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The current study amid to investigate association of Interleukin-17A with uropathogenic Escherichia coli among patients with urinary tract infection in karbala province, Iraq. Bacterial infections are widespread in urinary tract infections with a global extention. Uropathogenic E.coli (UPeC) is the most common causes of these infections. Out of 110 patients were examined by urologists for urinary tract infection, 25 patients showed positive result for UPEC and other 25 showed positive result for other bacterial pathogens. UPEC were diagnosed depended on the cultural, microscopical, biochemical examinations and confirm the identification by using Vitek2 system. Polymerase chain reaction was used to detection of four genes (pap C, cnfA, fim H, and fyu A). Interleukin-17A concentration in urine was measured by using ELISA kit. out of 110 urine samples, 56 (44.90%) with significant bacteriuria, 44(40%) with non-significant bacteriuria and 10 (9.09 %) with negative culture. The presence of UPEC among significant bacteriuria was 25/56 (44.64 %). The distribution of pap C, cnfA, fim H, and fyu A genes among UPEC were 17(68%), 17(68%), 16(64%) and 15(60%) respectively. Through UTI patients, 50 gave positive (121.70) pg/ml results compared to 30 of control (13. 94) pg/ml. Among uropathogenic Escherichia coli patients, 25 gave positive (92.80) pg/ml results, while 25 of other bacterial pathogens gave positive (15.40) pg/ml results.

Background: There are few studies had addressed the association between serum levels of Interleukin -22 and Interleukin-23 with severity of asthma, this study aims to evaluate whether elevated IL-22 and IL-23 serum levels is related to the severity of asthma. Methods: This study is a case-control study that included 60 asthmatic children (35 male and 25 female) as the patients group age ranged between 4 months to 14 years old, and 60 non-asthmatic (32 male and 28 female) age-matched patients group attending the outpatient clinic were recruited as control group. Serum levels of IL-22 and IL-23 was measured by sandwich ELISA using ELISA kits. Results: The results of this study have shown an elevated serum level of IL-23 in patients group compared with control group with significant (p= 0.008) and there was no significant difference between patients and control regarding IL-22 serum level (p = 0.721) in asthmatic children. Conclusion: According to the current study the serum level of IL-23 showing a significant association with the asthma severity in children.

The roles of interleukin 25 (a member of the interleukin 17 family) and interleukin 33 (a member of the interleukin 1 family) in asthma and airway hyperresponsiveness are yet to be fully understood. The aim of this study was to investigate the roles of IL- 25 and IL- 33 in the diagnosis of pediatric asthma and their association with the severity and treatment of the disease. This was a case-control study comprising 74 children with asthma as the patient group and 75 healthy children as the control group. The age of the participants ranged from 1 to 15 years. Levels of IL- 25 and IL- 33 in the serum were measured using ELISA kits. The highest positive predictive values (88.9%) occurred in IL- 25 with sensitivity and specificity of about 97.3% and 88.0% respectively, while the sensitivity and specificity of IL- 33 were about 51.4% and 66.0% respectively, with a positive predictive value of about (60.3%). The present study thus found that IL- 25 had higher diagnostic sensitivity and specificity values than IL- 33 in children with asthma. In addition, both interleukins were found to have a statistical significance regarding the treatment of the disease in children.

Background: celiac disease (CD) is an immune mediated disorder in genetic susceptible following ingestion of gluten containing product associated with increase serum level of many antibodies such as tissue transglutaminase.Materials and methods: Retrospective cross sectional study for five hundred patients with celiac disease their serum testes for ELISA tissue transglutaminase (tTg) IgA and IgG.Results: Higher serum level of tTg IgA was more than 200IU (37%) patients and 400 (80%) patients were less than 20IU while higher level of tTg IgG more than 5 IU in 222 (44%) patient. Conclusion:There was a significant relationship between tTg IgA serum level and age in celiac patients. Also, this study was reported a non-significant correlation between tTgIgG serum level and age of these patients. Key word: Celiac disease, tTg IgA, tTgIgG How to cite this article: Alattabi AS, Al-Hasnawi ATN, Ashour JA, et al (2020): Association of serum level tissue transglutaminase antibodies and age of Iraqi patients with celiac disease, Ann Trop Med & Public