Since their discovery in 1985, fullerenes have attracted considerable attention. Their unique carbon cage structure provides numerous opportunities for functionalization, giving this nanomaterial great potential for applications in the field of medicine. Analysis of the chemical, physical, and biological properties of fullerenes and their derivatives showed promising results. In this study, functionalized fullerene based nanomaterials were characterized using near infrared spectroscopy, and a novel method - Aquaphotomics. These nanomaterials were then used for engineering a new skin cream formula for their application in cosmetics and medicine. In this paper, results of nanocream effects on the skin (using near infrared spectroscopy and aquaphotomics), and existing results of biocompatibility and cytotoxicity of fullerene base nanomaterials, are presented.

Background: Fullerene water complex establishes the optimal order and function of biomolecules in natural, biophysical way by transducing the signal through water hydrogen bonds to biomolecules.Objectives: This paper considers the effects of the patented hyper-harmonized-hydroxylated fullerene water complex (3HFWC) on biophysical properties of the skin collagen molecules.Method: Optomagnetic imaging spectroscopy (OMIS) has been used for the analysis of the biophysical skin properties (diamagnetic/paramagnetic) after applying three groups of different cosmetic products. Tested cosmetic products were prepared by replacing the active ingredients with 3HFWC or with water in four commercial products. The original commercial creams and their vehiculums with water added served as control groups. Data were statistically analyzed using paired t test in R software.Results: t Test gave statistically significant results for all of the products with 3HFWC, while within the control group, only body lotion and hand cream did show statistically significant results (P < 0.05). Significant improvements in abundance and quality of collagen in the dermis were achieved with body lotion with 3HFWC (p + /p − ranged from 0.82 to 0.97). While body lotion vehiculum made collagen-water interaction more stable (p + /p − ranged from 0.3 to 0.55), hand cream with 3HFWC made it more dynamic (p + /p − ranged from 0.63 to 0.49). Body lotion vehiculum improved the compactness of the dermis (p + /p − ranged from 0.2 to 1.03), as well as commercial hand cream (p + /p − ranged from 0.28 to 0.85). Conclusion:Compared to the control groups, cosmetic products with 3HFWC demonstrated positive effects on the biophysical properties of the skin. Increased paramagnetic properties are linked to more unpaired electrons, their faster movement, and, finally, better signal transduction. Thus, products with 3HFWC could enable faster regeneration of collagen and prompt skin reaction to the negative environmental influences.

Background Hyper Harmonized Hydroxyl Modified Fullerene Substance (3HFWC+) establishes hydrogen bonds with the surrounding water molecules and organizes them in clusters with the liquid crystalline state, similar to the properties of water surrounding the biomolecules. Aims To investigate the moisturizing properties of hyperharmonized fullerenol—3HFWC+ as an emulsion O/W ingredient on the skin. Patients/Methods We have analyzed the reflexion of the blue light from the skin, in vivo, with different levels of moisturization and compared the influence of three groups of cosmetic products (with various active ingredients, 3HFWC+ or water in same percentage in the “vehiculum”) on skin moisturization by measuring paramagnetic/diamagnetic properties by Optomagnetic Imaging Spectroscopy. Results Regenerating Cream and Body Lotion have shown statistically significant increase of diamagnetic features predominantly in all 3 types of creams, while the Hand Cream formulations have not shown statistically significant results after 4 weeks of treatment. Anti‐aging cream with 3HFWC+ demonstrated statistically significant increase of paramagnetic properties of the skin (p+/p‐ from 0.90 to 0.62) and showed beneficial effects on information transfer and water content in stratum corneum. Conclusion Although it does not act as a classical moisturizing agent on the skin, 3HFWC+ can increase moisturization as a result of hydrogen bonds established among 3HFWC+ and water or biomolecules, liquid crystalline state of water in 3HFWC+ resembling the properties of water surrounding the biomolecules, and finally, liquid crystalline state of water in the products.

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