Kulit yang mengalami penuaan dini menyebabkan nilai kelembaban kulit menjadi berkurang sehingga kulit menjadi kering. Berkurangnya nilai kelembaban kulit dapat diatasi dengan memberikan perawatan terhadap kulit. Perawatan dapat dilakukan dengan sediaan topikal yang mengandung antioksidan salah satunya yaitu masker gel peel off ekstrak kulit buah manggis (Garcinia mangostana L.). Penelitian pendahuluan ini bertujuan untuk melihat nilai kelembaban kulit manusia pada pemakaian masker gel peel off ekstrak kulit buah manggis. Pengujian dilakukan dengan menggunakan 5 subjek uji masing masing dioleskan plasebo pada tangan kanan dan dioleskan masker gel peel off ekstrak kulit manggis pada tangan kiri selama 14 hari. Metode perlakuan subjek uji dilakukan secara randomized, double blind, plasebo control group design. Data yang diperoleh diuji normalitas dan homogenitasnya. Data nilai kelembaban terhadap nilai sebelum dan sesudah penelitian dibandingkan dengan uji T Berpasangan. Hasil penelitian menunjukkan bahwa pada kelompok kontrol dan perlakuan menghasilkan nilai p<0,05 yang bermakna yang menunjukkan bahwa terdapat perbedaan yang bermakna sebelum dan sesudah dioleskan basis masker maupun masker yang mengandung kulit manggis. Kedua kelompok menunjukkan adanya perubahan, namun rentang peningkatan pada kelompok perlakuan lebih besar dibandingkan dengan kelompok kontrol. Dengan demikian, diketahui masker gel peel off Garcinia mangostana L. mampu meningkatkan nilai kelembaban kulit manusia lebih besar dibandingkan kelompok kontrol.

Background: Photoaging is a premature aging that occurs on skin due to the ultraviolet light exposure that causes the emergence of clinical symptoms, one of which is wrinkle. One kind of material that can prevent photoaging is antioxidant. A single clove garlic has the highest antioxidant activity compared to the other materials. Aim: This research aimed at investigating the effectivity of single clove garlic in inhibiting the clinical symptom of photoaging. Method: This research was an experimental research, utilizing pre-post-test control group design. The used sample were 30 male wistar rats which were divided into 6 experimental groups. All groups were exposed to UV-B light with the amount of 840 mJ/cm2. Control group (P1) was only exposed to UV-B while the other groups were treated with placebo cream (P2), sunblock (P3), 5% garlic cream (P4), 10% garlic cream (P5) and 20% garlic cream (P6) respectively. The clinical symptom in the form of wrinkle was observed by using dermascope and the observations were categorized based on Glogau Scale. The statistical analysis utilized Wilcoxon and Kruskal Wallis test which was followed by Mann Whitney test. Result: The result of the research showed that there was significant difference on the apparent wrinkle on P1 group and P3, P5 and P6. Meanwhile the comparison between P1, P2 and P4 was not significant. Conclusion: The single clove garlic extract can prevent photoaging and has the similar protective effect for the skin as of sunblock.

Background: The role of both Euphorbia milii and Propolis is to increase the effectiveness in pathological condition of the body part damaged, include renal function in maintaining the homeostasis. That combination into mice which are infected by Mycobacterium tuberculosis will change the total number of glomerulus in the kidney. Aim: The study aims to determine the effect of combination tea from Euphorbia milii and Propolis on the number of the glomerulus in mice infected by M. tb. Method: This study confirmed randomized post-test only control group design consisting of 24 mice divided into six groups (G1-G6). E. milli flowers are obtained from flower plantations, in Ketewel, Gianyar, Bali Province took in fresh and dark red sampling. M. tuberculosis used H37Rv strain (ATCC27294) grown on Middlebrook 7H9 liquid medium for two weeks. Result: It was found that Group 3 and Group 5 from the treatment group has the most significant average number of glomerulus which was 13, followed by Group 2 and Group 6 from the control group has the largest average number of glomerulus which was 12. The differences were not significant between groups with P = 0.463 (P <0.05). Conclusion: The Euphorbia milii tea and its combination with Propolis does not affect histological change on the total number of glomerulus in mice infected with M. tb.

Background: Aging and estrogen deficiency in menopause women increase susceptibility to liver diseases. In postmenopausal women, the change of ERα expressions and oxidative stress is related to liver diseases' pathogenesis. Phytoestrogens have been shown to protect and repair the liver organ due to estrogen deficiency. Purple Sweet Potatoes contain anthocyanins, a subgroup of flavonoids that have been shown to have antioxidant and phytoestrogen activity. This study aims to evaluate the phytoestrogen and antioxidant activities of purple sweet potato in a liver animal model of menopause through Superoxide Dismutase (SOD) and ERα expressions.Method: A true experimental study by randomized post-test-only control group design was conducted among 28 female Wistar rats-aged 10-12 weeks with a bodyweight of 180-220 gram, which underwent bilateral ovariectomies. These animals were divided into 4 groups, with 7 rats in each group. On day 7, Group T0, as control was given aquadest, ethanol extract of purple sweet potato was assigned to Group T1, T2, and T3 with doses 1, 2, and 4 mL/day, respectively, during 30 days. The SOD and ERα mRNA expressions were evaluated by qRT-PCR with specific primers. Data were analyzed using SPSS version 20 for Windows.Results: The mRNA expressions of ERα were not significantly different among groups (p>0.05), while SOD expressions were significantly different among groups (p<0.05). The expression of SOD in Group T1 (504.39±231.4 pg/μL), T2 (2,147.92±417.1 pg/μL), and T3 (405.30±224.1 pg/μL) showed a significantly higher than Group T0 (15.34±15.27) (p<0.05)Conclusion: Oxidative stress could be prevented by purple sweet potato in the liver through the SOD mRNA expression but not ERα.

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