One of the probiotic drinks produced by Scoby fermentation, namely Kombucha Honey Pineapple Subang, has been found as a source of gram-positive and gram-negative antibacterials in previous studies. In addition to its significance in the pharmaceutical (cosmetic) industry, Subang pineapple honey-based kombucha has the potential to be utilized as a functional food. Especially useful when making liquid body wash because it stops the growth of the skin-harming bacteria S. aureus, S. epidermidis, P. aeruginosa, and E. coli. The research was conducted with the intention of developing pharmaceutical biotechnology products in the form of liquid bath soap that contained the active ingredients of fermented kombucha solution and pineapple honey in concentrations of 15%, 25%, and 35%, respectively.This research method was a laboratory experiment using four soap-based preparations, including soap made from white sand with a sugar concentration of 15%, 25%, and 35%, a solution of fermented Kombucha Pineapple honey in Subang, and biore for market bath soap. The method used is disc diffusion. One-way ANOVA and post hoc analysis follow-up tests were used for data analysis. The study found that the most effective treatment for gram-positive and gram-negative bacteria was bath soap with a concentration of 35% of the active ingredient, kombucha fermented pineapple honey earring solution. The most recent advancement in preventing gram-positive and gram-negative bacteria from growing is the subject of this study.
Kombucha bunga telang selain berpotensi sebagai minuman probiotik dalam meningkatkan sistem kekebalan tubuh memiliki potensi juga sebagai bahan aktif sabun cuci tangan dalam menghambat pertumbuhan bakteri Staphylococcus epidermidis, Vibrio parahaemolyticus, dan Escherichia coli. Tujuan penelitian ini adalah untuk membuat formulasi dan sediaan sabun cuci tangan yang berbahan aktif larutan fermentasi kombucha bunga telang dengan konsentrasi gula 20%, 30%, dan 40%. Metode penelitian ini dilakukan secara eksperimental laboratorium dengan membuat 4 sediaan sabun cuci tangan dan menyediakan sabun cuci tangan pasaran untuk diuji pada ketiga pertumbuhan bakteri uji melalui metode difusi cakram. Analisis data yang digunakan adalah ANOVA satu jalur dilanjut melalui Analisis pos hoc. Hasil penelitian ini disimpulkan bahwa sabun cuci tangan yang berbahan aktif larutan fermentasi kombucha bunga telang berkolerasi secara positif dalam menghambat ketiga pertumbuhan bakteri uji. Konsentrasi 40% pada sediaan sabun cuci tangan kombucha bunga telang berbeda nyata secara signifikan dengan nilai F tabel lebih kecil dibanding F hitung (P<0,05) pada konsentrasi 20%, 30%, dan basis sabun. Namun tidak berbeda nyata dengan sabun cuci tangan yang tersedia dipasaran dalam menghambat ketiga pertumbuhan bakteri uji. Konsentrasi 40% (sabun cuci tangan kombucha bunga telang) konsentrasi terbaik dari seluruh perlakuan dalam menghambat ketiga pertumbuhan bakteri uji.
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