Batik is an industry that is quite popular in Indonesia. Batik has been declared as one of Indonesia’s cultural heritage and is recognized by UNESCO. Batik production is recorded to always increase every year. Unfortunately, the batik industry in Indonesia is dominated by many small industries with limited fund management. This makes the environmental aspects and waste disposal neglected. Through this research, study and isolation were carried out to explore the potential of indigenous bacterial isolates that can biodegrade dyes in batik along with other physical parameters of waste. A sample that contained wastewater and sediment from batik industry are collected and cultured in 1000 ml Busnall Hass medium which olive oil-enriched and put at the shaker condition at 150 rpm until 6 days at 30°C. Identification of the isolates examined for their morphological, physiological and biochemistry test. Bacillus subtilis is one of the indigenous isolates from this research. This study aimed to determine the ability of B. subtilis to reduce BOD, COD, TSS, and pH in batik waste and present the results of the DNA analysis of B. subtilis. Based on the research that has been done, it can be concluded that the molecular identification of bacteria through analysis of the 16S rDNA gene fragment sequence, the bacteria that play a role in the degradation of dyes is Bacillus subtilis strain NAP1 with an average similarity level of 93%. B. subtilis NAP1. B. subtilis NAP1 isolate offers the potential for future bioremediation of batik wastewater.

This study aimed to determine the effect of using natural masks to moisturize dry skin. This natural mask was made of ingredients that are safe to use and based on special formulations which include; bengkoang powder (2 grams), chocolate powder (1 gram), black cumin oil (0.6 ml), and honey (5 ml). This formulation was made so that the resultied mask formed paste stucture that can be directly applied. Masks were given to ten probandal women who have dry skin types and are 30-40 years old. Masks were used twice / week for six weeks (12 times usage). The instrument used to measure the moisture level of facial skin was a skin analyzer (HL-611) by directly attaching it to the face area and the results can be seen after 4-5 seconds. The data were analysed using ANOVA test 95% confidence interval and significance test using SPSS 15 for windows. The results showed that skin moisture values increased around 3.9% within 6 weeks of use, with a 0.8% increase at week 2, at week 4 there was an increase of 1.65% and at week 6 there was an increase in skin moisture by 1.4%. The moisture value of the skin on the face area (forehead, nose, chin, cheeks) showed no significant difference with almost the same value of humidity. It is recommended for dry skin types to use a face mask that contains oil as a skin lubricant and to protect water evaporation from the skin. It is necessary to do further testing on this natural mask so that safety on the skin can be guaranteed. It needs to be done so that the final product obtained meets the face mask safety quality standards set by the government. Keywords: Bengkuang; cocoa; black cumin; honey; natural mask.

Abstract-Biodegradation Keywords:Biodegradation, In vitro, Tanning pollutants, Indigen bacteriaAbstrak-Biodegradasi dapat menjadi salah satu teknologi pengolahan air limbah yang aman dan berwawasan lingkungan. Secara alamiah, bakteri yang berpotensi dalam proses biodegradasi dapat diisolasi dari limbah itu sendiri. Bakteri inilah yang disebut sebagai bakteri indigen. Sungai Badheg di kota Malang merupakan salah satu sungai yang tercemar limbah pabrik kulit. Hasil uji kualitas air dari sampel menunjukkan kondisi yang tidak memenuhi baku mutu air, yaitu TSS sebesar 400 ppm, BOD sebesar 331 mg/L, COD sebesar 544 mg/L, DO sebesar 3,6 mg/L dan kadar lemak sebesar 280 mg/L. Limbah pabrik kulit memiliki karakteristik yang berbau tajam, berwarna hitam pekat, dan mengandung lemak dalam jumlah yang besar. Lemak diketahui sebagai salah satu bahan organik yang sulit untuk diuraikan secara alamiah. Peran bakteri indigen akan mengoptimalkan proses biodegradasi lemak yang terjadi. Kegiatan biodegradasi dilakukan dengan cara mengintroduksi bakteri indigen yang telah diseleksi dari proses isolasi dan memiliki potensi pendegradasi paling tinggi, kemudian dikultur dan diinokulasikan kembali pada sampel air, sehingga proses transformasi akan berlangsung lebih optimal. Hasil biodegradasi secara in vitro menggunakan bakteri indigen, menunjukkan perubahan TSS sebesar 293ppm, BOD 117 mg/L, COD sebesar 165 mg/L, DO sebesar 19 mg/L dan kadar lemak sebesar 0,02 mg/L. Biodegradasi merupakan cara yang efektif dalam mendegradasi bahan organik, terutama kadar lemak. PENDAHULUANEra perkembangan Biologi saat ini telah memasuki tahap baru, yang kecenderungannya secara global mengalami transisi dari pemanfaatan teknologi secara fisik dan kimia, berganti menjadi pemanfaatan yang mengedepankan era Biologi. Konsep yang ingin dicapai manusia sejalan dengan hal tersebut adalah kemajuan teknologi dan perkembangan informasi yang seirama dengan pemahaman terhadap lingkungan serta pengelolaan lingkungan, dan menunjukkan kecenderungan perubahan

The purpose of this study was to know the impact of the Genetics E-module to improve students’ motivation and learning outcomes during the learning process. The method applied was Research and Development, limited until the implementation stage, using the quasi-experiment method with one group pretest-posttest. The motivation instruments were modified questionnaire by Keller with ARCS model, consist of 4 aspects including attention, relevance, confidence, and satisfaction. The population were all of the students from class of 2017 in Biology Education department, Tribhuwana Tunggadewi State University. The pretest was held to know students’ motivation and learning outcomes when using a handout from the lecturer, while the posttest was to know students’ motivation and learning outcomes when using Genetics E-module. Data were collected from the motivation questionnaire and test. After using E-module, there were 27 students with high (71,91 ≤ x < 86), 18 students with medium (57,82 ≤ x < 71,91) and 5 students with low criterion (43,74 ≥ x) of motivation, and there were  58% students who passing the grade in Genetics test. Based on the data analysis, E-module was feasible to be used to improve students’ motivation and also learning outcomes in the Biology Education department.

ABSTRAK Mahasiswa dalam mempelajari sains, perlu memperhatikan hakikat sains. Hakikat Sains dipelajari mahasiswa dengan tujuan untuk mengembangkan kemampuan berpikir (mind on), keterampilan (hands on) serta sikap ilmiah (heart on). Kajian Mikrobiologi di prodi TIP, Universitas Tibhuwana Kata kunci: Biodegradasi in vitro, Bahan ajar, Bakteri Indigen ABSTRACT Students in the study of science, need to pay attention to the nature of science. Science studied to develop the ability to think (minds on), skills (hands on) as well as the scientific attitude (heart on). Study Microbiology in Prodi TIP, University Tibhuwana Tunggadewi is about microorganisms. The writers conducted research related to environmental remediation efforts to harness the potential of bacteria indigen as an agent in the process