No abstract

Background:- Colorectal carcinoma is the most common cancer after the breast cancer in female and bronchus cancer in male. P53 is a tumor suppressor gene, approximately half of colorectal cancers present mutation in p53 gene.Objectives:- To determine the frequency and the pattern of p53expression in colorectal carcinoma by immunohistochemical technique and to correlate this expression with different clinicopathological parameters.Materials and methods:-Thirty cases of colorectal carcinoma were included in this study, these cases were diagnosed in private pathology laboratories in Baghdad / Iraq from January 2015 to Jaune 2015. Clinicopathological parameters such as age , gender , pathological diagnosis , including the tumor site , lymph nodes status , grade and stage of tumor were taken from patients files.Sections of 4μm stained by hematoxylen and eosin stain and immunohistochemical stained for p53.Results: Nineteen (63.3%) of the cases were males, 11(36.7%) cases were females, with age distribution ranging from (39-89) years with a mean age of 56.5 years . Ten cases(33.3%) located in the cecum , 3(10%)cases from each right colon , sigmoid 8 (26.7%) cases were from left colon and 6(20%) cases were from the rectum. Histologically the tumor grade range from moderately differentiated in 27 (90%) cases, and poorly differentiated in 3 (10%) cases. Regarding pathological staging (TNM system),2 (6.7%) cases were T2 ,24 (80%) were T3 , 4(13.3%) cases were T4. Lymph node involvement found in 14(46.7%) cases, and distant metastasis was found in 3(10%) cases. P53 expression was present in 11(36.7 %) cases, were distributed as follows:- weak in 1(3.3%) case,moderat in 3(10%) and marked in 7(23.4%) cases. There was no correlation between p53 expression and the clinicopathological parameters age , sex , histopathological grade, location , lymph nodes status and tumor stage.Conclusion: There was no significant statistical correlation between P53 expression by tumor and different clinicopathological parameters in this study

Background: Colorectal carcinoma is the most frequent type of malignancy in terms of incidence,it is the third most common cancer world wide in both sexes . bcl_2 expression has been described as been a better prognostic factor in colorectal carcinoma.Objcetives: To determine the frequency and the pattern of Bcl-2 expression in colorectal carcinoma by immunohistochemical technique and to correlate this expression with different clinicopathological parameters.Material and method: Thirty cases of colorectal carcinoma were studied, these cases were diagnosed in private laboratories in Baghdad / Iraq from January 2015 to Juan 2015. Clinicopathological parameter such as age, gender , pathological diagnosis , tumor site , lymph nodes status , grade and stage of tumor were collected from patients files..Sections of 4μm were stained by hematoxylen and eosin stain, and immunohistochemical stained for Bcl-2.Results: Nineteen (63.3%) cases were males, 11(36.7%) cases were females, with age distribution ranging from (39-89) years with a mean age of 56.5 years. Histologically the tumor grade range from moderately differentiated in 27 (90%) cases and poorly differentiated in 3 (10%) cases. Ten (33.3%)cases located in the right colon and sigmoid each 6(20%) cases in rectum,8(26.7%) cases in the cecum,3(10%)cases located in right colon and sigmoid each,6(20%) cases in the rectum and 8(26.7%) cases in the left colon. Regarding pathological staging (TNM system),2 (6.7%) cases were stage T2 ,24 (80%) were stage T3 ,4(13.3%) cases were T4. Lymph node involvement found in 14(46%) cases and distant metastasis was found in 9(30%)cases. Bcl-2 expression was present in 14(46.7 %) cases of 30 colorectal carcinoma.There was no significant correlation with age, sex, histopathological grade, location, lymph nodes status and tumor invation.Conclusion: There was no significant statistical correlation between bcl expression by tumor cell of colorectal carcinoma and different clinicopathological parameters in this study.

HCMV has been detected in various types of tumors. Besides its well-describedoncomodulation, recently an oncogenic properties of HMCV per se as well asits direct transforming role in infected cells have been described.HCMV in serous ovarian adenocarcinoma and its relation to BRCA1and BRCA2 was scarcely studied.The study was designed to examine cellular dysregulation mediated by the concordant protein expressions of BRCA1 and BRCA2 tumor suppressor genes with HCMV in tissues from ovarian cancers.Eighty ovarian tissues were examined for HCMV-DNA and BRCA1 & BRCA2 genes expression. Those samples belonged to (40) patients diagnosed with ovarian cancer and (40) from apparently normal ovarian tissues. The detection of HCMV-DNA was done by chromogenic in situ hybridization (CISH) whereas the translated proteins of the expressed BRCA1 & BRCA2 genes by immunohistochemistry (IHC).Positive signals of HCMV-DNA -CISH reactions in malignant serous epithelial ovarian tumors, was detected in 45% (18 out of 40)tissues and followed by the apparently healthy ovarian control tissues (12.5%,5 out of 40 tissues). The difference of the HCMV in ovarian cancers and control was highly significant. The translated proteins of the expressed BRCA1 & BRCA2 genes was detected by IHC in 60% (24 out of 40 tissues) of malignant serous epithelial ovarian tumors while no signal was reported in the control tissues. The difference between the percentages of BRCA1 as well as BRCA2proteins detection in ovarian cancer & control group was statistically significant (<0.05).The significant detection of either HCMV o rBRCA1 & BRCA2 genes expression in the studied ovarian cancer tissues could support a role for that virus along with these genes in ovarian carcinogenesis.

Sinonasal malignant neoplasms are rare and heterogeneous tumors of sinonasal tract, constituting about 3% of the upper respiratory tract tumors. A variety of gene products encoded by Human herpes Virus -8,are involved in transformation, as well as promotion of oncogenesis. angiogenesis, anti-apoptosis, and immune modulation and evasion. The aim is to unravel the ratesof Human herpes Virus -8 (HHV-8) infections in tissues from a group of patients with sinonasal carcinoma (SNC) and sinonasal papilloma (SNP). Eighty-three formalin-fixed, paraffin-embedded sinonasal carcinoma (SNC) and sinonasal papilloma (SNP)tissues enrolled in this study; 18 sinonasal tissue biopsies from sinonasal carcinoma; 35 tissue biopsies from sinonasal papilloma and 30 apparently healthy nasopharyngeal tissues with unremarkable pathological changes, as control group. Detection of HHV-8 was done by new detection version of chromogenic in situ hybridization (CISH) technique. The positive CISH reactions for HHV-8-DNA were detected in 22.2% and 5.7% of sinonasal carcinoma and sinonasal papilloma tissues, respectively. And no control tissues presented positive signals for HHV-8-CISH test. The correlation between HHV-8 and sinonasal carcinoma and sinonasal papilloma was highly significant (P= 0.001). The significant HHV-8 detection rates in sinonasal carcinoma and sinonasal papilloma tissues might shade light for a possible role of this virus in either sinonasal pathogenesis or carcinogenesis.