment planning of other electroporation based treatments. [16][17][18] Županič A et al / Optimization of electroporation in electrochemotherapy …”

Section: Introductionmentioning

confidence: 99%

Optimization of electrode position and electric pulse amplitude in electrochemotherapy

Županič¹,

Čorović²,

Miklavčič³

2008

Radiology and Oncology

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“…[28][29][30][31] Electroporation has mainly been explored for cell transformation and studies of pore formation, [30][31][32] but lately it has been adopted in other elds, notably for tumor ablation. 33 The occurring membrane destabilization phenomenon can be reversible and transient or irreversible resulting in cell lysis. 34 This phenomenon has been described as Maxwell deformation, 31 where the lipid bilayers are reorganized as a response to an applied voltage.…”

Section: 23mentioning

confidence: 99%

Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip

Matos

Senkbeil

Mendonça

et al. 2013

Analyst

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Due to the extensive use of nucleic acid and protein analysis of bacterial samples, there is a need for simple and rapid extraction protocols for both plasmid DNA and RNA molecules as well as reporter proteins like the green fluorescent protein (GFP). In this report, an electropermeability technique has been developed which is based on exposing E. coli cells to low voltages to allow extraction of nucleic acids and proteins.The flow-through electropermeability chip used consists of a microfluidic channel with integrated gold electrodes that promote cell envelope channel formation at low applied voltages. This will allow small biomolecules with diameters less than 30 A to rapidly diffuse from the permeabilized cells to the surrounding solution. By controlling the applied voltage, partial and transient to complete cell opening can be obtained. By using DC voltages below 0.5 V, cell lysis can be avoided and the transiently formed pores can be closed again and the cells survive. This method has been used to extract RNA and GFP molecules under conditions of electropermeability. Plasmid DNA could be recovered when the applied voltage was increased to 2 V, thus causing complete cell lysis.

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“…41. Electrochemotherapy is obtained by a low number of successive short lived pulses (100 μs) while IRE requires a much longer treatment.…”

Section: Cellular Responsesmentioning

confidence: 99%

Time dependence of electric field effects on cell membranes. A review for a critical selection of pulse duration for therapeutical applications

Teissié¹,

Escoffre²,

Rols³

et al. 2008

Radiology and Oncology

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Background. Electropulsation is one of the non-viral methods successfully used to transfer drugs and genes into living cells in vitro as in vivo. This approach shows promise in field of gene and cellular therapies. This presentation first describes the temporal factors controlling electropermeabilization to small molecules (< 4kDa) and then the processes supporting DNA transfer in vitro. The description of in vitro events brings our attention on the processes occurring before (s), during (ms) and after electropulsation (ms to hours) ofhas the main advantages of being easy to use, fast, reproducible and safe.While during 30 years due to technological limits, pulse duration was always larger than 1 microsecond, the recent availability of high voltage (tens of kV) nanosecond long pulse generators opens the way to a new approach. Very fast perturbations under strong fields are induced in the membrane organization. 16,17 A new field of development is now present for electropermeabilization and promising results for clinical applications were reported.One of the limiting problems remains that very few is known on the physicochemical mechanisms supporting the reorganisation of the cell membrane. The molecular target of the field effect remains unclear.The present review focuses on the critical role played by the pulse duration in the electropermeabilization to small molecules (< 4kDa) and on its support to the processes associated to DNA transfer in vitro. Pulse durations are easy to adjust for an optimization of the clinical target: electrochemotherapy, irreversible electropermeabilization or gene therapy as suggested as a final conclusion. Electropermeabilization Theory of membrane potential difference modulation.An external electric field modulates the membrane potential difference. 18 From the physical point of view, a cell can be described as a spherical capacitor which is charged by the external electrical field. The transmembrane potential difference induced by the electric field, ΔΨi is a complex function g(λ) of the specific conductivities of the membrane (λ m ), the pulsing buffer (λ out ) and the cytoplasm (λ cyt ), the membrane thickness and the cell size. Thus, 1 ΔΨi = f. g (λ). r. E.cosθ [1] in which θ designates the angle between the direction of the normal to the membrane at the considered point on the cell surface and the field direction, E the field intensity, r the radius of the cell and f, which is a shape factor (a cell being a spheroid). Therefore, ΔΨi is not uniform on the cell surface. It is maximum at the positions of the cell facing the electrodes. These physical predictions were checked experimentally by videomicroscopy by using the potential difference sensitive fluorescent probes. [19][20][21] The pulse duration plays a critical role when shorter than the capacitive loading time of the membrane. In the previous part of the paper, it was considered that the pulse was long enough to bring the potential steady state value. The loading time τ load brings a limit in this description. 1 ΔΨi = f. ...

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Tumor Ablation with Irreversible Electroporation

Cited by 433 publications

References 41 publications

Optimization of electrode position and electric pulse amplitude in electrochemotherapy

Optimization of electrode position and electric pulse amplitude in electrochemotherapy

Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip

Time dependence of electric field effects on cell membranes. A review for a critical selection of pulse duration for therapeutical applications

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