from Siwa), were subjected to DNA extraction and subsequently to SSR-PCR amplification. Heterozygosity and Wrights F-statistics (FIS, FST and FIT) were calculated to assess the genetic variation in these populations.In the present study, high values of FIS were detected in Siwa (83%) and Farafra (69%) cattle with moderate genetic differentiation (13%) between the two populations. A total number of 22 and 29 alleles with means 2.75±0.71 and 3.63±0.74 were observed in Siwa and Farafra cattle, respectively. Alleles observed per locus ranged between 2 (loci ILSTS054, ILSTS005 and ILSTS011) to 4 (locus HEL5) in Siwa cattle and between 3 (loci ILSTS005, HEL5, ILSTS006 and ILSTS011) to 5 (locus BM1818) in Farafra cattle populations. Mean values of observed and expected heterozygosities were 0.09± 0.27 and0.46 ± 0.21 for Siwa cattle, meanwhile it ranged from 0.20± 0.35to 0.66 ± 0.07 in Farafra cattle. Polymorphic information content value (PIC) ranged from 0.10 to 0.71 for marker ILSTS005 and HEL5, respectively, with a mean value of 0.45 for all loci in Siwa cattle. Moreover, its range was 0.52 (ILSTS054) to 0.74 (BM1818) for all loci with a mean of 0.64 in Farfra cattle. Population fixation indices traced about 0.653 variation referring to differences among individuals versus total variance (FIT), where it was the lowest among populations differences versus total variance (FST= 0.237) indicating low level of population differentiation. A pair-wise difference amongst Siwa and Farafra cattle populations was recorded (0.546) among populations (F index (FIS).Moreover, 4 and 11private alleles were observed in Siwa and Farafra cattle populations, respectively. Following that we are suggested the use of these alleles as population fingerprint and they could be used to differentiate these two populations.
Morphometric characterization is the basis of conservation strategies, performance improvement, breeding, and sustainable utilization plan. The purpose of this research was to determine the morphometrics of the Kacang Goats at the fishpond area in Sidoarjo Regency based on different ages and sex. The fishpond area in Sidoarjo is a closed area where goats are raised and have the potential for inbreeding with the consequence of smaller-sized offspring than normal. Morphometric measurements included the head, ears, neck, body, legs, tail, and horns. This research used a descriptive analysis approach, presenting data in form of mean, standard deviation, and coefficient of variation. The result showed that the average face length of male vs. female Kacang goats was 16.73 ± 2.40 cm vs. 19.68 ± 2.44 cm; with a face width of 11.25 ± 2.91 cm vs. 19.52 ± 3.18 cm; ear length of 14.62 ± 1.38 cm vs. 16.46 ± 1.47 cm; neck length of 15.53 ± 2.65 cm vs. 15.35 ± 2.14 cm; horn length of 7.41 ± 4.10 cm vs. 6.59 ± 2.89 cm; chest circumference of 59.33 ± 8.18 cm vs. 67.80 ± 6.32 cm; body length of 51.45 ± 6.70 cm vs. 56.91 ± 6.54 cm; body height of 54.41 ± 7.22 cm vs. 62.01 ± 5.53 cm; tail length of 13.21 ± 2.01 cm vs. 14.77 ± 3.37 cm; hind leg length of 39.20 ± 5.26 cm vs. 47.74 ± 5.47 cm; and front leg length of 37.00 ± 8.84 cm vs. 44.63 ± 8.65 cm. This research concludes that Kacang Goats in Sidoarjo has characteristics according to SNI standard, and the morphometric size was larger in female than male goats.
The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan's multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage.
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