Kefir is a dairy product commonly fermented by bacteria and yeasts. One of the potential vegetable milk materials to be used in kefir production is milk from red rice. Studies of fermentation of milk from red rice to produce kefir are barely found. This study aimed to determine the nutrient content of red rice milk kefir fermented using mixed of microorganisms, Lactobacillus bulgaricus and Candida kefir, in various concentrations of 3%, 9%, and 15%, respectively. The content of carbohydrate, fat, protein, water, and ash was measured using proximate analysis, while their content of lactic acid and alcohol was assayed using titration method. The results showed that fat, water and ash content in all resulted kefir products met standard quality of kefir, whereas carbohydrates and protein content did not. Treatment using the set mixed starters at a concentration of 9% was found to be able to produce kefir with a lactic acid content of 2.19%. Increasing alcohol content of kefir was dependent by starters. Generally, all treatments were able to produce kefir with alcohol content of 1.5% on average. As conclusion, fermentation of red rice milk at concentration of mixed starters at 9% has nutrient content which meet standard quality of kefir.

Pseudomonas spp. have been known as notorious food spoilage bacteria with ability to produce thermo-tolerant enzymes. They pose serious risk to public health as its most pathogenic member, P. aeruginosa, could cause nosocomial infections affecting peoplewith immunodeficiency. The use of GMF-GMR primers had been reported capable for detecting bacterial moaC of Alcaligenes javaensis JG3. The gene is suspected to be related with dormancy of pathogenic bacteria. This study aimed to investigate specificity of the GMR-GMF as well as a newly designed JMF-JMR pairs of primers (JMF: 5’- GGCGTACATCATCCACACTG-3’ and JMR: 5’-GGCGTTGACCATCTATGACA-3’) for detecting moaC genes of 57 members of Pseudomonas spp. retrieved from http://insilico.ehu.eus/ database using in silico PCR (Polymerase Chain Reaction). The results showed that GMF-GMR primers could selectively amplify 271-bp in silico PCR products from 14 out of 57 members of Pseudomonas spp. tested. However, BLASTn analysis on these 14 amplified DNA sequences showed that they were not part of moaC, yet glpK gene fragment sequences. Meanwhile, the newly designed primers from moaC sequence of strain JG3, JMFJMR, could specifically amplify 214-bp in silico PCR products from 2 out of 57 members of Pseudomonas spp. matched to bacterial moaC gene fragment sequences. As conclusion, based on in silico study JMF-JMR primers are more specific than GMF-GMR ones for detecting moaC gene fragments of members of Pseudomonas spp. studied.

Obesitas merupakan kelompok penyakit tidak menular yang menjadi kesehatan dunia dan muncul sebagai epidemi baik di negara maju ataupun berkembang. Konsumsi gula yang berlebihan diketahui merupakan faktor risiko obesitas dan dapat berujung pada penyakit Diabetes Mellitus Type 2 (T2DM)dengan angka mortalitas yang tinggi. Salah satu upaya untuk mengurangi kebiasaan mengkonsumsi gula berlebih dalam upaya mencegah obesitas sesuai anjuran pemerintah adalah penyuluhan tentang pentingnya pembatasan konsumsi gula bagi kesehatan. Upaya ini juga telah dilakukan melalui Program Pembangunan Kesehatan Masyarakat, PPKM) kepada masyarakat Desa Sumberlerak, Kabupaten Boyolali, Jawa Tengah, Indonesia, menggunakan poster sebagai media sosialisasi. Tujuannya adalah untuk meningkatkan masyarakat di desa tersebut terkait hubungan konsumsi gula berlebih dengan risiko dan gejala klinis dari obesitas dan DM. Pendekatan partisipatif berupa penyuluhan dengan media poster buatan mahasiswa telah dilakukan diikuti evaluasi berupa pengisian kuesioner sebelum dan sesudah penyuluhan. Berdasarkan hasil analisis kuesioner yang telah dilakukan, PPKM berupa penyuluhan menggunakan poster sebagai media komunikasi dapat meningkatkan kesadaran tentang bahaya konsumsi gula berlebih pada masyarakat Desa Sumberlerak, Kabupaten Boyolali Jawa Tengah.

Liquid biomedical waste is a form of medical waste from community health centers (Pusat Kesehatan Masyarakat or Puskesmas) with high levels of health hazardous organic contaminants. Bioremediation is an alternative way to eliminate toxic components in liquid waste. A bacteria community that can be used as component of organic waste bioremediation is indigenous hydrolytic and non-pathogenic to low-pathogenic bacteria. From previous studies, 4 hydrolytic indigenous bacterial isolates with such characteristics were obtained from liquid clinical wastes of two health centers in Semarang City, namely H1, H3, H5 (from Puskesmas Halmahera), and T3 (from Puskesmas Tlogosari Kulon). This study aimed to reveal the molecular identity and kinship these bacterial isolates to understand more of their properties as consortium of bioremediation agent. Molecular identification and phylogenetic tree construction works were carried out based on 16S rRNA gene sequences. Sequences of 16S rRNA gene sequences were obtained by isolation and gene amplification using the PCR method followed by sequencing. Based on the results of molecular identification, the four isolates studied were in the same class, namely Gammaproteobacter with Phylum Proteobacter. H1 bacterial isolates have 98.01% similarity with Acinetobacter schindleri. H3 and H5 isolates share the same genus, Stenotrophomonas, with 99.79% similarity with S. maltophiphila and 97.69% with S. acidaminiphila, respectively. T3 isolate had a similarity of 98.85% with Pararheinheimera aquatica species, which was widely known as a potent bioremediation agent. The phylogenetic tree design with the MEGA 6 program showed that the H3 and H5 isolates had the closest kinship compared to the other two isolates, while the T3 isolates had the farthest relationship with the 3 other isolates. This is in line with the fact that T3 isolate was originated from a different location separating it from 3 other isolates.